中国综合临床
中國綜閤臨床
중국종합림상
CLINICAL MEDICINE OF CHINA
2012年
10期
1034-1038
,共5页
邹艳慧%高学文%王萍%云雅丽
鄒豔慧%高學文%王萍%雲雅麗
추염혜%고학문%왕평%운아려
小肠脂肪酸结合蛋白基因多态性%血脂代谢异常%冠心病
小腸脂肪痠結閤蛋白基因多態性%血脂代謝異常%冠心病
소장지방산결합단백기인다태성%혈지대사이상%관심병
Intestinal fatty acid binding protein gene polymorphism%Dyslipidemia%Coronary heart disease
目的 探讨小肠型脂肪酸结合蛋白(FABP2)外显子2第54位密码子的基因多态性与不同民族冠心病患者血脂水平的关系.方法 收集入选人群的空腹外周血标本,全血用于提取DNA,血清用于检测血脂.采用聚合酶链反应(PCR),DNA限制性内切酶酶切(RFLP)等技术,分别对病例组(汉族冠心病组60例,蒙古族冠心病组60例)和对照组(汉族对照组51例,蒙古族对照组51例)54A/T FABP2 基因型进行分析.结果 (1)汉族冠心病组人群54T等位基因频率为0.542,54A等位基因频率为0.458;蒙古族冠心病人群54T等位基因频率为0.708,54A等位基因频率为0.292;汉族对照组人群54T等位基因频率为0.284,54A等位基因频率为0.716;蒙古族对照组人群54T等位基因频率为0.353,54A等位基因频率为0.647;与对照组相比:汉族及蒙古族冠心病组人群突变型54T等位基因频率明显增高,且差异均有统计学意义(统计值分别为:x2=14.967,P＜0.05;x2=28.083,P＜0.05);蒙古族冠心病人群突变型54T等位基因频率较汉族冠心病人群增高,且差异有统计学意义(x2=7.111,P＜0.05).(2)与FABP2 Thr54(-)者相比,冠心病组FABP2Thr54(+)者的空腹血浆TG[汉族冠心病:Thr54(-)人群为(1.89 ±0.57) mmol/L,Thr54(+)人群为(3.92±1.63) mmol/L,P=0.001;蒙古族冠心病组:Thr54 (-)人群为(2.23 ±0.13) mmol/L,Thr54(+)人群为(4.03±1.14)mmol/L,P=0.035]、低密度脂蛋白胆固醇[汉族冠心病:Thr54(-)人群为(3.09±0.92) mmol/L,Thr54(+)人群为(4.05±1.14) mmol/L,P=0.025;蒙古族冠心病组:Thr54(-)人群为(4.26±0.08) mmol/L,Thr54(+)人群为(5.10 ±0.56)mmol/L,P=0.045]水平升高.结论 (1)内蒙古自治区呼和浩特市市区蒙古族及汉族人群中存在FABP2外显子2第54位密码子的基因多态性.(2)FABP2基因多态性是冠心病患者脂质代谢异常的影响因素,可能与冠心病发病风险有关,携带FABP2 Thr基因的蒙古族个体冠心病发病风险增高.
目的 探討小腸型脂肪痠結閤蛋白(FABP2)外顯子2第54位密碼子的基因多態性與不同民族冠心病患者血脂水平的關繫.方法 收集入選人群的空腹外週血標本,全血用于提取DNA,血清用于檢測血脂.採用聚閤酶鏈反應(PCR),DNA限製性內切酶酶切(RFLP)等技術,分彆對病例組(漢族冠心病組60例,矇古族冠心病組60例)和對照組(漢族對照組51例,矇古族對照組51例)54A/T FABP2 基因型進行分析.結果 (1)漢族冠心病組人群54T等位基因頻率為0.542,54A等位基因頻率為0.458;矇古族冠心病人群54T等位基因頻率為0.708,54A等位基因頻率為0.292;漢族對照組人群54T等位基因頻率為0.284,54A等位基因頻率為0.716;矇古族對照組人群54T等位基因頻率為0.353,54A等位基因頻率為0.647;與對照組相比:漢族及矇古族冠心病組人群突變型54T等位基因頻率明顯增高,且差異均有統計學意義(統計值分彆為:x2=14.967,P＜0.05;x2=28.083,P＜0.05);矇古族冠心病人群突變型54T等位基因頻率較漢族冠心病人群增高,且差異有統計學意義(x2=7.111,P＜0.05).(2)與FABP2 Thr54(-)者相比,冠心病組FABP2Thr54(+)者的空腹血漿TG[漢族冠心病:Thr54(-)人群為(1.89 ±0.57) mmol/L,Thr54(+)人群為(3.92±1.63) mmol/L,P=0.001;矇古族冠心病組:Thr54 (-)人群為(2.23 ±0.13) mmol/L,Thr54(+)人群為(4.03±1.14)mmol/L,P=0.035]、低密度脂蛋白膽固醇[漢族冠心病:Thr54(-)人群為(3.09±0.92) mmol/L,Thr54(+)人群為(4.05±1.14) mmol/L,P=0.025;矇古族冠心病組:Thr54(-)人群為(4.26±0.08) mmol/L,Thr54(+)人群為(5.10 ±0.56)mmol/L,P=0.045]水平升高.結論 (1)內矇古自治區呼和浩特市市區矇古族及漢族人群中存在FABP2外顯子2第54位密碼子的基因多態性.(2)FABP2基因多態性是冠心病患者脂質代謝異常的影響因素,可能與冠心病髮病風險有關,攜帶FABP2 Thr基因的矇古族箇體冠心病髮病風險增高.
목적 탐토소장형지방산결합단백(FABP2)외현자2제54위밀마자적기인다태성여불동민족관심병환자혈지수평적관계.방법 수집입선인군적공복외주혈표본,전혈용우제취DNA,혈청용우검측혈지.채용취합매련반응(PCR),DNA한제성내절매매절(RFLP)등기술,분별대병례조(한족관심병조60례,몽고족관심병조60례)화대조조(한족대조조51례,몽고족대조조51례)54A/T FABP2 기인형진행분석.결과 (1)한족관심병조인군54T등위기인빈솔위0.542,54A등위기인빈솔위0.458;몽고족관심병인군54T등위기인빈솔위0.708,54A등위기인빈솔위0.292;한족대조조인군54T등위기인빈솔위0.284,54A등위기인빈솔위0.716;몽고족대조조인군54T등위기인빈솔위0.353,54A등위기인빈솔위0.647;여대조조상비:한족급몽고족관심병조인군돌변형54T등위기인빈솔명현증고,차차이균유통계학의의(통계치분별위:x2=14.967,P＜0.05;x2=28.083,P＜0.05);몽고족관심병인군돌변형54T등위기인빈솔교한족관심병인군증고,차차이유통계학의의(x2=7.111,P＜0.05).(2)여FABP2 Thr54(-)자상비,관심병조FABP2Thr54(+)자적공복혈장TG[한족관심병:Thr54(-)인군위(1.89 ±0.57) mmol/L,Thr54(+)인군위(3.92±1.63) mmol/L,P=0.001;몽고족관심병조:Thr54 (-)인군위(2.23 ±0.13) mmol/L,Thr54(+)인군위(4.03±1.14)mmol/L,P=0.035]、저밀도지단백담고순[한족관심병:Thr54(-)인군위(3.09±0.92) mmol/L,Thr54(+)인군위(4.05±1.14) mmol/L,P=0.025;몽고족관심병조:Thr54(-)인군위(4.26±0.08) mmol/L,Thr54(+)인군위(5.10 ±0.56)mmol/L,P=0.045]수평승고.결론 (1)내몽고자치구호화호특시시구몽고족급한족인군중존재FABP2외현자2제54위밀마자적기인다태성.(2)FABP2기인다태성시관심병환자지질대사이상적영향인소,가능여관심병발병풍험유관,휴대FABP2 Thr기인적몽고족개체관심병발병풍험증고.
Objective To explore the relationship between FABP2 54 gene polymorphism and serum lipid levels in different ethnic patients with coronary heart disease.Methods We collected fasting blood from participants,then extracted DNA from whole blood,and detected lipids from serum.We performed 54A/T FABP2 genotyping for participants in the case and the control groups by polymerase chain reaction (PCR) and DNA restriction enzyme digestion techniques.Results ( 1 ) In Hans population with coronary heart disease,the allelic frequency of point in 54T gene was 0.542,and 54A gene was 0.458 ; in urban Mongolian with coronary heart disease,the allelic frequency of point in 54T gene was 0.708,and that of 54A gene was 0.292.The allelic frequency of point in 54T and 54A genes were 0.284 and 0.716 respectively in the Han population control group and 0.353 and 0.647 respectively in the normal urban Mongolian group.Compared with the control groups,mutant 54T allele frequency in the case groups of Han and urban Mongolian was significantly higher ( Han:x2 =14.967,P ＜ 0.05 ; Mongolian:x2 =28.083,P ＜ 0.05 ).Compared with the Han Chinese population with coronary heart disease,the mutant 54T allele frequency in urban Mongolian with coronary heart disease was significantly (x2 =7.111,P ＜ 0.05 ).There was no significant difference in mutant 54T allele frequency between the Mongolian control group and the Han control group ( x2 =3.392,P ＞ 0.05 ).( 2 ) Patients with coronary heart disease who carry Thr54 ( + ) had a significantly higher level of fasting plasma TG [ CC Thr54 (-):( 1.89 ± 0.57 ) mmol/L,CC Thr54 ( + ):( 3.92 ± 1.63 ) mmol/L; CM Thr54 (-):(2.23 ± 0.13 ) mmol/L,CM Thr54 ( + ):(4.03 ± 1.14) mmol/L; Hans:P =0.001,Mongolian:P =0.035 ],and LDL-C [ CC Thr54 (-):( 3.09 ± 0.92) mmol/L,CC Thr54 ( + ):(4.05 ± 1.14 ) mmol/L ; CM Thr54 (-):( 4.26 ± 0.08 ) mmol/L,CM Thr54 ( + ):( 5.10 ± 0.56 ) mmol/L; Hans:P =0.025,Mongolian:P =0.045 ] than those with Thr54 (-).Conclusion (1) Threre is FABP2 gene polymorphism in urban Mongolian and Han population.Gene mutation frequency is in accordance with Hardy-Weinberg law of genetic equilibrium,suggesting the sample group representative.(2) FABP2 polymorphism may have a certain contribution to dyslipidemia in patients with coronary heart disease.FABP2 gene polymorphism may be associated with coronary heart disease.Individuals of Mongolian with FABP2 Thr54( + ) has a higher risk of coronary heart disease.
