贵州农业科学
貴州農業科學
귀주농업과학
GUIZHOU AGRICULTURAL SCIENCES
2010年
1期
1-4
,共4页
糯玉米%SSR标记%遗传多样性%聚类分析
糯玉米%SSR標記%遺傳多樣性%聚類分析
나옥미%SSR표기%유전다양성%취류분석
glutinous maize%SSR marker%genetic diversity%clustering analysis
为分析48份糯玉米自交系的遗传多样性及其之间的遗传关系,利用总DNA提取方法和微卫星分子标记(SSR)技术,取均匀分布各染色体的45对SSR引物对样品进行PCR扩增分析,筛选出23对扩增带型稳定的SSR引物,从供试材料中检测出141个等位基因变异,每对引物检测等位基因2～14个,平均5.48个.SSR引物的PIC介于0.43～0.89,平均多态性信息量为0.66.UPGMA方法聚类分析表明,供试材料间遗传距离变幅为0.26～0.72,平均值为0.49.SSR标记能将全部自交系区分开来,48份糯玉米自交系可分为六类.
為分析48份糯玉米自交繫的遺傳多樣性及其之間的遺傳關繫,利用總DNA提取方法和微衛星分子標記(SSR)技術,取均勻分佈各染色體的45對SSR引物對樣品進行PCR擴增分析,篩選齣23對擴增帶型穩定的SSR引物,從供試材料中檢測齣141箇等位基因變異,每對引物檢測等位基因2～14箇,平均5.48箇.SSR引物的PIC介于0.43～0.89,平均多態性信息量為0.66.UPGMA方法聚類分析錶明,供試材料間遺傳距離變幅為0.26～0.72,平均值為0.49.SSR標記能將全部自交繫區分開來,48份糯玉米自交繫可分為六類.
위분석48빈나옥미자교계적유전다양성급기지간적유전관계,이용총DNA제취방법화미위성분자표기(SSR)기술,취균균분포각염색체적45대SSR인물대양품진행PCR확증분석,사선출23대확증대형은정적SSR인물,종공시재료중검측출141개등위기인변이,매대인물검측등위기인2～14개,평균5.48개.SSR인물적PIC개우0.43～0.89,평균다태성신식량위0.66.UPGMA방법취류분석표명,공시재료간유전거리변폭위0.26～0.72,평균치위0.49.SSR표기능장전부자교계구분개래,48빈나옥미자교계가분위륙류.
The genetic diversity of 48 glutinous maize inbred lines and the genetic relationship between these lines were analyzed by the SSR molecular marker and total DNA extraction method. 23 pairs of stable SSR primers with the amplification band pattern were amplified from 45 pairs of SSR primers of samples. 141 alleles were detected from 48 tested inbred lines and 2～4 alleles with average 5.48 alleles were detected from a pair of primer. The polymorphism information content and mean polymorphism information content of each SSR primer was 0.43～0.89 and 0.66. The results from UPGMA clustering analysis showed that the genetic distance between tested lines was 0.26～0.72 and its mean value was 0.49.48 The glutinous maize inbred lines can be divided into six groups, which can provide a reference for its breeding.
