中国临床康复
中國臨床康複
중국림상강복
CHINESE JOURNAL OF CLINICAL REHABILITATION
2005年
3期
229-231
,共3页
背景:电磁辐射在应用到许多领域的同时,对人体健康也构成一定的威胁.男性生殖系统也是电磁辐射的敏感靶器官,睾酮在电磁辐射条件下的变化及其机制目前还不清楚. 目的:探讨电磁辐射对成年大鼠睾丸组织中类固醇合成急性调节蛋白( StAR)、细胞色素 P450胆固醇侧链裂解酶 (P450scc)的作用,初步揭示电磁辐射影响睾酮合成的分子机制. 设计:随机对照研究. 单位:解放军第三军医大学大坪医院野战外科研究所核医学科和劳动卫生学教研室. 材料:本研究于 2003- 06/2004- 05在解放军第三军医大学大坪医院野战外科研究所核医学科和劳动卫生学教研室完成.选择二级清洁级雄性成年 Wistar大鼠 75只,随机分为 5组,即对照组以及电磁辐射后 3,6,24,72 h组,每组 15只,体质量 180～ 220 g. 方法:采用峰值功率为 90 W/cm2的微波连续辐照 15 min,辐照完毕后,动物均存活.采用放射免疫方法( RIA)分别测定电磁辐照后 3,6,24,72 h及对照组血清睾酮含量,同时运用 RT-PCR方法测定睾丸组织中 StAR、 P450scc mRNA水平. 主要观察指标:电磁辐照后 3, 6, 24, 72 h组与对照组血清睾酮含量和 StAR,P450scc mRNA的水平. 结果:辐照后 3 h血清睾酮含量和 StAR 、 P450scc mRNA水平均明显低于对照组,分别较对照组降低 83.1%, 57.3%, 53.6%( P< 0.01); 6 h略有回升,但仍明显低于对照组,分别较对照组降低 52.6%, 17.9%, 29.2%( P< 0.01,0.05); 24 h恢复到正常水平; 72 h再次出现明显降低,分别较对照组降低 57.6%, 39.5%, 53.5%( P< 0.01). 结论:电磁辐射能在转录水平影响睾丸间质细胞 StAR 、 P450scc mRNA的表达,从而可能降低睾酮的合成.
揹景:電磁輻射在應用到許多領域的同時,對人體健康也構成一定的威脅.男性生殖繫統也是電磁輻射的敏感靶器官,睪酮在電磁輻射條件下的變化及其機製目前還不清楚. 目的:探討電磁輻射對成年大鼠睪汍組織中類固醇閤成急性調節蛋白( StAR)、細胞色素 P450膽固醇側鏈裂解酶 (P450scc)的作用,初步揭示電磁輻射影響睪酮閤成的分子機製. 設計:隨機對照研究. 單位:解放軍第三軍醫大學大坪醫院野戰外科研究所覈醫學科和勞動衛生學教研室. 材料:本研究于 2003- 06/2004- 05在解放軍第三軍醫大學大坪醫院野戰外科研究所覈醫學科和勞動衛生學教研室完成.選擇二級清潔級雄性成年 Wistar大鼠 75隻,隨機分為 5組,即對照組以及電磁輻射後 3,6,24,72 h組,每組 15隻,體質量 180～ 220 g. 方法:採用峰值功率為 90 W/cm2的微波連續輻照 15 min,輻照完畢後,動物均存活.採用放射免疫方法( RIA)分彆測定電磁輻照後 3,6,24,72 h及對照組血清睪酮含量,同時運用 RT-PCR方法測定睪汍組織中 StAR、 P450scc mRNA水平. 主要觀察指標:電磁輻照後 3, 6, 24, 72 h組與對照組血清睪酮含量和 StAR,P450scc mRNA的水平. 結果:輻照後 3 h血清睪酮含量和 StAR 、 P450scc mRNA水平均明顯低于對照組,分彆較對照組降低 83.1%, 57.3%, 53.6%( P< 0.01); 6 h略有迴升,但仍明顯低于對照組,分彆較對照組降低 52.6%, 17.9%, 29.2%( P< 0.01,0.05); 24 h恢複到正常水平; 72 h再次齣現明顯降低,分彆較對照組降低 57.6%, 39.5%, 53.5%( P< 0.01). 結論:電磁輻射能在轉錄水平影響睪汍間質細胞 StAR 、 P450scc mRNA的錶達,從而可能降低睪酮的閤成.
배경:전자복사재응용도허다영역적동시,대인체건강야구성일정적위협.남성생식계통야시전자복사적민감파기관,고동재전자복사조건하적변화급기궤제목전환불청초. 목적:탐토전자복사대성년대서고환조직중류고순합성급성조절단백( StAR)、세포색소 P450담고순측련렬해매 (P450scc)적작용,초보게시전자복사영향고동합성적분자궤제. 설계:수궤대조연구. 단위:해방군제삼군의대학대평의원야전외과연구소핵의학과화노동위생학교연실. 재료:본연구우 2003- 06/2004- 05재해방군제삼군의대학대평의원야전외과연구소핵의학과화노동위생학교연실완성.선택이급청길급웅성성년 Wistar대서 75지,수궤분위 5조,즉대조조이급전자복사후 3,6,24,72 h조,매조 15지,체질량 180～ 220 g. 방법:채용봉치공솔위 90 W/cm2적미파련속복조 15 min,복조완필후,동물균존활.채용방사면역방법( RIA)분별측정전자복조후 3,6,24,72 h급대조조혈청고동함량,동시운용 RT-PCR방법측정고환조직중 StAR、 P450scc mRNA수평. 주요관찰지표:전자복조후 3, 6, 24, 72 h조여대조조혈청고동함량화 StAR,P450scc mRNA적수평. 결과:복조후 3 h혈청고동함량화 StAR 、 P450scc mRNA수평균명현저우대조조,분별교대조조강저 83.1%, 57.3%, 53.6%( P< 0.01); 6 h략유회승,단잉명현저우대조조,분별교대조조강저 52.6%, 17.9%, 29.2%( P< 0.01,0.05); 24 h회복도정상수평; 72 h재차출현명현강저,분별교대조조강저 57.6%, 39.5%, 53.5%( P< 0.01). 결론:전자복사능재전록수평영향고환간질세포 StAR 、 P450scc mRNA적표체,종이가능강저고동적합성.
BACKGROUND:With the development of technology,electromagnetic irradiation has been applied in many fields;meanwhile,health of people could be threatened by electromagnetic exposure.Reproductive system of males is the major target of electromagnetic irradiation.But under the condition of electromagnetic exposure,the change of testosterone and its mechanism remain unknown. OBJECTIVE:To study the effect of electromagnetic irradiation on steroidogenic acute regulatory protein(StAR) and cytochrome P450 cholesterol side-chain cleavage(P450scc) in the tissues of the testis of the adult rats,and to preliminarily reveal the molecular mechanism of electromagnetic radiation that affects the synthesis of testosterone. DESIGN:A randomized controlled study. SETTING:Department of Nuclear Medicine and Staff Room of Labour Hygiene, Institute of Field Surgery,Daping Hospital of the Third Military Medical University of Chinese PLA. PARTICIPANTS:The study was completed in the Department of Nuclear Medicine and Staff Room of Labour Hygiene, Institute of Field Surgery,Daping Hospital of the Third Military Medical University of Chinese PLA from June 2003 to May 2004.A total of 75 male Wistar rats of clean degree II,weighing 180 to 220 g,were randomly divided into 5 groups:control group,and groups of 3,6,24,72 hours after electromagnetic irradiation with 15 rats in each group. INTERVENTIONS:The rats were exposed to microwave with the peak power of 90 W/cm2 for 15 minutes successively,and all the rats survived the irradiation.The serum contents of testosterone of rats in the groups of 3,6,24,72 hours after electromagnetic irradiation and control group were measured by using radioimmunoassay(RIA) respectively;meanwhile the levels of the StAR and P450scc mRNA in testis tissue were also determined with RT-PCR. MAIN OUTCOME MEASURES:Serum contents of testosterone in the groups of 3,6,24,72 hours after electromagnetic irradiation and control group;the expression levels of the StAR and P450scc mRNA in testis tissues. RESULTS:The serum contents of testosterone,the StAR and P450scc mRNA levels at the point of 3 hours after electromagnetic irradiation were significantly lower than those in the control group,and they were decreased by 83.1% ,57.3% and 53.6% respectively(P< 0.01).At the point of 6 hours, although those were increased a little, but were still obviously lower than those of the control group, and they were decreased by 52.6%, 17.9% and 29.2% respectively(P< 0.01,0.05) compared with the control group, and were recovered to the normal level at the point of 24 hours, but the contents of testosterone, the StAR and P450scc mRNA levels were significantly reduced again after 72 hours, and they were decreased by 57.6% , 39.5% , 53.5% respectively compared with those in the control group(P< 0.01). CONCLUSION:Electromagnetic irradiation can affect the expression of StAR and P450scc mRNA in the Leydig's cells of the adult rats, thereby decreasing the synthesis of testosterone.
