CAJ | 학술논문

目的探讨人重组促红细胞生成素(rh-EPO)对大鼠坐骨神经断裂后神经再生的作用.方法选用健康雄性Wistar大鼠36只,制备大鼠左侧坐骨神经修复模型.实验动物随机分为2组,每组18只,EPO组:腹腔注射rh-EPO 3 000 U/kg;对照组:注射同体积的生理盐水.术后第4周、8周分别进行坐骨神经功能指数(SFI)、生物力学检测、组织学观察、电生理检测、有髓纤维密度密度测定、有髓纤维截面积测定.结果术后第4周,EPO组和对照组SFI分别为-65.26±3.42和-70.83±4.12,最大抗牵拉强度分别为(3.86±0.29)N/mm2和(3.38±0.21)N/mm2,运动神经潜伏期延迟比分别为2.34±0.23和2.78±0.29,运动神经波幅恢复比分别为0.23±0.05和0.14±0.03;术后第8周,EPO组和对照组SFI分别为-51.34±2.98和-57.23±4.86,最大抗牵拉强度分别为(4.67±0.36)N/mm2和(4.13±0.32)N/mm2,运动神经潜伏期延迟比分别为1.32±0.15和1.62±0.21,运动神经波幅恢复比分别为0.41±0.09和0.26±0.07,神经纤维通过比分别为0.57±0.05和0.38±0.03,有髓纤维截面积恢复比分别为0.81±0.06和0.58±0.03,两组之间差异均有统计学意义(P＜0.05),EPO组均优于对照组.结论 rh-EPO能促进坐骨神经再生和功能恢复.
목적 탐토인중조촉홍세포생성소(rh-EPO)대대서좌골신경단렬후신경재생적작용.방법 선용건강웅성Wistar대서36지,제비대서좌측좌골신경수복모형.실험동물수궤분위2조,매조18지,EPO조:복강주사rh-EPO 3 000 U/kg;대조조:주사동체적적생리염수.술후제4주、8주분별진행좌골신경공능지수(SFI)、생물역학검측、조직학관찰、전생리검측、유수섬유밀도밀도측정、유수섬유절면적측정.결과 술후제4주,EPO조화대조조SFI분별위-65.26±3.42화-70.83±4.12,최대항견랍강도분별위(3.86±0.29)N/mm2화(3.38±0.21)N/mm2,운동신경잠복기연지비분별위2.34±0.23화2.78±0.29,운동신경파폭회복비분별위0.23±0.05화0.14±0.03;술후제8주,EPO조화대조조SFI분별위-51.34±2.98화-57.23±4.86,최대항견랍강도분별위(4.67±0.36)N/mm2화(4.13±0.32)N/mm2,운동신경잠복기연지비분별위1.32±0.15화1.62±0.21,운동신경파폭회복비분별위0.41±0.09화0.26±0.07,신경섬유통과비분별위0.57±0.05화0.38±0.03,유수섬유절면적회복비분별위0.81±0.06화0.58±0.03,량조지간차이균유통계학의의(P＜0.05),EPO조균우우대조조.결론 rh-EPO능촉진좌골신경재생화공능회복.
Objective To investigate the effect of recombinant human erythropoietin(rh-EPO) on the nerve regeneration of adult rats sciatic nerves. Methods Tirty-six healthy male Wistar rats were involved and left sciatic nerve repaired model was used.The experimental rats were divided randomly into two groups:the EPO group and the control group,18 rats in each group.rh-EPO 3 000 U/kg was injected daily into the abdominal in EPO group,and normal saline was injected into the abdominal every day after operation in control group.On 4 and 8 weeks after operation,these items were determined,the sciatic function index (SFI),biomechanics examination,histological observation,electrophysiological examination,myelinated fibers density and sectional area measurement.Results On weeks 4 after operation,the SFI of EPO group and control group were-65.26 ± 3.42 and-70.83 ± 4.12,respectively,the maximum tensile resistance were (3.86 ± 0.29)N/mm2 and (3.38 ± 0.21 )N/mm2,the delayed ratio of latency of motor nerve were 2.34 ± 0.23 and 2.78 ± 0.29,and the recovery ratio of wave amplitude were 0.23 ± 0.05 and 0.14 ± 0.03 respectively.On eight weeks after operation,the SFI of EPO group and control group were-51.34 ± 2.98 and-57.23 ± 4.86,respcetively,the maximum tensile resistance were (4.67 ± 0.36) N/mm2 and (4.13 ± 0.32) N/mm2,the delayed ratio of latency of motor nerve were 1.32 ± 0.15 and 1.62 ± 0.21,the recovery ratio of wave amplitude were 0.41 ± 0.09 and 0.26 ± 0.07,the nerve fibers cross ratio were 0.57 ± 0.05 and 0.38 ± 0.03,and the recovery ratio of sectional area of myelinated fibers were 0.81 ± 0.06 and 0.58 ± 0.03,respectively.Those items in EPO group were significantly superior to those in the control group (P ＜ 0.05 ＝.Conclusion rh-EPO can promote the injured nerve regeneration and improve the recovery of their function.