中华消化内镜杂志
中華消化內鏡雜誌
중화소화내경잡지
CHINESE JOURNAL OF DIGESTIVE ENDOSCOPY
2012年
6期
336-340
,共5页
关景明%李春明%张学彦%郭宇栋%马骁
關景明%李春明%張學彥%郭宇棟%馬驍
관경명%리춘명%장학언%곽우동%마효
腺瘤,结肠%癌变%细胞增殖%细胞凋亡%鸡皮样黏膜
腺瘤,結腸%癌變%細胞增殖%細胞凋亡%鷄皮樣黏膜
선류,결장%암변%세포증식%세포조망%계피양점막
Adenoma,coion%Neoplasia%Cell proliferation%Apoptosis%Chicken-skin mucosa
目的 研究伴鸡皮样黏膜( CSM)改变的成人大肠腺瘤生物学特性及其临床意义.方法 采用免疫组化SP法及酶联免疫吸附试验(ELISA)检测正常大肠黏膜、伴CSM的大肠腺瘤、不伴CSM的大肠腺瘤和大肠腺瘤癌变标本中代表细胞增殖的标志物Ki-67、COX-2和细胞凋亡相关因子survivin、caspase-3蛋白的表达情况.结果 免疫组化结果提示在大肠腺瘤癌变组织、伴CSM的大肠腺瘤、不伴CSM的大肠腺瘤及正常大肠黏膜中Ki-67、COX-2的表达呈递减趋势,而survivin和caspase-3的表达呈递增趋势.蛋白质定量分析(ELISA法)结果 提示Ki-67、COX-2、survivin、caspase-3含量在伴CSM的大肠腺瘤与腺瘤癌变组织间比较差异均无统计学意义(P＞0.05),伴CSM的大肠腺瘤与不伴CSM的大肠腺瘤比较差异均有统计学意义(P＜0.05),正常大肠黏膜与伴CSM的大肠腺瘤、大肠腺瘤癌变组织间比较差异均有统计学意义(P＜0.05).结论 伴CSM的大肠腺瘤具有有别于不伴有CSM的大肠腺瘤的生物学特性,其细胞增殖活跃而调亡受抑制,易发生癌变.
目的 研究伴鷄皮樣黏膜( CSM)改變的成人大腸腺瘤生物學特性及其臨床意義.方法 採用免疫組化SP法及酶聯免疫吸附試驗(ELISA)檢測正常大腸黏膜、伴CSM的大腸腺瘤、不伴CSM的大腸腺瘤和大腸腺瘤癌變標本中代錶細胞增殖的標誌物Ki-67、COX-2和細胞凋亡相關因子survivin、caspase-3蛋白的錶達情況.結果 免疫組化結果提示在大腸腺瘤癌變組織、伴CSM的大腸腺瘤、不伴CSM的大腸腺瘤及正常大腸黏膜中Ki-67、COX-2的錶達呈遞減趨勢,而survivin和caspase-3的錶達呈遞增趨勢.蛋白質定量分析(ELISA法)結果 提示Ki-67、COX-2、survivin、caspase-3含量在伴CSM的大腸腺瘤與腺瘤癌變組織間比較差異均無統計學意義(P＞0.05),伴CSM的大腸腺瘤與不伴CSM的大腸腺瘤比較差異均有統計學意義(P＜0.05),正常大腸黏膜與伴CSM的大腸腺瘤、大腸腺瘤癌變組織間比較差異均有統計學意義(P＜0.05).結論 伴CSM的大腸腺瘤具有有彆于不伴有CSM的大腸腺瘤的生物學特性,其細胞增殖活躍而調亡受抑製,易髮生癌變.
목적 연구반계피양점막( CSM)개변적성인대장선류생물학특성급기림상의의.방법 채용면역조화SP법급매련면역흡부시험(ELISA)검측정상대장점막、반CSM적대장선류、불반CSM적대장선류화대장선류암변표본중대표세포증식적표지물Ki-67、COX-2화세포조망상관인자survivin、caspase-3단백적표체정황.결과 면역조화결과제시재대장선류암변조직、반CSM적대장선류、불반CSM적대장선류급정상대장점막중Ki-67、COX-2적표체정체감추세,이survivin화caspase-3적표체정체증추세.단백질정량분석(ELISA법)결과 제시Ki-67、COX-2、survivin、caspase-3함량재반CSM적대장선류여선류암변조직간비교차이균무통계학의의(P＞0.05),반CSM적대장선류여불반CSM적대장선류비교차이균유통계학의의(P＜0.05),정상대장점막여반CSM적대장선류、대장선류암변조직간비교차이균유통계학의의(P＜0.05).결론 반CSM적대장선류구유유별우불반유CSM적대장선류적생물학특성,기세포증식활약이조망수억제,역발생암변.
Objective To explore the biological features of colorectal adenoma with chicken-skin mucosa ( CSM ) and its clinical significance.Methods Expression of cell proliferation markers ( Ki-67 and COX-2) and apoptosis-related factors ( survivin and caspase-3) in normal colorectal mucosa,colorectal adenoma without CSM,colorectal adenoma with CSM and colorectal adenocarcinoma were detected by immunohistochemistry SP method and enzyme-linked immunosorbent assay (ELISA).Results Immunohistochemical results revealed a decreased trend in expressions of Ki-67 and COX-2 from colorectal adenocarcinoma through colorectal adenoma with and without CSM to normal colorectal mucosa,while the expressions of survivin and caspase-3 showed an increased trend.By ELISA,the expressions of Ki-67,COX-2,surviving and caspase-3 showed no significant difference (P ＞ 0.05 ) between colorectal adenoma with CSM and coloreetal adenocarcima,while these variables were significantly different (P ＜ 0.05) between coloreetal adenoma with and without CSM,so as well between normal colorectal mucosa and other 3 groups.Conclusion The biological characteristics of colorectal adenoma with CSM are different from those without,showing an activated cell proliferation and inhibited cell apoptosis with increased carcinogenesis risk.
