中华神经医学杂志
中華神經醫學雜誌
중화신경의학잡지
CHINESE JOURNAL OF NEUROMEDICINE
2012年
7期
658-662
,共5页
脆性X综合征%树突棘%Drebrins%lcam-5
脆性X綜閤徵%樹突棘%Drebrins%lcam-5
취성X종합정%수돌극%Drebrins%lcam-5
Fragile X Syndrome%Dendritic spine%Drebrins%lcam-5
目的 观察脆性X综合征(FXS)模型小鼠不同发育时期大脑皮层中Drebrin A、Drebrin E及Icam-5 mRNA水平变化情况及意义.方法 应用荧光实时定量PCR(RT-PCR)法检测FmrJ基因敲除KO小鼠及野生健康对照小鼠H出生后第7天、第14天、第21天和第28天大脑皮层Drebrin A、Drebrin E及Icam-5 mRNA的表达(4≤n≤10).结果 KO组小鼠出生后第14天Drebrin A mRNA水平较健康对照组小鼠明显降低,而同时间Drebrin E mRNA水平较健康对照组小鼠明显增高,差异均有统计学意义(P＜0.05);KO组小鼠Icam-5 mRNA水平在出生后第14和21天均明显高于健康对照组,差异均有统计学意义(P＜0.05).结论 Drebrin A和Drebrin E在大脑皮层发育期的表达交替延迟及Icam-5的一过性过度表达是FXS智力低下的原因之一.
目的 觀察脆性X綜閤徵(FXS)模型小鼠不同髮育時期大腦皮層中Drebrin A、Drebrin E及Icam-5 mRNA水平變化情況及意義.方法 應用熒光實時定量PCR(RT-PCR)法檢測FmrJ基因敲除KO小鼠及野生健康對照小鼠H齣生後第7天、第14天、第21天和第28天大腦皮層Drebrin A、Drebrin E及Icam-5 mRNA的錶達(4≤n≤10).結果 KO組小鼠齣生後第14天Drebrin A mRNA水平較健康對照組小鼠明顯降低,而同時間Drebrin E mRNA水平較健康對照組小鼠明顯增高,差異均有統計學意義(P＜0.05);KO組小鼠Icam-5 mRNA水平在齣生後第14和21天均明顯高于健康對照組,差異均有統計學意義(P＜0.05).結論 Drebrin A和Drebrin E在大腦皮層髮育期的錶達交替延遲及Icam-5的一過性過度錶達是FXS智力低下的原因之一.
목적 관찰취성X종합정(FXS)모형소서불동발육시기대뇌피층중Drebrin A、Drebrin E급Icam-5 mRNA수평변화정황급의의.방법 응용형광실시정량PCR(RT-PCR)법검측FmrJ기인고제KO소서급야생건강대조소서H출생후제7천、제14천、제21천화제28천대뇌피층Drebrin A、Drebrin E급Icam-5 mRNA적표체(4≤n≤10).결과 KO조소서출생후제14천Drebrin A mRNA수평교건강대조조소서명현강저,이동시간Drebrin E mRNA수평교건강대조조소서명현증고,차이균유통계학의의(P＜0.05);KO조소서Icam-5 mRNA수평재출생후제14화21천균명현고우건강대조조,차이균유통계학의의(P＜0.05).결론 Drebrin A화Drebrin E재대뇌피층발육기적표체교체연지급Icam-5적일과성과도표체시FXS지력저하적원인지일.
[Objective]To investigate and compare the changes of Drebrin A,Drebrin E and lcam-5 mRNA levels in the cerebral cortex of Frr-1 gene knockout mouse during brain development periods.[Methods]Fmr-1 gene knockout (KO) male mice and their wild type (WT) counterparts were chosen in our experiment (4≤n≤ 10);the levels of target mRNAs were detected by real time quantitative PCR;check points were set on the 7th,14th,21th and 28rh postnatal d.[Results] The mRNA level of Drebrin A in the KO group was significantly lower than that in the WT group on the 14th postnatal d,while that of Drebrin E was significantly higher than that in the WT group (P＜0.05).The mRNA level of lcam-5 in the KO group was significantly higher than that in the WT group on the 14th and 21th postnatal d (P＜0.05).[Conclusion] The delayed shift of Drebrin A to Drebrin E and transitional over-expression of lcam-5 in developmental cerebral cortex are the reasons for mental retardation in Fragile X Syndrome.
