CAJ | 학술논문

目的观察神经干细胞(NSC)、许旺细胞(SCs)和组织工程材料乙交酯-丙交酯共聚物(PLGA)大鼠髓内共移植后的病理形态学改变.方法 36只Wistar大鼠,随机分为PLGA移植组、NSC/PLGA组和NSC+SCs/PLGA组.体外培养、鉴定胚胎脊髓源NSC和SCs,制备和构建PLGA支架细胞复合体并移植到大鼠脊髓Tq半横断损伤部位,应用HE染色、电镜和免疫组织化学染色方法在形态结构上观察材料的组织相容性、轴突髓鞘再生及NSC在脊髓内的存活、迁移和分化情况.结果 HE染色观察损伤12周时移植材料内可见细胞生长及新生的毛细血管;扫描电镜观察随着时间的延长,PLGA逐渐降解;材料正中横断面透射电镜观察可见新牛的无髓及有髓神经纤维;脊髓标本免疫组织化学染色可见移植的NSC可以在宿主脊髓内存活、迁移并分化成类神经元样细胞和少枝胶质细胞,未分化成星形胶质细胞.结论 NSC、SCs和PLGA共移植可以在形态学上促进大鼠脊髓半横断损伤的修复.
목적 관찰신경간세포(NSC)、허왕세포(SCs)화조직공정재료을교지-병교지공취물(PLGA)대서수내공이식후적병리형태학개변.방법 36지Wistar대서,수궤분위PLGA이식조、NSC/PLGA조화NSC+SCs/PLGA조.체외배양、감정배태척수원NSC화SCs,제비화구건PLGA지가세포복합체병이식도대서척수Tq반횡단손상부위,응용HE염색、전경화면역조직화학염색방법재형태결구상관찰재료적조직상용성、축돌수초재생급NSC재척수내적존활、천이화분화정황.결과 HE염색관찰손상12주시이식재료내가견세포생장급신생적모세혈관;소묘전경관찰수착시간적연장,PLGA축점강해;재료정중횡단면투사전경관찰가견신우적무수급유수신경섬유;척수표본면역조직화학염색가견이식적NSC가이재숙주척수내존활、천이병분화성류신경원양세포화소지효질세포,미분화성성형효질세포.결론 NSC、SCs화PLGA공이식가이재형태학상촉진대서척수반횡단손상적수복.
Objective To observe the pathological change of the co-transplantation of neural stem cells(NSC),Schwann cells(SOs)and PLGA to the spinal cord injury in rats.Method Thirty-six Wistar rats were divided into three groups randomly:PLGA,NSC/PLGA and NSC+SCs/PLGA groups.The complex of PLGA-cells was constructed and implanted into the injury site of T9 SCI models developed from Wistar rats.All the SCI tissues were observed with electromicroscope,HE and immunohistochemistry to identify the bioeompatibility of PLGA,regeneration of neural fibers and the survival,migration and differentiation of the transplanted cells at 4.8 and 12 weeks after injury.Results Different cells and regenerated blood capillary were detected in the scaffolds through HE stain.The PLGA gradually degenerated under scanning electromicroscope.The regenerated axons were observed in the scaffold-cells complex with electromicroscope.Immunohistochemistry staining showed the transplanted NSC could survive and migrate at 12 weeks and differentiated into neurons and oligodendrocytes.Conclusions Co-transplantation of NSC +SCs/PLGA can morphologically promote the reparation of the injured spinal cord.