干旱地区农业研究
榦旱地區農業研究
간한지구농업연구
AGRICULTURAL RESEARCH IN THE ARID AREAS
2009年
4期
99-104
,共6页
王俪梅%李青松%周春菊%尚浩博%王林权
王儷梅%李青鬆%週春菊%尚浩博%王林權
왕려매%리청송%주춘국%상호박%왕림권
盐胁迫%小麦%SOD%游离脯氨酸
鹽脅迫%小麥%SOD%遊離脯氨痠
염협박%소맥%SOD%유리포안산
salt stress%wheat%SOD%MDA%free proline
以4种不同基因型小麦(小偃6号、NR9405、RB6和陕229)种子为材料,研究组培条件下盐胁迫对小麦种子活力、幼苗生物量、保护酶活性、亲合性渗调物质以及Na+、K+ 含量等的影响.结果表明:盐胁迫下,小麦幼苗的生长均受到不同程度的抑制,表现为发芽率、根长、芽长、生物量随着盐浓度的升高而明显下降,并存在显著的基因型差异.盐胁迫下小偃6号与NR9405的生物量显著高于陕229与RB6;小偃6号与NR9405的超氧化物歧化酶(SOD )活性均随着盐浓度的增大而升高;RB6和陕229在0~150 mmol/L盐浓度下SOD酶活性升高,而在2 00 mmol/L盐浓度时,SOD活性急剧下降.随着盐浓度的升高4种不同基因型小麦体内游离脯氨酸累积量增大,在200 mmol/L时RB6的累积量显著高于其它基因型.随着盐浓度的升高,幼苗体内的Na+含量增加,K+含量下降,Na+/K+比值增大.小偃6号和NR9405的耐盐性优于陕229与RB6.
以4種不同基因型小麥(小偃6號、NR9405、RB6和陝229)種子為材料,研究組培條件下鹽脅迫對小麥種子活力、幼苗生物量、保護酶活性、親閤性滲調物質以及Na+、K+ 含量等的影響.結果錶明:鹽脅迫下,小麥幼苗的生長均受到不同程度的抑製,錶現為髮芽率、根長、芽長、生物量隨著鹽濃度的升高而明顯下降,併存在顯著的基因型差異.鹽脅迫下小偃6號與NR9405的生物量顯著高于陝229與RB6;小偃6號與NR9405的超氧化物歧化酶(SOD )活性均隨著鹽濃度的增大而升高;RB6和陝229在0~150 mmol/L鹽濃度下SOD酶活性升高,而在2 00 mmol/L鹽濃度時,SOD活性急劇下降.隨著鹽濃度的升高4種不同基因型小麥體內遊離脯氨痠纍積量增大,在200 mmol/L時RB6的纍積量顯著高于其它基因型.隨著鹽濃度的升高,幼苗體內的Na+含量增加,K+含量下降,Na+/K+比值增大.小偃6號和NR9405的耐鹽性優于陝229與RB6.
이4충불동기인형소맥(소언6호、NR9405、RB6화협229)충자위재료,연구조배조건하염협박대소맥충자활력、유묘생물량、보호매활성、친합성삼조물질이급Na+、K+ 함량등적영향.결과표명:염협박하,소맥유묘적생장균수도불동정도적억제,표현위발아솔、근장、아장、생물량수착염농도적승고이명현하강,병존재현저적기인형차이.염협박하소언6호여NR9405적생물량현저고우협229여RB6;소언6호여NR9405적초양화물기화매(SOD )활성균수착염농도적증대이승고;RB6화협229재0~150 mmol/L염농도하SOD매활성승고,이재2 00 mmol/L염농도시,SOD활성급극하강.수착염농도적승고4충불동기인형소맥체내유리포안산루적량증대,재200 mmol/L시RB6적루적량현저고우기타기인형.수착염농도적승고,유묘체내적Na+함량증가,K+함량하강,Na+/K+비치증대.소언6호화NR9405적내염성우우협229여RB6.
The seed vigor, seedling biomass, protective enzyme activity and compatible os m olytes as well as Na+ and K+ content of four wheat genotypes seedling under salt stress were studied in vitro. The results indicated that the seed germinati on and seedling growth were inhibited under the salt stress. The germination rat e, single root length, bud length and biomass decreased significantly as the inc rease of salt concentration, and there were significant differences among genoty pes. The biomass of Xiaoyan 6 and NR9405 were significantly greater than Sha an229 and RB6 under salt stress. SOD activity of Xiaoyan and NR9405 increas ed with the increase of salt concentration. SOD activity of RB6 and Shaan229 inc reased under the salt concentration of 0~150 mmol/L; however, it decreased shar ply under the 200 mmol/L salt concentration. As the increase of salt concent r ation, the free proline content in four different wheat genotypes increased, and the accumulation in RB6 was significantly higher than others under the 200 mmol /L salt concentration. The Na+ content and Na+/K+ in wheat seedlings rose but K+ content dropped owing to the increase of salt concentration. Overall, X iaoyan6 and NR9405 were better than Shaan229 and RB6 in salt tolerance.