中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2009年
7期
892-895
,共4页
川芎/药理学%地龙/药理学%黄芪/药理学%内皮,血管/药物作用%脑缺血/中药疗法%再灌注损伤/预防和控制%细胞凋亡
川芎/藥理學%地龍/藥理學%黃芪/藥理學%內皮,血管/藥物作用%腦缺血/中藥療法%再灌註損傷/預防和控製%細胞凋亡
천궁/약이학%지룡/약이학%황기/약이학%내피,혈관/약물작용%뇌결혈/중약요법%재관주손상/예방화공제%세포조망
Ligustieum ehuanxiong/PD%Lumbricus/PD%Astragalus membranaceus/PD%Endothelium,vascular/DE%Brain ische-mia/ZD%Reperfusion injury/PC%Apoptosis
目的 从凋亡信号通路探讨中药保护脑缺血再灌注损伤中血管内皮细胞凋亡机制.方法 将64只清洁级SD大鼠随机分为4组(每组16只):假手术对照组、脑缺血再灌注组、脑缺血预处理组和中药预处理组;再将脑缺血再灌注组、脑缺血预处理组和中药预处理组按不同时相分为1、7、14及21 d的4个亚组,中药预处理组应用中药脑泰方提取物进行干预.取大鼠大脑中静脉血管内皮细胞,分别行Hoechst 33258染色后计数以及DNA"梯状"条带检测内皮细胞凋亡情况,再应用Western-blot分析中药脑泰方对Caspase-3,8,9活化和Bid裂解的影响情况;从凋亡信号通路探讨中药保护脑缺血再灌注损伤中血管内皮细胞凋亡机制.结果 中药脑泰方提取物可抑制脑缺血再灌注损伤中血管内皮细胞凋亡,能明显抑制缺血再灌注损伤所致的Caspase-3,8,9活化,也抑制了Bid裂解成tBid.结论 中药脑泰方提取物从凋亡信号通路抑制了脑缺血再灌注损伤中血管内皮细胞凋亡.
目的 從凋亡信號通路探討中藥保護腦缺血再灌註損傷中血管內皮細胞凋亡機製.方法 將64隻清潔級SD大鼠隨機分為4組(每組16隻):假手術對照組、腦缺血再灌註組、腦缺血預處理組和中藥預處理組;再將腦缺血再灌註組、腦缺血預處理組和中藥預處理組按不同時相分為1、7、14及21 d的4箇亞組,中藥預處理組應用中藥腦泰方提取物進行榦預.取大鼠大腦中靜脈血管內皮細胞,分彆行Hoechst 33258染色後計數以及DNA"梯狀"條帶檢測內皮細胞凋亡情況,再應用Western-blot分析中藥腦泰方對Caspase-3,8,9活化和Bid裂解的影響情況;從凋亡信號通路探討中藥保護腦缺血再灌註損傷中血管內皮細胞凋亡機製.結果 中藥腦泰方提取物可抑製腦缺血再灌註損傷中血管內皮細胞凋亡,能明顯抑製缺血再灌註損傷所緻的Caspase-3,8,9活化,也抑製瞭Bid裂解成tBid.結論 中藥腦泰方提取物從凋亡信號通路抑製瞭腦缺血再灌註損傷中血管內皮細胞凋亡.
목적 종조망신호통로탐토중약보호뇌결혈재관주손상중혈관내피세포조망궤제.방법 장64지청길급SD대서수궤분위4조(매조16지):가수술대조조、뇌결혈재관주조、뇌결혈예처리조화중약예처리조;재장뇌결혈재관주조、뇌결혈예처리조화중약예처리조안불동시상분위1、7、14급21 d적4개아조,중약예처리조응용중약뇌태방제취물진행간예.취대서대뇌중정맥혈관내피세포,분별행Hoechst 33258염색후계수이급DNA"제상"조대검측내피세포조망정황,재응용Western-blot분석중약뇌태방대Caspase-3,8,9활화화Bid렬해적영향정황;종조망신호통로탐토중약보호뇌결혈재관주손상중혈관내피세포조망궤제.결과 중약뇌태방제취물가억제뇌결혈재관주손상중혈관내피세포조망,능명현억제결혈재관주손상소치적Caspase-3,8,9활화,야억제료Bid렬해성tBid.결론 중약뇌태방제취물종조망신호통로억제료뇌결혈재관주손상중혈관내피세포조망.
Objective To investigate the mechanisms of Chinese traditional medicine mixture protection of vascular endothelial cell from apoptosis in cerebral ischemia-reperfusion injury. Methods Healthy, clean SD rats were randomly divided into 4 groups: Sham opera-tion group (SOG), cerebral ischemia reperfusion injury group (IRG), cerebral ischemia preconditioning group (IPG) and Chinese tradi-tional medicine mixture preconditioning group (CPG). Furthermore, IRG, IPG and NPC were divided into 4 sub-groups: 1 d, 7d, 14d, 21d subgroup, according to the different time point since ischemia-reperfusion took place. And in CPG, Naotai formula extract was used. Cere-bral vascular endothelial cells of rats were removed and Hoechst 33258 staining and the DNA gradient bands were used to detect the apoptosis of these cells. Then the influence of Naotai formula extract on caspase-3, 8 and 9 activation, and Bid lysis was examined by Western-blot, and the mechanisms of Chinese traditional medicine mixture protection of vascular endothelial cell were investigated from apoptosis signal pathway. Result Naotai formula extract can inhibit the apoptosis of endothelial cells in ischemia-reperfusion injury, and it also can inhibit the activation of caspase-3, 8 and 9, thus inhibit the lysis of Bid into tBid. Conclusion Naomi formula extract inhibit the apoptosis of endo-thelial ceils in ischemia-reperfusion injury via apoptosis signal pathway.
