西北农业学报
西北農業學報
서북농업학보
ACTA AGRICULTURAE BOREALI-OCCIDENTALIS SINICA
2010年
2期
25-28
,共4页
邱瑾丽%李引乾%周广辉%胡延春%刘磊%熊永洁%刘金鑫%王盟
邱瑾麗%李引乾%週廣輝%鬍延春%劉磊%熊永潔%劉金鑫%王盟
구근려%리인건%주엄휘%호연춘%류뢰%웅영길%류금흠%왕맹
多种微量元素注射液%小鼠%免疫功能
多種微量元素註射液%小鼠%免疫功能
다충미량원소주사액%소서%면역공능
Trace elements injection%Mice%Immunologic funtion
将60只昆明小鼠(体质量为18~22 g,雌雄各半)随机分为3组,每组20只.再将每组的20只分成试验组和对照组,每组10只,试验组每天腹腔注射0.04 mL"多种微量元素注射液",对照组注射0.04 mL生理盐水,连续注射7 d .3组分别进行酸性α-醋酸萘脂酶法试验,溶血空斑试验和血清溶血素抗体(HC_(50))测定.结果表明,试验组小鼠ANAE阳性细胞百分比为51.80%±3.645%,极显著高于对照组的39.75%±4.283%(P<0.01);试验组小鼠溶血空斑形成数量为23.1±2.13177,极显著高于对照组的17.4±2.01108(P<0.01);试验组小鼠血清溶血素水平为262.2900±10.48257,显著高于对照组的231.2710±39.61145(P<0.05).即"畜禽用多种微量元素注射液"能显著提高小鼠的免疫功能.
將60隻昆明小鼠(體質量為18~22 g,雌雄各半)隨機分為3組,每組20隻.再將每組的20隻分成試驗組和對照組,每組10隻,試驗組每天腹腔註射0.04 mL"多種微量元素註射液",對照組註射0.04 mL生理鹽水,連續註射7 d .3組分彆進行痠性α-醋痠萘脂酶法試驗,溶血空斑試驗和血清溶血素抗體(HC_(50))測定.結果錶明,試驗組小鼠ANAE暘性細胞百分比為51.80%±3.645%,極顯著高于對照組的39.75%±4.283%(P<0.01);試驗組小鼠溶血空斑形成數量為23.1±2.13177,極顯著高于對照組的17.4±2.01108(P<0.01);試驗組小鼠血清溶血素水平為262.2900±10.48257,顯著高于對照組的231.2710±39.61145(P<0.05).即"畜禽用多種微量元素註射液"能顯著提高小鼠的免疫功能.
장60지곤명소서(체질량위18~22 g,자웅각반)수궤분위3조,매조20지.재장매조적20지분성시험조화대조조,매조10지,시험조매천복강주사0.04 mL"다충미량원소주사액",대조조주사0.04 mL생리염수,련속주사7 d .3조분별진행산성α-작산내지매법시험,용혈공반시험화혈청용혈소항체(HC_(50))측정.결과표명,시험조소서ANAE양성세포백분비위51.80%±3.645%,겁현저고우대조조적39.75%±4.283%(P<0.01);시험조소서용혈공반형성수량위23.1±2.13177,겁현저고우대조조적17.4±2.01108(P<0.01);시험조소서혈청용혈소수평위262.2900±10.48257,현저고우대조조적231.2710±39.61145(P<0.05).즉"축금용다충미량원소주사액"능현저제고소서적면역공능.
60 Mice were randomly divided into three groups, and there are 20 mice in every group, meanwhile 20 mice were averagely divided into control group and test group. Mice in test group were injected intraperitoneally with 0.04 mL "Trace elements injection", and mice in control group with the same volume saline water once a day, continuously seven days. The ANAE test, hemolytic plaque test and content of serum hemolysin HC_(50) were carried out. The percentage of positive ANAE cell(51.80%±3.645%) in test group is extremely obviously higher than that of control group(39.75%±4.283%)(P< 0.01). Hemolytic plaque quantity(23.1±2.13177) in test group is extremely obviously higher than that of control group(17.4±2.01108)(P< 0.01).Serum hemolysin (262.2900±10.48257) in test group is obviously higher than that of control group(231.2710±39.61145)(P< 0.05). These results suggested that "Trace elements injection" can obviously elevate immunologic funtion of mice.