CAJ | 학술논문

研究肾癌细胞株786- 0,RC- 2及肾透明细胞癌组织中肝细胞黏附分子(hepatocyte cell adhesion molecule,hepaCAM)和血管内皮生长因子(VEGF)mRNA表达及其与肾透明细胞癌侵袭转移的关系.应用逆转录聚合酶链反应(RT-PCR)检测786- 0、RC- 2、正常肾组织hepaCAM和VEGF mRNA表达,73例肾透明细胞癌组织及相应癌旁组织中hepaCAM mRNA表达,43例肾透明细胞癌组织及相应癌旁组织VEGF mRNA表达,并比较它们之间的差异性和相关性.与正常肾组织比较786- 0,RC- 2的hepaCAM mRNA显著降低(P＜0.05);VEGF mRNA显著升高(P＜0.05).肾透明细胞癌组织hepaCAM mRNA显著低于癌旁组织(P＜0.05);VEGF mRNA显著高于癌旁组织(P＜0.05).在肾透明细胞癌组织中临床Ⅰ+Ⅱ期和Ⅲ+Ⅳ期两组VEGF mRNA表达差异具有统计学意义(P＜0.05),hepaCAM与VEGF mRNA呈负相关(r＝-0.329,P＜0.05).提示hepaCAM基因缺失可能参与肾透明细胞癌侵袭转移,其机制可能与调节VFGF表达改变有关,hepaCAM有望成为一种新的肾癌基因治疗的靶分子.
연구신암세포주786- 0,RC- 2급신투명세포암조직중간세포점부분자(hepatocyte cell adhesion molecule,hepaCAM)화혈관내피생장인자(VEGF)mRNA표체급기여신투명세포암침습전이적관계.응용역전록취합매련반응(RT-PCR)검측786- 0、RC- 2、정상신조직hepaCAM화VEGF mRNA표체,73례신투명세포암조직급상응암방조직중hepaCAM mRNA표체,43례신투명세포암조직급상응암방조직VEGF mRNA표체,병비교타문지간적차이성화상관성.여정상신조직비교786- 0,RC- 2적hepaCAM mRNA현저강저(P＜0.05);VEGF mRNA현저승고(P＜0.05).신투명세포암조직hepaCAM mRNA현저저우암방조직(P＜0.05);VEGF mRNA현저고우암방조직(P＜0.05).재신투명세포암조직중림상Ⅰ+Ⅱ기화Ⅲ+Ⅳ기량조VEGF mRNA표체차이구유통계학의의(P＜0.05),hepaCAM여VEGF mRNA정부상관(r＝-0.329,P＜0.05).제시hepaCAM기인결실가능삼여신투명세포암침습전이,기궤제가능여조절VFGF표체개변유관,hepaCAM유망성위일충신적신암기인치료적파분자.
It was to investigate the expression of hepaCAM and VEGF mRNA in renal cancer cells 786- 0,RC- 2 ,renal cell carcinomas(RCC)and their relation to invasion and metastasis of renal cell carcinomas(RCC).The expression of hepaCAM and VEGF mRNA was determined in 786- 0,RC- 2,normal renal tissue,the expression of hepaCAM mRNA was determined in 73 cases of RCC and corresponding peripheral noncancerous tissue and the expression of VEGF mRNA was determined in 43 cases of RCC and corresponding peripheral noncancerous tissue by reverse transcription polymerase chain reaction(RT-PCR).The difference and corelation between each group were compared and analyzed.Results indicate that hepaCAM mRNA′s expression was lower in 786- 0,RC- 2 than in the normal tissue(P＜0.05),VEGF mRNA′s expression was higher in 786- 0,RC- 2 than that of in the normal tissue(P＜0.05).In RCC tissues hepaCAM mRNA′s expression was significantly lower than corresponding peripheral noncancerous tissues(P＜0.05),VEGF mRNA′s expression was significantly higher than corresponding peripheral noncancerous tissues.There are significance between stage Ⅰ+Ⅱgroup and stageⅢ+Ⅳ group as far as VEGF mRNA expression are concerned(P＜0.05)and hepaCAM mRNA expression level was negatively correlated with the VEGF mRNA in RCC(r =-0.329,P＜0.05).Therefore,the down regulation of hepaCAM contributes to the invasiveness and metastasis of RCC,and the mechanism may be associated with the change of VEGF expression.hepaCAM is expected to become a new molecular in the gene therapy of RCC.