CAJ | 학술논문

目的探讨缺氧/复氧对小鼠骨髓树突状细胞(DC)表型及生物学活性的影响.方法培养小鼠骨髓源性DC,分为对照组及缺氧/复氧组,对照组在正常培养条件下培养,缺氧/复氧组给予缺氧气体培养4 h,然后在正常培养条件下继续培养1 d.应用流式细胞仪检测DC表面CD80、CD86、MHC Ⅱ分子的变化,ELISA法检测DC分泌TNF-α、IFN-γ和IL-12的浓度,混合淋巴细胞培养检测T细胞增殖能力,免疫细胞化学检测Dc核因子-κB(NF-κB)的表达.结果缺氧/复氧可促进DC高表达CD80、CD86、MHC Ⅱ分子,促进Th1型细胞因子释放、NF-κB高表达及核移位,并诱导T细胞增殖.结论缺氧/复氧可刺激DC高表达表面分子,具有明显的免疫刺激活性.
목적 탐토결양/복양대소서골수수돌상세포(DC)표형급생물학활성적영향.방법 배양소서골수원성DC,분위대조조급결양/복양조,대조조재정상배양조건하배양,결양/복양조급여결양기체배양4 h,연후재정상배양조건하계속배양1 d.응용류식세포의검측DC표면CD80、CD86、MHC Ⅱ분자적변화,ELISA법검측DC분비TNF-α、IFN-γ화IL-12적농도,혼합림파세포배양검측T세포증식능력,면역세포화학검측Dc핵인자-κB(NF-κB)적표체.결과 결양/복양가촉진DC고표체CD80、CD86、MHC Ⅱ분자,촉진Th1형세포인자석방、NF-κB고표체급핵이위,병유도T세포증식.결론 결양/복양가자격DC고표체표면분자,구유명현적면역자격활성.
Objective To explore the impact of hypoxia/reoxygenation stimulation on phenotype and immune activity of dendritic cells(DCs) cultured from murine bone marrow. Methods Mouse DCs were generated from bone marrow cells and were divided into control group and hypoxia/reoxygenation group. DC in control group was cultured at normal condition, and in hypoxia/reoxygenation group was cultured at hypoxic condition for 4 h followed by cultured at normal condition for 24 h. Flow cytometry and mixed lym-phocyte reaction(MLR) was used to detect the phenotype and functional properties of DCs. ELISA was used to detect the concentration of TNF-α, IFN-γ and IL-12 in the supernalant, Imrounochemistry was used to de-tect the concentration of NF-κB. Results Hypoxia/reoxygen stimulation increased the CD80, CD86, MHC Ⅱ in the cytomembrane of DCs and TNF-α, IFN-γ, IL-12 concentration in the supernalant. Hypoxia/reoxy-gen stimulation also promoted the shift of NF-κB to karyon. Conclusion Under hypoxia/reoxygen stimula-tion, DCs express high level of surface molecules, and possess strong immune activity.