中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2012年
3期
438-440
,共3页
隋国德%王培戈%程广%李世宽%姜英俊%夏长所
隋國德%王培戈%程廣%李世寬%薑英俊%夏長所
수국덕%왕배과%정엄%리세관%강영준%하장소
原发性肝癌%HotStarTaq聚合酶链反应%ras%测序
原髮性肝癌%HotStarTaq聚閤酶鏈反應%ras%測序
원발성간암%HotStarTaq취합매련반응%ras%측서
Primary liver cancer%HotStarTaq polymerase chain reaction%ras%Sequencing
目的 探讨H-ras、K-ras、N-ras基因突变与原发性肝癌发生发展的关系.方法 采用HotStarTaq聚合酶链反应(PCR)检测32例原发性肝癌组织中H-ras、K-ras、N-ras基因的表达并采用PCR直接测序分析H-ras、K-ras、N-ras基因突变x2检验检测H-ras突变与临床病理的关系.结果 在32例原发性肝癌组织中均可检测到H-ras、K-ras、N-ras基因的表达,32例原发性肝癌标本PCR结果经测序后,17例标本发现H-ras基因发生突变(17/32,53.1％),14例40密码子中A突变成G,3例62密码子中G突变成A.K-ras、N-ras基因未发生突变,H-ras突变与肿瘤转移有关.结论 H-ras基因异常表达与肝癌相关,可能导致了原发性肝癌的发生.
目的 探討H-ras、K-ras、N-ras基因突變與原髮性肝癌髮生髮展的關繫.方法 採用HotStarTaq聚閤酶鏈反應(PCR)檢測32例原髮性肝癌組織中H-ras、K-ras、N-ras基因的錶達併採用PCR直接測序分析H-ras、K-ras、N-ras基因突變x2檢驗檢測H-ras突變與臨床病理的關繫.結果 在32例原髮性肝癌組織中均可檢測到H-ras、K-ras、N-ras基因的錶達,32例原髮性肝癌標本PCR結果經測序後,17例標本髮現H-ras基因髮生突變(17/32,53.1％),14例40密碼子中A突變成G,3例62密碼子中G突變成A.K-ras、N-ras基因未髮生突變,H-ras突變與腫瘤轉移有關.結論 H-ras基因異常錶達與肝癌相關,可能導緻瞭原髮性肝癌的髮生.
목적 탐토H-ras、K-ras、N-ras기인돌변여원발성간암발생발전적관계.방법 채용HotStarTaq취합매련반응(PCR)검측32례원발성간암조직중H-ras、K-ras、N-ras기인적표체병채용PCR직접측서분석H-ras、K-ras、N-ras기인돌변x2검험검측H-ras돌변여림상병리적관계.결과 재32례원발성간암조직중균가검측도H-ras、K-ras、N-ras기인적표체,32례원발성간암표본PCR결과경측서후,17례표본발현H-ras기인발생돌변(17/32,53.1％),14례40밀마자중A돌변성G,3례62밀마자중G돌변성A.K-ras、N-ras기인미발생돌변,H-ras돌변여종류전이유관.결론 H-ras기인이상표체여간암상관,가능도치료원발성간암적발생.
Objective To investigate the association between mutation of H-ras,K-ras,N-ras and carcinogenesis of primary liver cancer (PLC).Methods HotStarTaq polymerase chain reaction (PCR) was used to detect the expression of H-ras,K-ras,N-ras gene in 32 PLC patientds and the mutation of H-ras,K-ras,N-ras gene was analyzed by PCR and direct sequencing.Results The expression of H-ras,K-ras,N-ras was detected in 32 cases.Sequencing indicates that H-ras mutation occur in 17 cases,among which 14 cases was verified to be aA/G transition in codon 40 and 3 cases G/A transition in codon 62.K-ras,N-ras had no mutation.H-ras mutation was correlated to tumor metastasis.Conclusion Abnormal expression of H-ras is correlated with PLC and may lead to the the carcinogenesis of PLC.
