国际肿瘤学杂志
國際腫瘤學雜誌
국제종류학잡지
JOURNAL OF INTERNATIONAL ONCOLOGY
2011年
6期
476-479
,共4页
结直肠肿瘤%疾病遗传易感性%多态性,单核苷酸
結直腸腫瘤%疾病遺傳易感性%多態性,單覈苷痠
결직장종류%질병유전역감성%다태성,단핵감산
Colorectal neoplasms%Genetic predisposition to disease%Polymorphism,single nucleotide
目的研究肽基精氨酸脱亚胺酶4(PADI4)基因与结直肠癌发病的相关性,进而探讨PADl4多态性在结直肠癌发病过程中的作用机制.方法采用病例-对照研究,用TaqMan-PCR法对515例结直肠癌患者和549例健康对照者的3个标签单核苷酸多态性(TagSNP)位点,即rs1886302、rs247713l和rs1635561进行基因分型,使用SHEsis和SPSS11.5软件对结果进行x2检验和Logistic回归分析.结果病例组与对照组关联性分析发现,位于PADI4基因内含子1区的rsl886302位点等位基因差异有统计学意义(P<0.05),基因型显性遗传模式差异也有统计学意义(P<0.05),等位基因A和基因型AA的OR值分别为0.796和0.731,携带等位基因A的个体会减小患结直肠癌的概率.而位于PADl4内含子1和内含子15区的rs2477131和rs1635561位点等位基因和基因型差异均无显著意义(P>0.05).结论PADl4基因是结直肠癌遗传易感基因,rsl886302是结直肠癌的易感性位点.
目的研究肽基精氨痠脫亞胺酶4(PADI4)基因與結直腸癌髮病的相關性,進而探討PADl4多態性在結直腸癌髮病過程中的作用機製.方法採用病例-對照研究,用TaqMan-PCR法對515例結直腸癌患者和549例健康對照者的3箇標籤單覈苷痠多態性(TagSNP)位點,即rs1886302、rs247713l和rs1635561進行基因分型,使用SHEsis和SPSS11.5軟件對結果進行x2檢驗和Logistic迴歸分析.結果病例組與對照組關聯性分析髮現,位于PADI4基因內含子1區的rsl886302位點等位基因差異有統計學意義(P<0.05),基因型顯性遺傳模式差異也有統計學意義(P<0.05),等位基因A和基因型AA的OR值分彆為0.796和0.731,攜帶等位基因A的箇體會減小患結直腸癌的概率.而位于PADl4內含子1和內含子15區的rs2477131和rs1635561位點等位基因和基因型差異均無顯著意義(P>0.05).結論PADl4基因是結直腸癌遺傳易感基因,rsl886302是結直腸癌的易感性位點.
목적연구태기정안산탈아알매4(PADI4)기인여결직장암발병적상관성,진이탐토PADl4다태성재결직장암발병과정중적작용궤제.방법채용병례-대조연구,용TaqMan-PCR법대515례결직장암환자화549례건강대조자적3개표첨단핵감산다태성(TagSNP)위점,즉rs1886302、rs247713l화rs1635561진행기인분형,사용SHEsis화SPSS11.5연건대결과진행x2검험화Logistic회귀분석.결과병례조여대조조관련성분석발현,위우PADI4기인내함자1구적rsl886302위점등위기인차이유통계학의의(P<0.05),기인형현성유전모식차이야유통계학의의(P<0.05),등위기인A화기인형AA적OR치분별위0.796화0.731,휴대등위기인A적개체회감소환결직장암적개솔.이위우PADl4내함자1화내함자15구적rs2477131화rs1635561위점등위기인화기인형차이균무현저의의(P>0.05).결론PADl4기인시결직장암유전역감기인,rsl886302시결직장암적역감성위점.
Objective We aim to investigate the association between peptidylarginine deiminase type 4 (PADl4) and colorectal cancer by genotyping threetag single nucleotide polymorphisms (tagSNP) genetic maker, to explore the role of polymorphism of PAD14 gene in development of colorectal cancer. Methods A case-control study was conducted using TaqMan-PCR methods to analyze the genotypes of three TagSNPs such as rs1886302, rs2477131 and rs1635561 for 515 patients with colorectal cancer and 549 health controls. Results There is significantly different in allelic frequencies and genotype frequencies of rs1886302 between cases and controls (P=0.039 and 0.040,respectively). The OR value of A allele and AA genotype is 0.796 and 0.731,respectively,and A allele may reduce the probability of incidence of colorectal cancer. No association Was found among SNPs of rs2477131 and rs1635561 within intronl and intron 15 in terms of coloretal cancer. Conclusion Rs1886302 on PADI4 might be a susceptible SNP to coloretal cancer.
