CAJ | 학술논문

目的了解老年人胃壁细胞超微结构、氢-钾三磷酸腺苷酶(H+ -K+ -ATP酶)亚基mRNA及蛋白的增龄性变化. 方法选择无胃十二指肠疾病者50例为研究对象,其中中青年组(20～59岁)19例和老年组(≥60岁)31例,老年组中60～69岁11例,70～79岁10例,80岁以上10例.电镜下观察胃壁细胞的超微结构,采用荧光定量PCR测定胃壁细胞H+-K+ -ATP酶α亚基mRNA的表达,采用免疫印记法检测胃壁细胞H+-K+ -ATP酶β亚基蛋白的表达,并分别比较其增龄性变化. 结果电镜下观察各年龄组胃壁细胞及与泌酸功能相关的细胞器形态分布无明显差异,定量测定线粒体面积分数中青年组为(48.4±7.5)％,与老年组(50.6±7.6)％比较,差异无统计学意义(t=-0.775,P=0.444)；管泡系统面积分数中青年组为(13.8±4.1)％,老年组为(12.2±4.7)％,差异无统计学意义(t=0.984,P=0.332).老年组H+ -K+ -ATP酶α亚基mRNA的表达(t=-3.682,P=0.001)及H+-K+-ATP酶β亚基蛋白(t=-3.389,P=0.004)均显著高于中青年组,但中青年组与老年各年龄组间趋势方差分析显示,H+ -K+ -ATP酶mRNA的表达(F=1.522,P=0.24)及H+-K+ -ATP酶蛋白的表达(F=2.32,P=0.114)差异无统计学意义. 结论随增龄老年人胃壁细胞内与泌酸功能直接相关的细胞器无退化表现,H+-K+-ATP酶表达有增加趋势,提示健康老年人存在维持良好泌酸功能的超微结构和分子生物学物质基础.
목적 료해노년인위벽세포초미결구、경-갑삼린산선감매(H+ -K+ -ATP매)아기mRNA급단백적증령성변화. 방법 선택무위십이지장질병자50례위연구대상,기중중청년조(20～59세)19례화노년조(≥60세)31례,노년조중60～69세11례,70～79세10례,80세이상10례.전경하관찰위벽세포적초미결구,채용형광정량PCR측정위벽세포H+-K+ -ATP매α아기mRNA적표체,채용면역인기법검측위벽세포H+-K+ -ATP매β아기단백적표체,병분별비교기증령성변화. 결과 전경하관찰각년령조위벽세포급여비산공능상관적세포기형태분포무명현차이,정량측정선립체면적분수중청년조위(48.4±7.5)％,여노년조(50.6±7.6)％비교,차이무통계학의의(t=-0.775,P=0.444)；관포계통면적분수중청년조위(13.8±4.1)％,노년조위(12.2±4.7)％,차이무통계학의의(t=0.984,P=0.332).노년조H+ -K+ -ATP매α아기mRNA적표체(t=-3.682,P=0.001)급H+-K+-ATP매β아기단백(t=-3.389,P=0.004)균현저고우중청년조,단중청년조여노년각년령조간추세방차분석현시,H+ -K+ -ATP매mRNA적표체(F=1.522,P=0.24)급H+-K+ -ATP매단백적표체(F=2.32,P=0.114)차이무통계학의의. 결론 수증령노년인위벽세포내여비산공능직접상관적세포기무퇴화표현,H+-K+-ATP매표체유증가추세,제시건강노년인존재유지량호비산공능적초미결구화분자생물학물질기출.
Objective To investigate the age-associated changes of ultrastructure,mRNA and protein expressions of H+-K+-ATPase in elderly gastric parietal cell. Methods Fifty patients with relative normal stomach without gastroduodenal diseases were enrolled,including younger group (aged 20-59 years,n=19) and elderly group (aged≥60 years,n=31).Furthermore,the elderly group was divided into 3 subgroups:60-69 years old (n =11 ),70-79 years old (n=10 ),above 80 years old (n =10).The ultrastructure of gastric parietal cell was observed under electron microscope.The expression of H+-K+-ATPase α subunit mRNA and H+-K+-ATPase β subunit protein were assessed by quantitative real-time PCR and Western-blot,respectively.The ageing-associated changes of all these data were respectively compared. Results No significant difference was showed in the morphology of gastric parietal cell and acid-secretion-associated organelles among all the groups.The average ratio Am to Ac (Am means the area of mitochondria,Ac means the area of cytoplasm) of gastric parietal cell and the average At to Ac ratio (At means the area of secretory canaliculi and tubulovesicular system )between younger group and elderly group had no significant difference[(48.4±7.5) ％ vs.(50.6±7.6) ％,t=-0.775,P=0.444; (13.8±4.1) ％ vs.(12.2±4.7) ％,t=0.984,P=0.332].Meanwhile,there were no distinctions in the expression of H+-K+ -ATPase α subunit mRNA and H+-K+-ATPase protein among all elderly subgroups(F=1.522,2.32,P=0.24,0.114).However,the mRNA expression of H+-K+-ATPase a subunit was higher in the elderly group than in the younger group(t=-3.682,P=0.001).Furthermore,the expression of H+ -K+ -ATPase protein in the elderly group was increased as compared with younger group(t=-3.389,P=0.004). Conclusions Acidsecretion-associated organelles of human gastric parietal cell have no degeneration and the expression of H + -K+-ATPase is in trend of increase with aging,indicating that healthy elderly people have the basis of ultrastructure and molecular biology to maintain well function of acid secretion.