中国医师进修杂志
中國醫師進脩雜誌
중국의사진수잡지
CHINESE JOURNAL OF POSTGRADUATES OF MEDICINE
2010年
33期
10-13
,共4页
轮状病毒感染%抗体%免疫,天然
輪狀病毒感染%抗體%免疫,天然
륜상병독감염%항체%면역,천연
Rotavirus infections%Antibodies%Immunity,natural
目的 通过对轮状病毒腹泻小儿特异性体液免疫应答的研究,为有效预防和治疗轮状病毒感染提供理论依据.方法 75例粪轮状病毒检测阳性患儿(腹泻组)及45例近期无腹泻和其他感染性疾病小儿(对照组),采用ELISA法检测抗原及血浆和粪标本中的特异性抗体;采用反转录PCR法确定轮状病毒基因型;采用荧光定量PCR法进行外周血单个核细胞细胞因子mRNA表达的检测.结果 腹泻组轮状病毒G分型以G3型(77.3%,58/75)为主,P分型以P8型(82.7%,62/75)为主;腹泻组血浆中特异性抗体滴度均明显高于对照组,粪标本中IgA抗体滴度的增高尤为显著;腹泻组患儿CD19+细胞比率为(30.8±7.9)%,显著高于对照组的(23.1±7.7)%(P=0.009).CD4+细胞比率明显下降(P=0.005),IL-12p40 mRNA的表达水平在疾病全程均比对照组明显升高(P＜0.01).结论 轮状病毒感染小儿免疫应答以特异性体液免疫,尤其是黏膜免疫的显著增强为主要特点.
目的 通過對輪狀病毒腹瀉小兒特異性體液免疫應答的研究,為有效預防和治療輪狀病毒感染提供理論依據.方法 75例糞輪狀病毒檢測暘性患兒(腹瀉組)及45例近期無腹瀉和其他感染性疾病小兒(對照組),採用ELISA法檢測抗原及血漿和糞標本中的特異性抗體;採用反轉錄PCR法確定輪狀病毒基因型;採用熒光定量PCR法進行外週血單箇覈細胞細胞因子mRNA錶達的檢測.結果 腹瀉組輪狀病毒G分型以G3型(77.3%,58/75)為主,P分型以P8型(82.7%,62/75)為主;腹瀉組血漿中特異性抗體滴度均明顯高于對照組,糞標本中IgA抗體滴度的增高尤為顯著;腹瀉組患兒CD19+細胞比率為(30.8±7.9)%,顯著高于對照組的(23.1±7.7)%(P=0.009).CD4+細胞比率明顯下降(P=0.005),IL-12p40 mRNA的錶達水平在疾病全程均比對照組明顯升高(P＜0.01).結論 輪狀病毒感染小兒免疫應答以特異性體液免疫,尤其是黏膜免疫的顯著增彊為主要特點.
목적 통과대륜상병독복사소인특이성체액면역응답적연구,위유효예방화치료륜상병독감염제공이론의거.방법 75례분륜상병독검측양성환인(복사조)급45례근기무복사화기타감염성질병소인(대조조),채용ELISA법검측항원급혈장화분표본중적특이성항체;채용반전록PCR법학정륜상병독기인형;채용형광정량PCR법진행외주혈단개핵세포세포인자mRNA표체적검측.결과 복사조륜상병독G분형이G3형(77.3%,58/75)위주,P분형이P8형(82.7%,62/75)위주;복사조혈장중특이성항체적도균명현고우대조조,분표본중IgA항체적도적증고우위현저;복사조환인CD19+세포비솔위(30.8±7.9)%,현저고우대조조적(23.1±7.7)%(P=0.009).CD4+세포비솔명현하강(P=0.005),IL-12p40 mRNA적표체수평재질병전정균비대조조명현승고(P＜0.01).결론 륜상병독감염소인면역응답이특이성체액면역,우기시점막면역적현저증강위주요특점.
Objective To apply the theory basis for effectively preventing and treating rotavirus infection by studying on specific humoral immune response in children with rotavirus diarrhea. Methods Seventy-five patients of children (diarrhea group ) and 45 controls (control group) were selected. The antigen and specific antibody in plasma and stool were detected by ELISA method;the type of viral gene was confirmed by reverse transcription PCR method;the expression of mRNA in peripheral blood mononuclear cell was measured by fluorescent light quantitation PCR method. Results In diarrhea group, rotavirus G typing to G3 dominated, accounting for 77.3% (58/75), P sub-type to P8 mainly, accounting for 82.7% (62/75). Rotavirus-specific antibody titers of plasma in diarrhea group were significantly higher than those in control group, IgA antibody titers of stool increased significantly, not only higher than that in control group and plasma. The percentage of CD19+ cells in diarrhea group [(30.8 ± 7.9)%] was significantly higher than that in control group [(23.1 ± 7.7 )%] (P = 0.009 ). And the proportion of CD4+ cells decreased significantly (P = 0.005 ), the expression level of IL- 12p40 mRNA in the entire process in diarrhea group was significantly higher than that in control group(P＜ 0.01 ). Conclusion Children with acute rotavirus infection early in the immune response to specific humoral immunity, especially mucosal immune to significantly improve the main features.
