中华消化杂志
中華消化雜誌
중화소화잡지
Chinese Journal of Digestion
2011年
4期
239-242
,共4页
耿瑞慧%高峻%吴文彬%魏磊%肖立宁%李淑德%邹多武%李兆申
耿瑞慧%高峻%吳文彬%魏磊%肖立寧%李淑德%鄒多武%李兆申
경서혜%고준%오문빈%위뢰%초립저%리숙덕%추다무%리조신
受体,蛋白酶激活%肠易激综合征%肠黏膜%基因表达%腹泻
受體,蛋白酶激活%腸易激綜閤徵%腸黏膜%基因錶達%腹瀉
수체,단백매격활%장역격종합정%장점막%기인표체%복사
Receptors,proteinase-activated%Irritable bowel syndrome%Intestinal mucosa%Gene expression%Diarrhea
目的 研究蛋白酶激活受体(PAR)家族在腹泻型肠易激综合征(D-IBS)患者结肠黏膜中的表达变化情况.方法 入选28例D-IBS患者及18名健康对照者,经结肠镜钳取乙状结肠黏膜组织,采用Western印迹法和实时荧光定量PCR法观察蛋白酶激活受体超家族的表达情况.应用SPSS 18.0软件进行统计分析,两组间差异采用Mann-Whitney U检验.结果 PAR1在D-IBS患者和对照组结肠黏膜中的表达差异无统计学意义(P=0.300).PAR2和PAR4在D-IBS患者的结肠黏膜中均表达增加,其灰度值分别为0.99±0.67和0.37±0.14,显著高于对照组的0.63±0.38和0.25±0.11(P值分别=0.038和0.013).实时荧光定量PCR显示PAR mRNA的表达与蛋白水平相一致.结论 PAR2、PAR4的表达在D-IBS患者结肠黏膜中明显增高,可能在D-IBS疾病过程中发挥某些作用.
目的 研究蛋白酶激活受體(PAR)傢族在腹瀉型腸易激綜閤徵(D-IBS)患者結腸黏膜中的錶達變化情況.方法 入選28例D-IBS患者及18名健康對照者,經結腸鏡鉗取乙狀結腸黏膜組織,採用Western印跡法和實時熒光定量PCR法觀察蛋白酶激活受體超傢族的錶達情況.應用SPSS 18.0軟件進行統計分析,兩組間差異採用Mann-Whitney U檢驗.結果 PAR1在D-IBS患者和對照組結腸黏膜中的錶達差異無統計學意義(P=0.300).PAR2和PAR4在D-IBS患者的結腸黏膜中均錶達增加,其灰度值分彆為0.99±0.67和0.37±0.14,顯著高于對照組的0.63±0.38和0.25±0.11(P值分彆=0.038和0.013).實時熒光定量PCR顯示PAR mRNA的錶達與蛋白水平相一緻.結論 PAR2、PAR4的錶達在D-IBS患者結腸黏膜中明顯增高,可能在D-IBS疾病過程中髮揮某些作用.
목적 연구단백매격활수체(PAR)가족재복사형장역격종합정(D-IBS)환자결장점막중적표체변화정황.방법 입선28례D-IBS환자급18명건강대조자,경결장경겸취을상결장점막조직,채용Western인적법화실시형광정량PCR법관찰단백매격활수체초가족적표체정황.응용SPSS 18.0연건진행통계분석,량조간차이채용Mann-Whitney U검험.결과 PAR1재D-IBS환자화대조조결장점막중적표체차이무통계학의의(P=0.300).PAR2화PAR4재D-IBS환자적결장점막중균표체증가,기회도치분별위0.99±0.67화0.37±0.14,현저고우대조조적0.63±0.38화0.25±0.11(P치분별=0.038화0.013).실시형광정량PCR현시PAR mRNA적표체여단백수평상일치.결론 PAR2、PAR4적표체재D-IBS환자결장점막중명현증고,가능재D-IBS질병과정중발휘모사작용.
Objective To investigate the expression of protease-activated receptors (PARs) in colon mucosal tissue of patients with diarrhea-predominant irritable bowel syndrome (D-IBS). Methods Colon mucosa tissues were obtained from 28 D-IBS patients and 18 normal controls by colonoscopy.The expression of PARs was detected using Western blotting and real-time PCR. Data were analysed by SPSS 18. 0 softwave, and comparisons between groups were done using Mann-Whitney U test.Results There was no difference in expression of PAR1 between D-IBS patients and normal controls (P=0. 300). The expressions of PAR2 and PAR4 were higher in D-IBS patients than those in normal controls (0.99±0.67 vs 0.63±0.38, P=0.038 and 0.37±0. 14 vs 0.25±0. 11, P=0.013,respectively). The results of real-time PCR were in accordance with those of Western blotting.Conclusion The increased expressions of PAR2 and PAR4 in colon mucosa of D-IBS patients indicate that these two PAR receptors may be involved in the process of D-IBS.
