中华临床感染病杂志
中華臨床感染病雜誌
중화림상감염병잡지
CHINESE JOURNAL OF CLINICAL INFECTIOUS DISEASES
2010年
4期
217-221
,共5页
徐瑞龙%应华永%诸葛盼%卜黎红%尤慧华
徐瑞龍%應華永%諸葛盼%蔔黎紅%尤慧華
서서룡%응화영%제갈반%복려홍%우혜화
生物膜%慢性鼻-鼻窦炎%显微镜检查,电子%扫描%细菌培养%抗药性
生物膜%慢性鼻-鼻竇炎%顯微鏡檢查,電子%掃描%細菌培養%抗藥性
생물막%만성비-비두염%현미경검사,전자%소묘%세균배양%항약성
Biofilm%Chronic rhinosinusitis%Microscopy,electron%Scanning%Bacterial culture%Drug resistance
目的 探讨细菌生物膜在慢性鼻-鼻窦炎(chronic rhinosinusitis,CRS)患者鼻黏膜中的表达与细菌培养的相关性.方法 获取90例CRS患者的手术黏膜样本,并以5例鼻中隔偏曲患者及10名健康志愿者的钩突黏膜样本作为对照.所有样本均行细菌培养及扫描电子显微镜检查,细菌生物膜表达与细菌培养的相关性分析采用Pearson检验.结果 90例实验组患者中,64例观察到细菌生物膜,阳性率为71.1%,其中60例(66.7%)患者细菌培养阳性.实验共检出61株细菌,其中检出率最高的为表皮葡萄球菌,占41.0%(25/61).对照组及其余26例实验组患者均未观察到细菌生物膜,也未培养出需氧或厌氧菌.细菌培养与细菌生物膜间的Pearson相关系数为0.901(P=0.000).结论 CRS患者鼻黏膜中细菌生物膜表达与细菌培养阳性结果高度相关.
目的 探討細菌生物膜在慢性鼻-鼻竇炎(chronic rhinosinusitis,CRS)患者鼻黏膜中的錶達與細菌培養的相關性.方法 穫取90例CRS患者的手術黏膜樣本,併以5例鼻中隔偏麯患者及10名健康誌願者的鉤突黏膜樣本作為對照.所有樣本均行細菌培養及掃描電子顯微鏡檢查,細菌生物膜錶達與細菌培養的相關性分析採用Pearson檢驗.結果 90例實驗組患者中,64例觀察到細菌生物膜,暘性率為71.1%,其中60例(66.7%)患者細菌培養暘性.實驗共檢齣61株細菌,其中檢齣率最高的為錶皮葡萄毬菌,佔41.0%(25/61).對照組及其餘26例實驗組患者均未觀察到細菌生物膜,也未培養齣需氧或厭氧菌.細菌培養與細菌生物膜間的Pearson相關繫數為0.901(P=0.000).結論 CRS患者鼻黏膜中細菌生物膜錶達與細菌培養暘性結果高度相關.
목적 탐토세균생물막재만성비-비두염(chronic rhinosinusitis,CRS)환자비점막중적표체여세균배양적상관성.방법 획취90례CRS환자적수술점막양본,병이5례비중격편곡환자급10명건강지원자적구돌점막양본작위대조.소유양본균행세균배양급소묘전자현미경검사,세균생물막표체여세균배양적상관성분석채용Pearson검험.결과 90례실험조환자중,64례관찰도세균생물막,양성솔위71.1%,기중60례(66.7%)환자세균배양양성.실험공검출61주세균,기중검출솔최고적위표피포도구균,점41.0%(25/61).대조조급기여26례실험조환자균미관찰도세균생물막,야미배양출수양혹염양균.세균배양여세균생물막간적Pearson상관계수위0.901(P=0.000).결론 CRS환자비점막중세균생물막표체여세균배양양성결과고도상관.
Objective To investigate the relationship between bacteria biofilm and bacterial culture in patients with chronic rhinosinusitis (CRS). Methods Ninety patients with CRS were enrolled in the study. Five patients with deviation of nasal septum and 10 healthy subjects served as controls. Mucosa of uncinate process or near the ostium of the maxillary sinus was obtained during endoscopic sinus surgery. All specimens were processed for bacterial culture and scanned by electron microscopy. Pearson test was performed to analyze the relationship between the presence of bacterial biofilm and the results of bacteria culture. Results The scanning electron microscopy showed bacterial biofilms in 64 (71.1%) out of 90patients with CRS, while the positive rate of bacteria culture in the study group was 66.7% (60/90). No bacterial biofilm and bacterium was detected in the control group and 26 culture-negative individuals in study group. Pearson correlation analysis showed a statistically association between bacterial biofilm and bacterial culture in CRS ( r = 0. 901, P = 0. 000). Conclusion Positive results of bacteria culture are highly correlated with the presence of bacterial biofilm in CRS patients.