CAJ | 학술논문

透射和扫描电镜研究显示玫瑰无须鲃(Puntius conchonius)的精子由头、中片和尾三部分组成.头部无顶体,呈球形或卵圆形,主要由细胞核组成,核内染色质致密.核前端几乎无细胞质存在,核膜紧密靠近细胞质膜,而在核的后端有少量细胞质存在.在核后端偏于一侧处有一个浅的核后凹,中心粒复合体部分地镶嵌于其中,中心粒复合体由近端中心粒和远端中心粒组成,二者呈钝角形排列,鞭毛从远端中心粒的末端发出.中片由前边的主要部分--领和后边细薄的袖套构成.领内含有数个不规则分布的线粒体包埋于细胞质中,袖套的长短、粗细差别较大,有的精子没有袖套.由于与鞭毛的不对称连接,使得头部及中片均呈不对称型.尾是一根细长的鞭毛,尾丝具有典型的"9+2"微管结构,尾部两侧均无侧鳍.与鲤科其它鱼精子相比,该鱼精子的主要特征是具有长短不一的袖套,领内有不同数量的液泡,且有些空泡向外界开口呈孔状.袖套的长短与领内液泡化水平似有某种相互联系,这也许与精子的老化程度有关
투사화소묘전경연구현시매괴무수파(Puntius conchonius)적정자유두、중편화미삼부분조성.두부무정체,정구형혹란원형,주요유세포핵조성,핵내염색질치밀.핵전단궤호무세포질존재,핵막긴밀고근세포질막,이재핵적후단유소량세포질존재.재핵후단편우일측처유일개천적핵후요,중심립복합체부분지양감우기중,중심립복합체유근단중심립화원단중심립조성,이자정둔각형배렬,편모종원단중심립적말단발출.중편유전변적주요부분--령화후변세박적수투구성.령내함유수개불규칙분포적선립체포매우세포질중,수투적장단、조세차별교대,유적정자몰유수투.유우여편모적불대칭련접,사득두부급중편균정불대칭형.미시일근세장적편모,미사구유전형적"9+2"미관결구,미부량측균무측기.여리과기타어정자상비,해어정자적주요특정시구유장단불일적수투,령내유불동수량적액포,차유사공포향외계개구정공상.수투적장단여령내액포화수평사유모충상호련계,저야허여정자적노화정도유관
Transmission and scanning electron microscopy revealed that the spermatozoon of the rosy barb Puntius conchonius consists of an acrosome-less ovoid head,a midpiece and a flagellum.The head mainly consists of the nucleus.There is only a thin layer of cytoplasm between the plasmalemma and the nucleus.The nucleus contains dense chromatin.At the caudal-lateral end of the nucleus there is a shallow nuclear fossa,which partly holds the centriolar complex.The centriolar complex consists of the proximal and distal centrioles,arranged at an obtuse angle with the flagellum linked to the end of the distal centriole.The midpiece is composed of the anterior chief segment,the collar,containing several irregular mitochondria embedded in the cytoplasmic mass,and a slender caudal segment of variable length,the sleeve,behind the collar.The midpiece is separated from the anterior segment of the flagellum by a plasmic channel.Both the head and the midpiece are in asymmetrical emplacement to the position of the flagellum.The flagellum possesses a conventional 9+2 axoneme with no lateral fin.Compared to the structures of other Cyprinidae fish spermatozoa,the main characteristics of this spermatozoon are the variable length of the sleeve,the different vesicles and various pores of the collar.It seems that the number of vesicles and the sleeve length is correlated,this may have some relationship with sperm aging