中华口腔医学杂志
中華口腔醫學雜誌
중화구강의학잡지
Chinese Journal of Stomatology
2009年
6期
355-359
,共5页
刘颍凤%王小竞%王庆昱%张晓%王军
劉潁鳳%王小競%王慶昱%張曉%王軍
류영봉%왕소경%왕경욱%장효%왕군
尼古丁%大鼠,Sprague-Dawley%牙周炎%体层摄影术,X线计算机
尼古丁%大鼠,Sprague-Dawley%牙週炎%體層攝影術,X線計算機
니고정%대서,Sprague-Dawley%아주염%체층섭영술,X선계산궤
Nicotine%Rats,Sprague-Dawley%Periodontitis%Tomography,X-ray computed
目的 建立牙周炎大鼠的牙槽骨三维模型,采用显微CT观察尼古丁对大鼠牙槽骨的影响.方法 36只SD大鼠,丝线结扎上颌右侧(实验侧)第二磨牙颈部,左侧不予结扎,作为自身对照(对照侧),使用完全随机分组方法分为对照组(A)及尼古丁注射低剂量(B)和高剂量(C)组,每组12只.分别给予生理盐水和尼古丁0.83、1.67 mg·kg-1·d-1腹腔注射.每组分别于给药后第14、28天各处死6只,取双侧上颌磨牙区牙体牙周复合组织,行显微CT扫描、重建、测最和分析.结果 随尼古丁给药剂量增加,双侧牙槽骨骨密度、骨体积分数、骨小梁厚度逐渐降低,牙槽骨高度丧失与骨小梁间隙逐渐升高.28 d时C组牙槽骨高度丧失[对照侧和实验侧分别为(0.61±0.14)、(1.39±0.09)mm]显著高于B组[对照侧和实验侧分别为(0.39±0.10)、(1.31±0.06)mm]和A组[对照侧和实验侧分别为(0.30±0.06)、(0.94±0.07)mm];C组牙槽骨骨密度[对照侧和实验侧分别为(617.86±34.27)、(572.46±31.62)mg/cm3]显著低于B组[对照侧和实验侧分别为(660.04±36.73)、(604.97±32.59)mg/cm3]和A组[对照侧和实验侧分别为(709.15±34.95)、(657.04±30.06)mg/cm3].结论 尼古丁可加重丝线结扎造成的大鼠牙槽骨骨量丧失和骨质微观结构的变化,导致牙槽骨骨质疏松.
目的 建立牙週炎大鼠的牙槽骨三維模型,採用顯微CT觀察尼古丁對大鼠牙槽骨的影響.方法 36隻SD大鼠,絲線結扎上頜右側(實驗側)第二磨牙頸部,左側不予結扎,作為自身對照(對照側),使用完全隨機分組方法分為對照組(A)及尼古丁註射低劑量(B)和高劑量(C)組,每組12隻.分彆給予生理鹽水和尼古丁0.83、1.67 mg·kg-1·d-1腹腔註射.每組分彆于給藥後第14、28天各處死6隻,取雙側上頜磨牙區牙體牙週複閤組織,行顯微CT掃描、重建、測最和分析.結果 隨尼古丁給藥劑量增加,雙側牙槽骨骨密度、骨體積分數、骨小樑厚度逐漸降低,牙槽骨高度喪失與骨小樑間隙逐漸升高.28 d時C組牙槽骨高度喪失[對照側和實驗側分彆為(0.61±0.14)、(1.39±0.09)mm]顯著高于B組[對照側和實驗側分彆為(0.39±0.10)、(1.31±0.06)mm]和A組[對照側和實驗側分彆為(0.30±0.06)、(0.94±0.07)mm];C組牙槽骨骨密度[對照側和實驗側分彆為(617.86±34.27)、(572.46±31.62)mg/cm3]顯著低于B組[對照側和實驗側分彆為(660.04±36.73)、(604.97±32.59)mg/cm3]和A組[對照側和實驗側分彆為(709.15±34.95)、(657.04±30.06)mg/cm3].結論 尼古丁可加重絲線結扎造成的大鼠牙槽骨骨量喪失和骨質微觀結構的變化,導緻牙槽骨骨質疏鬆.
목적 건립아주염대서적아조골삼유모형,채용현미CT관찰니고정대대서아조골적영향.방법 36지SD대서,사선결찰상합우측(실험측)제이마아경부,좌측불여결찰,작위자신대조(대조측),사용완전수궤분조방법분위대조조(A)급니고정주사저제량(B)화고제량(C)조,매조12지.분별급여생리염수화니고정0.83、1.67 mg·kg-1·d-1복강주사.매조분별우급약후제14、28천각처사6지,취쌍측상합마아구아체아주복합조직,행현미CT소묘、중건、측최화분석.결과 수니고정급약제량증가,쌍측아조골골밀도、골체적분수、골소량후도축점강저,아조골고도상실여골소량간극축점승고.28 d시C조아조골고도상실[대조측화실험측분별위(0.61±0.14)、(1.39±0.09)mm]현저고우B조[대조측화실험측분별위(0.39±0.10)、(1.31±0.06)mm]화A조[대조측화실험측분별위(0.30±0.06)、(0.94±0.07)mm];C조아조골골밀도[대조측화실험측분별위(617.86±34.27)、(572.46±31.62)mg/cm3]현저저우B조[대조측화실험측분별위(660.04±36.73)、(604.97±32.59)mg/cm3]화A조[대조측화실험측분별위(709.15±34.95)、(657.04±30.06)mg/cm3].결론 니고정가가중사선결찰조성적대서아조골골량상실화골질미관결구적변화,도치아조골골질소송.
Objective To establish the three-dimensional images of rat's alveolar bone and to evaluate the effects of nicotine on alveolar bone loss during the process of ligature-induced periodontitis with micro-computed tomography(micro-CT).Methods Thirty-six adult male SD rats received silk ligatures around the cervlxes of the Tight second maxillary molars.Then the animals were randomly assigned to three groups and received daily intraperitoneal injections as follows:group A(control),saline solution;group B,were randomly selected and sacrificed at day 14 and 28.Micro-CT examinations were used to evaluate the periodontal breakdown.Results With the nicotine dose increased,bone mineral density(BMD),bone volume fraction(BVF),and trabecular thickness(TT)gradually reduced,while the trabecular spacing(TS)and alveolar bone loss(ABL)increased.At day 28,the ABL of group C(left,right)was(0.61±0.14)mm and(1.39±0.09)mm,and significantly higher than that of group B[(0.39±0.10)mm and(1.31±0.06)mm]and group A[(0.30±0.06)mm and(0.94±0.07)mm].The BMD of group C,group B and group A at day 28 was[(617.86±34.27),(572.46±31.62)mg/cm3],[(660.04±36.73),(604.97±32.59)mg/cm3]and[(709.15±34.95),(657.04±30.06)mg/cm3]respectively.Conclusions Daily administration of nicotine results in significant bone loss and microstructure deteriorations in the trabeculae of alveolar bone.
