CAJ | 학술논문

目的:观察密蒙花提取物滴眼剂对去势所致干眼症雄鼠基础泪液分泌量、泪膜稳定性、泪腺中雄激素受体表达的影响,探讨密蒙花提取物滴眼剂抗雄激素水平下降所致干眼症的作用机制.方法:将45只Wister雄性大鼠随机分为空白组(A1、A2、A3)、模型组(B1、B2、B3)、密蒙花提取物滴眼剂治疗组(C1、C2、C3),共9组,1代表饲养1mo,2代表饲养2mo,3代表饲养3mo,每组5只,对B、C组行去势术建立动物模型,对C组以密蒙花提取物滴眼剂连续滴眼治疗1mo,对全部实验大鼠行Schirmer I试验、测量泪膜破裂时间,采用流式细胞仪检测泪腺中雄激素受体的表达.结果:C组Schirmer I试验测量值明显高于B组(P<0.01),泪膜破裂时间明显长于B组(P<0.01),显示密蒙花提取物滴眼剂能够显著维持泪腺基础分泌量和泪膜的稳定性;C组流式细胞仪检测的AR阳性表达率明显高于B组,显示密蒙花提取物滴眼剂中的有效成分可能有拟雄激素的效应.结论:密蒙花提取物滴眼剂中主要成分为黄酮类物质,可显著抑制雄激素水平降低后大鼠干眼症的发生,其作用机制可能与黄酮类物质结构与雄激素类似,可以起到拟雄激素效应,从而维持泪腺基础分泌量和泪膜的稳定性.
목적:관찰밀몽화제취물적안제대거세소치간안증웅서기출루액분비량、루막은정성、루선중웅격소수체표체적영향,탐토밀몽화제취물적안제항웅격소수평하강소치간안증적작용궤제.방법:장45지Wister웅성대서수궤분위공백조(A1、A2、A3)、모형조(B1、B2、B3)、밀몽화제취물적안제치료조(C1、C2、C3),공9조,1대표사양1mo,2대표사양2mo,3대표사양3mo,매조5지,대B、C조행거세술건립동물모형,대C조이밀몽화제취물적안제련속적안치료1mo,대전부실험대서행Schirmer I시험、측량루막파렬시간,채용류식세포의검측루선중웅격소수체적표체.결과:C조Schirmer I시험측량치명현고우B조(P<0.01),루막파렬시간명현장우B조(P<0.01),현시밀몽화제취물적안제능구현저유지루선기출분비량화루막적은정성;C조류식세포의검측적AR양성표체솔명현고우B조,현시밀몽화제취물적안제중적유효성분가능유의웅격소적효응.결론:밀몽화제취물적안제중주요성분위황동류물질,가현저억제웅격소수평강저후대서간안증적발생,기작용궤제가능여황동류물질결구여웅격소유사,가이기도의웅격소효응,종이유지루선기출분비량화루막적은정성.
AIM: To evaluate the effects of the extract of Buddleja officinalis eye drops in basic tears secretory volume, tear film stability, expression of androgen receptors(AR) in castrated rats with dry eye, and to investigate the therapeutic effects of extract of Buddleja officinalis on dry eye caused by gonadal hormones level imbalance. METHODS:A total of 45 Wistar masculinity rats were divided at random into 9 groups, including normal group(A1,A2 and A3), model group(B1,B2 and B3), therapy group with extract of Buddleja officinalis eye drops(C1,C2 and C3). The "1" stood for being fed for 1 month, and "2" for 2 months, and "3" for 3 months. The dry eye model was established with orchiectomy on group B,C. Group C was treated with Buddleja officinalis extract eye drops for one month. All rats were checked with Schirmer Ⅰ test (SⅠt) and tear film break-up time (BUT). Expression of AR was analyzed by flow cytometer(FCM). RESULTS:The SⅠt value of group C was significantly higher than that of group B (P<0.01) and the BUT value of group C was significantly longer than that of group B (P<0.01), which indicated the eye drop could significantly keep basic tears secretory volume and tear film stability. And the expression of AR of group C was much higher than that of group B,which showed that available composition of the eye drops maybe display androgen-like activity.CONCLUSION:The main components of extract of Buddleja officinalis is the flavonoids which could significantly inhibit happening of dry eye of rat after androgen level lowered. Its mechanism is like androgen's and it could display androgen-like activity to keep basic tears secretory volume and tear film stability.