CAJ | 학술논문

目的研究携带IL-24基因的溶瘤腺病毒对肝癌细胞抑制生长和转移的作用.方法构建Ad.HS4.AFP.E1A/IL-24,病毒的复制由人甲胎蛋白启动子控制,携带IL-24基因.检测病毒在不同细胞系中的选择性复制,以及对高转移潜能人肝癌细胞系MHCC97-H的生长抑制、诱导凋亡和抑制转移能力(侵袭、运动、黏附)的作用.结果 Ad.HS4.AFP.E1A/IL-24在肝癌细胞SMMC-7721、Hep3B和MHCC97-H中选择性表达,而不影响正常肝细胞L02(P＜0.05).Ad.HS4.AFP.E1A/IL-24可明显抑制MHCC97-H细胞的增殖,诱导其凋亡,并抑制其体外运动、侵袭和黏附能力(P＜0.01).RT-PCR和明胶酶谱显示其抑制肝癌作用与抑制MMP-2的表达有关.结论携带IL-24基因的溶瘤腺病毒能够选择性抑制肝癌细胞的增殖并抑制其转移.
목적 연구휴대IL-24기인적용류선병독대간암세포억제생장화전이적작용.방법 구건Ad.HS4.AFP.E1A/IL-24,병독적복제유인갑태단백계동자공제,휴대IL-24기인.검측병독재불동세포계중적선택성복제,이급대고전이잠능인간암세포계MHCC97-H적생장억제、유도조망화억제전이능력(침습、운동、점부)적작용.결과 Ad.HS4.AFP.E1A/IL-24재간암세포SMMC-7721、Hep3B화MHCC97-H중선택성표체,이불영향정상간세포L02(P＜0.05).Ad.HS4.AFP.E1A/IL-24가명현억제MHCC97-H세포적증식,유도기조망,병억제기체외운동、침습화점부능력(P＜0.01).RT-PCR화명효매보현시기억제간암작용여억제MMP-2적표체유관.결론 휴대IL-24기인적용류선병독능구선택성억제간암세포적증식병억제기전이.
Objective To investigate the selective oncolytic role and antitumor action of a novel recombinant adenovirus containing E1A and IL-24 on hepatocellular carcinoma cell(HCC). Methods The recombinant adenovirus expressing IL-24 (Ad. HS4. AFP. E1A/IL-24) was constructed by using modified human alpha-fetoprotein (HS4-AFP) promoter to drive adenovirus E1A gene and II-24 gene.Cell Counting Kit-8 were performed to test the selective cytotoxicity of the virus in hepatocellular carcinoma cell lines SMMC-7721, Hep3B, MHCC97-H and hepatocyte cell line L02 . The mRNA and protein expression of IL-24 gene were detected by RT-PCR and western blot. Cell growth curves and Annexin V/PI assay were used to study cell proliferation and apoptosis of MHCC97-H. The anti-metastatic effects of the recombinant adenovirus were evaluated in cell adhesion, migration, and cell motion. Matrix metalloproteinase-2 (MMP-2) expression was examined by RT-PCR and zymography.Results Selective replications of Ad. HS4. AFP. E1A/IL-24 adenovirus were observed in over expression AFP cell line MHCC97-H, a highly metastatic potential HCC cell line but not in hepatocyte cell line L02. The mRNA and protein of IL-24 were also over expressed in MHCC97-H. This recombinant adenovirus also showed the significant oncolytic action on MHCC97-H but not on L02 (P＜0. 05). Besides, the recombinant adenovirus significantly inhibited MHCC97-H metastatic potential such as cell adhesion, migration and invasion as well(P＜0.01). Conclusion The selective oncolytic adenovirus expressing E1A and II-24 has a selective antitumor effect and play an inhibitory role in metastasis of HCC.