CAJ | 학술논문

目的研究脂联素在慢性乙型肝炎病毒感染患者中的表达,并对部分患者抗病毒治疗前后的结果进行对比,探讨脂联素与慢性乙型肝炎肝纤维化的相关性.方法采用酶联免疫法(ELISA)定量检测血清脂联素水平,免疫组织化学法检测肝活检组织脂联素的表达,同时检测患者肝功能以及HBV DNA变化,并对患者进行抗病毒治疗前后的纤维化程度进行比较.结果血清脂联素在肝纤维化的各个阶段均有表达且与肝纤维化的分级呈正相关(r=0.976,P＜0.01),与转氨酶水平及HBV DNA浓度无相关性(P＞0.05);血清脂联素与肝硬化呈独立相关(P=0.02,相对危险度为1.07,95%可信区间为1.00～1.14).在抗病毒治疗后,肝纤维化程度改善以及获得持续病毒学应答的患者血清脂联素水平降低.结论血清脂联素在慢性乙型肝炎肝纤维化的进展过程中发挥了作用,血清脂联素水平在抗病毒治疗后显著下降,并与肝纤维化改善有关.
목적 연구지련소재만성을형간염병독감염환자중적표체,병대부분환자항병독치료전후적결과진행대비,탐토지련소여만성을형간염간섬유화적상관성.방법 채용매련면역법(ELISA)정량검측혈청지련소수평,면역조직화학법검측간활검조직지련소적표체,동시검측환자간공능이급HBV DNA변화,병대환자진행항병독치료전후적섬유화정도진행비교.결과 혈청지련소재간섬유화적각개계단균유표체차여간섬유화적분급정정상관(r=0.976,P＜0.01),여전안매수평급HBV DNA농도무상관성(P＞0.05);혈청지련소여간경화정독립상관(P=0.02,상대위험도위1.07,95%가신구간위1.00～1.14).재항병독치료후,간섬유화정도개선이급획득지속병독학응답적환자혈청지련소수평강저.결론 혈청지련소재만성을형간염간섬유화적진전과정중발휘료작용,혈청지련소수평재항병독치료후현저하강,병여간섬유화개선유관.
Objective To explore the expression of adiponectin in the chronic hepatitis B patients and compare adiponectin expression before and after antiviral therapy.Methods Serum adiponectin was detected by ELISA,expression of adiponectin in liver tissue was detected by immunohistochemistry.The liver functions and HBV-DNA was also tested.Liver biopsy tissue was assessed by fibrosis stage.Results Serum adiponectin was detected in different fibrosis stage and associated with fibrosis stage,not with HBV-DNA and liver function(ALT).Serum adiponectin is independently associated with liver cirrhosis(P=0.02;relative risk:1.07;95% confidence interval 1.00～1.14).At the end of antiviral therapy,the expressions of adiponectin have declined in patients with improved fibrosis and sustained viral response.Conclusions Serum adiponecin may play a role in chronic hepatitis B fibosis,and the reduction of adiponectin level is associated with improved fibrosis after antiviral therapy.