中国临床康复
中國臨床康複
중국림상강복
CHINESE JOURNAL OF CLINICAL REHABILITATION
2005年
15期
234-236
,共3页
刘志诚%孙凤岷%袁锦虹%姜军作%衣运玲%吕雅妮
劉誌誠%孫鳳岷%袁錦虹%薑軍作%衣運玲%呂雅妮
류지성%손봉민%원금홍%강군작%의운령%려아니
肥胖症/病理生理学%弓状核/针灸效应%体重%大鼠%针刺疗法
肥胖癥/病理生理學%弓狀覈/針灸效應%體重%大鼠%針刺療法
비반증/병리생이학%궁상핵/침구효응%체중%대서%침자요법
背景:弓状核功能异常可能是肥胖发病的重要因素之一,已知针灸减肥机制涉及神经及神经体液调节,针刺对弓状核功能调整作用如何?目的:探讨针刺对肥胖大鼠弓状核功能的作用,进一步深化针刺减肥的中枢神经作用机制.设计:以实验动物为研究对象的随机对照实验研究.单位:一所中医药大学临床医学院的针灸研究所及一所人口管理学院.材料:实验于2002-04/10在南京中医药大学第二临床医学院针灸研究所完成.选用1个月龄刚断乳SD雄性大鼠.方法:以普通全价鼠饲料喂养的大鼠作为正常组,将造模成功的实验性肥胖大鼠随机分为对照组和针刺组,每组12只.针刺组大鼠给予针刺治疗14 d,正常组和对照组大鼠分别每天放入大鼠固定器中适应15 min,持续14 d.采用神经电生理及神经化学技术观察针刺治疗前后肥胖大鼠体质量、Lee's指数、体脂,中枢和外周瘦素和胰岛素水平以及下丘脑弓状核神经细胞自发放电频率的变化.主要观察指标:①针刺对实验性肥胖大鼠肥胖指标及对心包、肾周和附睾脂肪量的影响.②针刺对各组大鼠下丘脑弓状核神经细胞自发放电的影响.③针刺对各组大鼠下丘脑和血清瘦素和胰岛素含量的影响.④下丘脑弓状核神经细胞自发放电与下丘脑瘦素和胰岛素水平的相关分析.结果:肥胖大鼠体质量、Lee's指数、体脂量[(517.74±29.35)g,(319.85±3.96),(13.88±1.32)g,P＜0.01]均显著高于正常大鼠水平;肥胖大鼠下丘脑弓状核神经细胞自发放电频率显著低于正常大鼠[(3.12±1.92)Hz,(8.99±2.71)Hz,P＜0.01];大鼠下丘脑弓状核神经细胞自发放电频率与体质量、Lee's指数和体脂量均呈负相关(r=-0.592,-0.672,-0.521).针刺组较对照组肥胖大鼠下丘脑弓状核神经细胞自发放电频率明显增高[(9.75±2.02)Hz,P＜0.01].结论:针刺对肥胖机体下丘脑弓状核功能的良性作用可能是针灸减肥的作用机制之一.
揹景:弓狀覈功能異常可能是肥胖髮病的重要因素之一,已知針灸減肥機製涉及神經及神經體液調節,針刺對弓狀覈功能調整作用如何?目的:探討針刺對肥胖大鼠弓狀覈功能的作用,進一步深化針刺減肥的中樞神經作用機製.設計:以實驗動物為研究對象的隨機對照實驗研究.單位:一所中醫藥大學臨床醫學院的針灸研究所及一所人口管理學院.材料:實驗于2002-04/10在南京中醫藥大學第二臨床醫學院針灸研究所完成.選用1箇月齡剛斷乳SD雄性大鼠.方法:以普通全價鼠飼料餵養的大鼠作為正常組,將造模成功的實驗性肥胖大鼠隨機分為對照組和針刺組,每組12隻.針刺組大鼠給予針刺治療14 d,正常組和對照組大鼠分彆每天放入大鼠固定器中適應15 min,持續14 d.採用神經電生理及神經化學技術觀察針刺治療前後肥胖大鼠體質量、Lee's指數、體脂,中樞和外週瘦素和胰島素水平以及下丘腦弓狀覈神經細胞自髮放電頻率的變化.主要觀察指標:①針刺對實驗性肥胖大鼠肥胖指標及對心包、腎週和附睪脂肪量的影響.②針刺對各組大鼠下丘腦弓狀覈神經細胞自髮放電的影響.③針刺對各組大鼠下丘腦和血清瘦素和胰島素含量的影響.④下丘腦弓狀覈神經細胞自髮放電與下丘腦瘦素和胰島素水平的相關分析.結果:肥胖大鼠體質量、Lee's指數、體脂量[(517.74±29.35)g,(319.85±3.96),(13.88±1.32)g,P＜0.01]均顯著高于正常大鼠水平;肥胖大鼠下丘腦弓狀覈神經細胞自髮放電頻率顯著低于正常大鼠[(3.12±1.92)Hz,(8.99±2.71)Hz,P＜0.01];大鼠下丘腦弓狀覈神經細胞自髮放電頻率與體質量、Lee's指數和體脂量均呈負相關(r=-0.592,-0.672,-0.521).針刺組較對照組肥胖大鼠下丘腦弓狀覈神經細胞自髮放電頻率明顯增高[(9.75±2.02)Hz,P＜0.01].結論:針刺對肥胖機體下丘腦弓狀覈功能的良性作用可能是針灸減肥的作用機製之一.
배경:궁상핵공능이상가능시비반발병적중요인소지일,이지침구감비궤제섭급신경급신경체액조절,침자대궁상핵공능조정작용여하?목적:탐토침자대비반대서궁상핵공능적작용,진일보심화침자감비적중추신경작용궤제.설계:이실험동물위연구대상적수궤대조실험연구.단위:일소중의약대학림상의학원적침구연구소급일소인구관이학원.재료:실험우2002-04/10재남경중의약대학제이림상의학원침구연구소완성.선용1개월령강단유SD웅성대서.방법:이보통전개서사료위양적대서작위정상조,장조모성공적실험성비반대서수궤분위대조조화침자조,매조12지.침자조대서급여침자치료14 d,정상조화대조조대서분별매천방입대서고정기중괄응15 min,지속14 d.채용신경전생리급신경화학기술관찰침자치료전후비반대서체질량、Lee's지수、체지,중추화외주수소화이도소수평이급하구뇌궁상핵신경세포자발방전빈솔적변화.주요관찰지표:①침자대실험성비반대서비반지표급대심포、신주화부고지방량적영향.②침자대각조대서하구뇌궁상핵신경세포자발방전적영향.③침자대각조대서하구뇌화혈청수소화이도소함량적영향.④하구뇌궁상핵신경세포자발방전여하구뇌수소화이도소수평적상관분석.결과:비반대서체질량、Lee's지수、체지량[(517.74±29.35)g,(319.85±3.96),(13.88±1.32)g,P＜0.01]균현저고우정상대서수평;비반대서하구뇌궁상핵신경세포자발방전빈솔현저저우정상대서[(3.12±1.92)Hz,(8.99±2.71)Hz,P＜0.01];대서하구뇌궁상핵신경세포자발방전빈솔여체질량、Lee's지수화체지량균정부상관(r=-0.592,-0.672,-0.521).침자조교대조조비반대서하구뇌궁상핵신경세포자발방전빈솔명현증고[(9.75±2.02)Hz,P＜0.01].결론:침자대비반궤체하구뇌궁상핵공능적량성작용가능시침구감비적작용궤제지일.
BACKGROUND: The abnormality of the function of arcuate nucleus may be an important factor of obesity. It has been known that the mechanisms of acupuncture in treating obesity are related to nervous and neurohumoral regulation. What is the regulating effect of acupuncture on the function of arcuate nucleus?OBJECTIVE: To study the effects of acupuncture on the function of arcuate nucleus of obese rats, and further investigate central nervous functional mechanism of reducing weight by acupuncture.DESIGN: Randomized controlled study based on the experimental animals.SETTING: Acupuncture institute in second clinical medical college of a university of traditional Chinese medicine, and a population management college.MATERIALS: This experiment was carried out in the Acupuncture Institute of Second Clinical Medical College, Nanjing University of Traditional Chinese Medicine between April and October 2002. One-month old male SD rats just in ablactation were selected.METHODS: Rats fed with ordinary wholesome rat-feed were in the normal group. The successfully established experimental obese rats models were randomly divided as control group and acupuncture group with 12 rats in each group. Rats in the acupuncture group were given acupuncture treatment for 14 days, and rats in the normal and control groups were put into rat fixation-machine for 15 minutes every day, lasting for 14 days. Body mass, Lee' s index, body lipid, level of central and peripheral leptin and insulin(INS) as well as the frequency of spontaneous discharge of nerve cell in the arcuate nucleus(ARC) of hypothalamus in obese rats were observed with nervous electrophysiological and nervous biochemical technology before and after acupuncture.MAIN OUTCOME MEASURES: ① Effect of acupuncture on obesity index, fat contents of pericardium, kidney and epididymis of experimental obese rats. ② Effect of acupuncture on the frequency of spontaneous discharge of ARC nerve cell of hypothalamus in obese rats. ③ Effect of acupuncture on the contents of hypothalamic and serum leptin and INS of rats in each groups. ④ Correlation of the frequency of spontaneous discharge of ARC nerve cell of hypothalamus with the level of hypothalamic leptin and INS.RESULTS: The body mass[(517.74±29.35) g], Lee' s index (319.85 ± 3.96) and body fat content of obesity rats [ (13.88 ± 1.32) g] were all significantly higher than those in the normal rats, the differences were significant(P ＜ 0.01); the frequency of spontaneous discharge of nerve cell in ARC of hypothalamus in obese rats [ (3.12 ± 1.92) Hz] were all markedly lower than those of the normal rats[ (8.99 ± 2.71 ) Hz], the differences were significant (P ＜ 0.01 ); the frequency of spontaneous discharge of nerve cell in ARC was negatively correlated with body mass, Lee' s index and body lipid( r = -0. 592, -0. 672, -0. 521 ) . Acupuncture significantly increased the frequency of spontaneous discharge of nerve cell in ARC of hypothalamus in obese rats [(9.75 ±2.02) Hz] as compared with the control group, the differences were significant( P ＜ 0. 01 ).CONCLUSION: The good regulating effect of acupuncture on the function of arcuate nucleus of hypothalamus might be one of mechanisms in the antiobesity of acupuncture.
