南方医科大学学报
南方醫科大學學報
남방의과대학학보
JOURNAL OF SOUTHERN MEDICAL UNIVERSITY
2009年
11期
2194-2196
,共3页
环氧化酶-2%塞来昔布%癌%鳞状细胞%舌肿瘤%细胞迁移
環氧化酶-2%塞來昔佈%癌%鱗狀細胞%舌腫瘤%細胞遷移
배양화매-2%새래석포%암%린상세포%설종류%세포천이
COX-2%celecoxib%carcinoma%squamous cell%tongue neoplasms%cell migration
目的 研究环氧化酶-2(COX-2)抑制剂塞来昔布(Celecoxib)抑制人舌鳞癌Tca8113细胞迁移的作用.方法 采用细胞划痕实验、细胞.基质黏附实验和Boyden趋化小室测定Tca8113细胞的迁移能力.观察塞来昔布对Tca8113细胞迁移作用的影响.结果 10 μmol/L和20 μmol/L塞来昔布处理细胞24 h后.细胞迁移数与对照组相比减少有统计学意义(P<0.05),Tca8113细胞的迁移能力显著减弱.不同浓度塞来昔布处理Tca8113细胞24 h后,其与包被Fn基质胶表面上的黏附情况呈剂量依赖天系,随塞来昔布浓度的增加,Tca8113细胞在基质表面的黏附显著降低.结论 COX-2抑制剂塞来昔布具有抑制Tca8113细胞迁移的能力,其机制有待进一步研究.
目的 研究環氧化酶-2(COX-2)抑製劑塞來昔佈(Celecoxib)抑製人舌鱗癌Tca8113細胞遷移的作用.方法 採用細胞劃痕實驗、細胞.基質黏附實驗和Boyden趨化小室測定Tca8113細胞的遷移能力.觀察塞來昔佈對Tca8113細胞遷移作用的影響.結果 10 μmol/L和20 μmol/L塞來昔佈處理細胞24 h後.細胞遷移數與對照組相比減少有統計學意義(P<0.05),Tca8113細胞的遷移能力顯著減弱.不同濃度塞來昔佈處理Tca8113細胞24 h後,其與包被Fn基質膠錶麵上的黏附情況呈劑量依賴天繫,隨塞來昔佈濃度的增加,Tca8113細胞在基質錶麵的黏附顯著降低.結論 COX-2抑製劑塞來昔佈具有抑製Tca8113細胞遷移的能力,其機製有待進一步研究.
목적 연구배양화매-2(COX-2)억제제새래석포(Celecoxib)억제인설린암Tca8113세포천이적작용.방법 채용세포화흔실험、세포.기질점부실험화Boyden추화소실측정Tca8113세포적천이능력.관찰새래석포대Tca8113세포천이작용적영향.결과 10 μmol/L화20 μmol/L새래석포처리세포24 h후.세포천이수여대조조상비감소유통계학의의(P<0.05),Tca8113세포적천이능력현저감약.불동농도새래석포처리Tca8113세포24 h후,기여포피Fn기질효표면상적점부정황정제량의뢰천계,수새래석포농도적증가,Tca8113세포재기질표면적점부현저강저.결론 COX-2억제제새래석포구유억제Tca8113세포천이적능력,기궤제유대진일보연구.
Objective To investigate the role of COX-2 inhibitor celecoxib in inhibiting the migration of human tongue squamous cell carcinoma Tca8113 cells. Methods The effects of celecoxib on Tca8113 cell migration were tested using scrape motility assay, cell-matrix adhesion assay and Boyden chamber motility assay. Results Following a 24-hour incubation with 10 and 20 μmol/L celecoxib, the migration of Tca8113 cells was significantly decreased (P<0.05). Celecoxib treatment for 24 h also resulted in significantly decreased adhesion of Tca8113 cells on Fn-coated surface in a dose-dependent manner. Conclusion COX-2 inhibitor celecoxib can inhibit Tca8113 cell migration, the mechanism of which awaits further investigation.
