甲状腺结节%肿瘤坏死因子相关凋亡诱导配体%基因频率%多态性,限制性片段长度
甲狀腺結節%腫瘤壞死因子相關凋亡誘導配體%基因頻率%多態性,限製性片段長度
갑상선결절%종류배사인자상관조망유도배체%기인빈솔%다태성,한제성편단장도
Thyroid nodule%Tumor necrosis factor related apoptosis inducing ligand%Gene frequency%Polymorphism,restriction fragment length
目的 探讨肿瘤坏死因子相关凋亡诱导配体基因(TRAIL)1525位点多态性与结节性甲状腺疾病的关系.方法 125例甲状腺疾病患者来自于内蒙古包头医学院第一附属医院内分泌科,其中结节性甲状腺肿(结甲)患者67例,甲状腺腺瘤患者58例.患者中男54例、女71例,平均年龄为(41.05±14.42)岁.结甲患者接毒性或非毒性进行分组,甲状腺腺瘤患者按高功能或非高功能分组.正常对照人群100例,来自本院同期体检者,其中男47例,女53例,平均年龄为(42.35±16.52)岁.根据知情同意的原则,采集两组人群的静脉血,采用聚合酶链反应-限制性内切酶片段长度多态性(PCR-RLFP)方法进行TRAIL基因多态性测定,计算TRAIL基因的基因型(纯合子GG、杂合子GA、突变纯合子AA)和等位基因频率(G、A),比较相对风险度(比值比,OR).结果 结甲组TRAIL基因1525位点基因型(GG:40.3％,AG:44.8％,AA:14.9％)、等位基因频率(G:62.7％,A:37.3％)与对照组(GG:17.0％,AG:65.0％,AA:18.0％;G:49.5％,A:50.5％)比较,差异有统计学意义(x2值分别为11.376、5.633,P＜0.01或＜0.05)；腺瘤组1525位点基因型(GG:44.8％,AG:38.0％,AA:17.2％)、等位基因频率(G:63.8％,A:36.2％)与对照组比较,差异有统计学意义(x2值分别为15.342、6.054,P＜0.01或＜ 0.05).结甲组TRAIL基因1525位点等位基因频率风险度与对照组的比较,OR=1.714(P＜ 0.05),95％可信区间(CI):1.097 - 2.679;腺瘤组TRAIL基因1525位点等位基因频率风险度与对照组的比较,OR=1.797(P＜0.05),95％CI:1.124～2.874.毒性与非毒性结节组、高功能与非高功能腺瘤组TRAIL基因1525位点基因型、等位基因频率比较,差异无统计学意义(x2值分别为3.714,2.792;1.103,2.020；P均＞0.05).结论 TRAIL 基因1525位点多态性与结节性甲状腺疾病发生关系密切.
目的 探討腫瘤壞死因子相關凋亡誘導配體基因(TRAIL)1525位點多態性與結節性甲狀腺疾病的關繫.方法 125例甲狀腺疾病患者來自于內矇古包頭醫學院第一附屬醫院內分泌科,其中結節性甲狀腺腫(結甲)患者67例,甲狀腺腺瘤患者58例.患者中男54例、女71例,平均年齡為(41.05±14.42)歲.結甲患者接毒性或非毒性進行分組,甲狀腺腺瘤患者按高功能或非高功能分組.正常對照人群100例,來自本院同期體檢者,其中男47例,女53例,平均年齡為(42.35±16.52)歲.根據知情同意的原則,採集兩組人群的靜脈血,採用聚閤酶鏈反應-限製性內切酶片段長度多態性(PCR-RLFP)方法進行TRAIL基因多態性測定,計算TRAIL基因的基因型(純閤子GG、雜閤子GA、突變純閤子AA)和等位基因頻率(G、A),比較相對風險度(比值比,OR).結果 結甲組TRAIL基因1525位點基因型(GG:40.3％,AG:44.8％,AA:14.9％)、等位基因頻率(G:62.7％,A:37.3％)與對照組(GG:17.0％,AG:65.0％,AA:18.0％;G:49.5％,A:50.5％)比較,差異有統計學意義(x2值分彆為11.376、5.633,P＜0.01或＜0.05)；腺瘤組1525位點基因型(GG:44.8％,AG:38.0％,AA:17.2％)、等位基因頻率(G:63.8％,A:36.2％)與對照組比較,差異有統計學意義(x2值分彆為15.342、6.054,P＜0.01或＜ 0.05).結甲組TRAIL基因1525位點等位基因頻率風險度與對照組的比較,OR=1.714(P＜ 0.05),95％可信區間(CI):1.097 - 2.679;腺瘤組TRAIL基因1525位點等位基因頻率風險度與對照組的比較,OR=1.797(P＜0.05),95％CI:1.124～2.874.毒性與非毒性結節組、高功能與非高功能腺瘤組TRAIL基因1525位點基因型、等位基因頻率比較,差異無統計學意義(x2值分彆為3.714,2.792;1.103,2.020；P均＞0.05).結論 TRAIL 基因1525位點多態性與結節性甲狀腺疾病髮生關繫密切.
목적 탐토종류배사인자상관조망유도배체기인(TRAIL)1525위점다태성여결절성갑상선질병적관계.방법 125례갑상선질병환자래자우내몽고포두의학원제일부속의원내분비과,기중결절성갑상선종(결갑)환자67례,갑상선선류환자58례.환자중남54례、녀71례,평균년령위(41.05±14.42)세.결갑환자접독성혹비독성진행분조,갑상선선류환자안고공능혹비고공능분조.정상대조인군100례,래자본원동기체검자,기중남47례,녀53례,평균년령위(42.35±16.52)세.근거지정동의적원칙,채집량조인군적정맥혈,채용취합매련반응-한제성내절매편단장도다태성(PCR-RLFP)방법진행TRAIL기인다태성측정,계산TRAIL기인적기인형(순합자GG、잡합자GA、돌변순합자AA)화등위기인빈솔(G、A),비교상대풍험도(비치비,OR).결과 결갑조TRAIL기인1525위점기인형(GG:40.3％,AG:44.8％,AA:14.9％)、등위기인빈솔(G:62.7％,A:37.3％)여대조조(GG:17.0％,AG:65.0％,AA:18.0％;G:49.5％,A:50.5％)비교,차이유통계학의의(x2치분별위11.376、5.633,P＜0.01혹＜0.05)；선류조1525위점기인형(GG:44.8％,AG:38.0％,AA:17.2％)、등위기인빈솔(G:63.8％,A:36.2％)여대조조비교,차이유통계학의의(x2치분별위15.342、6.054,P＜0.01혹＜ 0.05).결갑조TRAIL기인1525위점등위기인빈솔풍험도여대조조적비교,OR=1.714(P＜ 0.05),95％가신구간(CI):1.097 - 2.679;선류조TRAIL기인1525위점등위기인빈솔풍험도여대조조적비교,OR=1.797(P＜0.05),95％CI:1.124～2.874.독성여비독성결절조、고공능여비고공능선류조TRAIL기인1525위점기인형、등위기인빈솔비교,차이무통계학의의(x2치분별위3.714,2.792;1.103,2.020；P균＞0.05).결론 TRAIL 기인1525위점다태성여결절성갑상선질병발생관계밀절.
Objective To study tumor necrosis factor-related apoptosis-inducing ligand(TRAIL) 1525 locus polymorphism in patients with nodular thyroid disease and investigate the relation between individual gene polymorphism and thyroid diseases.Methods A total of 125 patients were diagnosed with nodular thyroid disease at the Department of Endocrinology,the First Affiliated Hospital of Inner Mongolia Medical College.Among these patients,67 cases were nodular thyroid goiter and 58 cases were nodular thyroid adenoma,54 males,71 females,and average age was 41.05 ± 14.42. Patients with nodular thyroid goiter were grouped into toxic and non-toxic and thyroid adenoma were grouped into high-functioning or non-high-functioning.A total of 100 healthy subjects.47 males,53 females,average age 42.35 ± 16.52 were as control group.According to the principle of informed consent,venous blood was collected,polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method for TRAIL gene 1525 locus polymorphism determination was performed to calculate genotype of the TRAIL gene (homozygous GG,heterozygous GA,mutationhomozygous AA) and the gene frequency (G,A),and relative degree of risk(odd ratio,OR) was compared.Results Nodular goiter group TRAIL gene 1525 locus genotype frequencies(GG:40.3％,AG:44.8％,AA:14.9％),allele frequencies(G:62.7％,A:37.3％) were compared with that of the control group(GG:17.0％,AG:65.0％,AA:18.0％; G:49.5％,A:50.5％),the differences were statistically significant(x2 =11.376,5.633,P ＜ 0.01 or ＜ 0.05 ).Adenomas group 1525 locus genotype frequencies of the TRAIL gene(GG:44.8％,AG:38.0％,AA:17.2％),allele frequencies(G:63.8％,A:36.2％) were compared with that of the control group,the differences were statistically significant(x2 =15.342,6.054,P ＜ 0.01 or ＜ 0.05).Allele frequencies of thyroid goiter group and denoma group were compared with that of the control group,OR values were 1.714 and 1.797(all P ＜ 0.05) and 95％ confidence intervals were 1.097 - 2.679 and 1.124 - 2.874.The difference of 1525 locus genotype or allele frequency distribution in toxic and non-toxic nodular group,high functioning and non-high-functioning adenomas group were not statistically significant (x2 =3.714,2.792; 1.103,2.020; all P ＞ 0.05).Conclusion TRAIL gene 1525 locus polymorphism is significantly associated with nodular thyroid disease.
