中华肿瘤杂志
中華腫瘤雜誌
중화종류잡지
CHINESE JOURNAL OF ONCOLOGY
2010年
7期
533-537
,共5页
罗春芳%洪慧莉%芦玉兰%王晖%刘铭球
囉春芳%洪慧莉%蘆玉蘭%王暉%劉銘毬
라춘방%홍혜리%호옥란%왕휘%류명구
宫颈肿瘤%碱性成纤维细胞生长因子%第10号染色体缺失性磷酸酶-张力蛋白同源物基因
宮頸腫瘤%堿性成纖維細胞生長因子%第10號染色體缺失性燐痠酶-張力蛋白同源物基因
궁경종류%감성성섬유세포생장인자%제10호염색체결실성린산매-장력단백동원물기인
Uterine cervical neoplasms%Basic fibroblast growth factor%Phosphatase and tensin homologue deleted on chromosome 10
目的 研究碱性成纤维细胞生长因子(bFGF)和第10号染色体缺失性磷酸酶-张力蛋白同源物基因(PTEN)在宫颈癌组织中的表达及临床意义.方法 采用组织芯片和免疫组化技术检测143例宫颈浸润癌(ICC)和20例癌旁正常官颈上皮(NCE)中bFGF和PTEN的表达,分析bFGF和PTEN表达与病理分级、临床分期和淋巴结转移等临床病理因素的关系.采用SPSS 13.0统计软件包进行统计学分析,相关性分析采用Spearman等级相关分析.结果 bFGF在ICC和NCE中的阳性表达率分别为88.8%(127/143)和25.0%(5/20,P=0.000);PTEN在ICC和NCE中的阳性表达率分别为67.1%(96/143)和100.0%(20/20,P=0.000).bFGF的表达与淋巴结转移(r=0.239,P=0.004)和病理分级(r=0.369,P=0.000)呈正相关,PTEN的表达与临床分期(r=-0.189,P=0.024)、病理分级(r=-0.211,P=0.011)和淋巴结转移(r=-0.321,P=0.000)呈负相关.bFGF和PTEN在ICC中的表达强度呈负相关(r=0.261,P=0.002).随着ICC恶性程度增高,PTEN的表达强度逐渐下降,bFGF的表达则呈上升趋势.结论 bFGF和PTEN在ICC中的异常表达与肿瘤的分化、侵袭和发展有关,联合检测bFGF和PTEN可用于评估ICC的生物学行为.
目的 研究堿性成纖維細胞生長因子(bFGF)和第10號染色體缺失性燐痠酶-張力蛋白同源物基因(PTEN)在宮頸癌組織中的錶達及臨床意義.方法 採用組織芯片和免疫組化技術檢測143例宮頸浸潤癌(ICC)和20例癌徬正常官頸上皮(NCE)中bFGF和PTEN的錶達,分析bFGF和PTEN錶達與病理分級、臨床分期和淋巴結轉移等臨床病理因素的關繫.採用SPSS 13.0統計軟件包進行統計學分析,相關性分析採用Spearman等級相關分析.結果 bFGF在ICC和NCE中的暘性錶達率分彆為88.8%(127/143)和25.0%(5/20,P=0.000);PTEN在ICC和NCE中的暘性錶達率分彆為67.1%(96/143)和100.0%(20/20,P=0.000).bFGF的錶達與淋巴結轉移(r=0.239,P=0.004)和病理分級(r=0.369,P=0.000)呈正相關,PTEN的錶達與臨床分期(r=-0.189,P=0.024)、病理分級(r=-0.211,P=0.011)和淋巴結轉移(r=-0.321,P=0.000)呈負相關.bFGF和PTEN在ICC中的錶達彊度呈負相關(r=0.261,P=0.002).隨著ICC噁性程度增高,PTEN的錶達彊度逐漸下降,bFGF的錶達則呈上升趨勢.結論 bFGF和PTEN在ICC中的異常錶達與腫瘤的分化、侵襲和髮展有關,聯閤檢測bFGF和PTEN可用于評估ICC的生物學行為.
목적 연구감성성섬유세포생장인자(bFGF)화제10호염색체결실성린산매-장력단백동원물기인(PTEN)재궁경암조직중적표체급림상의의.방법 채용조직심편화면역조화기술검측143례궁경침윤암(ICC)화20례암방정상관경상피(NCE)중bFGF화PTEN적표체,분석bFGF화PTEN표체여병리분급、림상분기화림파결전이등림상병리인소적관계.채용SPSS 13.0통계연건포진행통계학분석,상관성분석채용Spearman등급상관분석.결과 bFGF재ICC화NCE중적양성표체솔분별위88.8%(127/143)화25.0%(5/20,P=0.000);PTEN재ICC화NCE중적양성표체솔분별위67.1%(96/143)화100.0%(20/20,P=0.000).bFGF적표체여림파결전이(r=0.239,P=0.004)화병리분급(r=0.369,P=0.000)정정상관,PTEN적표체여림상분기(r=-0.189,P=0.024)、병리분급(r=-0.211,P=0.011)화림파결전이(r=-0.321,P=0.000)정부상관.bFGF화PTEN재ICC중적표체강도정부상관(r=0.261,P=0.002).수착ICC악성정도증고,PTEN적표체강도축점하강,bFGF적표체칙정상승추세.결론 bFGF화PTEN재ICC중적이상표체여종류적분화、침습화발전유관,연합검측bFGF화PTEN가용우평고ICC적생물학행위.
Objective To investigate the expressions of bFGF and PTEN in cervical carcinoma and their clinical significance. Methods Tissue microarray technique and immunohistochemistry SP method were used to detect the expressions of bFGF and PTEN in 143 cases of invasive carcinoma of cervix ( ICC) and 20 cases of normal cervical epithelium remote from tumor ( NCE ). The relationship between the expressions of bFGF and PTEN in ICC and some factors relating to clinical pathology of cervical carcinoma such as histopathological grading, lymph node metastasis, stroma involvement and FIGO staging were analyzed. Results The rate of the positive expression of bFGF in ICC was significantly higher than that in NCE 88.8% (127/143) vs. 25.0% (5/20, P=0.000). The rate of positive expression of PTEN in ICC was significantly lower than that in NCE 67. 1% (96/143) vs. 100. 0% (20/20, P =0. 000). The expression of bFGF was positively correlated with lymph node metastasis and histopathological grading ( r = 0.239, P = 0. 004 and r = 0. 369, P = 0. 000, respectively). The expression of PTEN was negatively correlated with FIGO staging, histopathological grading and lymph node metastasis (r= -0.189, P= 0.024; r= -0.211,P =0.011; r= -0. 321 ,P =0. 000, respectively). The expression of bFGF was negatively correlated with the expression of PTEN in ICC (r = -0.261 ,P =0.002). Conclusion The overexpression of bFGF and underexpression of PTEN are closely related to the invasion and growth of cervical carcinoma. Detection of the expression of both bFGF and PTEN may be of value in further understanding the biological behavior and predicting the prognosis of cervical carcinoma.
