CAJ | 학술논문

目的研究125碘 - 辣根过氧化物酶（125I - HRP）追踪术后神经轴浆流的可行性;为临床应用放射性同位素研究神经再生提供实验依据。方法 24只SD大鼠,按手术先后顺序分为两组。(1) 实验组: 于坐骨结节远端0.8 cm处切断大鼠右侧坐骨神经后立即行端端缝合术。术后4周,从右侧小腿三头肌内注射125I - HRP。(2) 对照组:将125I - HRP注射到大鼠右侧小腿三头肌内。于注射125I - HRP 24 h后,实验组取神经缝合口近段0.8 cm神经干,对照组取右侧坐骨结节远端的坐骨神经0.8 cm;两组同时切取该段神经周围肌肉和左侧相应部位坐骨神经干,进行125碘放射性强度的г - 计数。结果对照组,注射侧坐骨神经干的125碘放射性强度是其它组织的30倍以上。实验组,注射侧坐骨神经的125碘放射性强度是其周围肌肉和对侧坐骨神经的6倍和7倍。两组注射侧坐骨神经的125碘放射性强度和其它组织相比,差异均有显著性意义(P ＜ 0.01)。结论 125I - HRP注入肌肉被神经末梢摄取后,经轴浆逆流可以通过神经缝合口到达神经近段;应用г - 计数器可探测神经组织中的125碘放射性强度。
목적연구125전 - 랄근과양화물매（125I - HRP）추종술후신경축장류적가행성;위림상응용방사성동위소연구신경재생제공실험의거。방법 24지SD대서,안수술선후순서분위량조。(1) 실험조: 우좌골결절원단0.8 cm처절단대서우측좌골신경후립즉행단단봉합술。술후4주,종우측소퇴삼두기내주사125I - HRP。(2) 대조조:장125I - HRP주사도대서우측소퇴삼두기내。우주사125I - HRP 24 h후,실험조취신경봉합구근단0.8 cm신경간,대조조취우측좌골결절원단적좌골신경0.8 cm;량조동시절취해단신경주위기육화좌측상응부위좌골신경간,진행125전방사성강도적г - 계수。결과대조조,주사측좌골신경간적125전방사성강도시기타조직적30배이상。실험조,주사측좌골신경적125전방사성강도시기주위기육화대측좌골신경적6배화7배。량조주사측좌골신경적125전방사성강도화기타조직상비,차이균유현저성의의(P ＜ 0.01)。결론 125I - HRP주입기육피신경말소섭취후,경축장역류가이통과신경봉합구도체신경근단;응용г - 계수기가탐측신경조직중적125전방사성강도。
Objective　To study the feasibility of tracing axoplasmic flow by 125Iodine horseradish paroxidase (125I-HRP) in order to provide the experimental basis for study of nerve regeneration using radioisotope. Methods　Twenty - four SD rats were divided into control group and experimental group. In the experimental group, the right sciatic nerve of the rat was transected at the site 0.8 cm distal to the sciatic notch and then sutured immediately. Four weeks later, 125I - HRP was injected into the right soleus muscles. In the control group, Only injection of 125I - HRP into the right soleus muscles was done. 24 hours after HRP injection, sciatic nerve segment 0.8 cm in length proximal to the suture site of the experimental group and distal to the sciatic notch in the control group were harvested respectively. The muscle around the nerve and the left sciatic nerve segment at the corresponding level were also harvested. 125Iodine counting was done.　Results　The intensity of radioactivity of 125Iodine in the sciatic nerve of the injection side in the control group was 30 times of that in other tissues. In the experimental group, the 125Iodine radioactivity of the sciatic nerve of the injection side was 6 times of that in the muscles around the nerve and 7 times of that in the left sciatic nerve. The difference was statistically significant.　Conclusions　125I - HRP injected into the muscle can be absorbed by nerve endings and be transported by retrograde axoplasmic flow to pass through the anastomosis site and reach the proximal nerve segment. The intensity of radioactivity of 125Iodinein in nerve tissue can be detected by г -counting.