临床耳鼻咽喉头颈外科杂志
臨床耳鼻嚥喉頭頸外科雜誌
림상이비인후두경외과잡지
JOURNAL OF CLINICAL OTORHINOLARYNGOLOGY HEAD AND NECK SURGERY
2009年
22期
1038-1043
,共6页
膜片钳制技术%离子通道%耳蜗螺旋神经节细胞%电生理学%小鼠
膜片鉗製技術%離子通道%耳蝸螺鏇神經節細胞%電生理學%小鼠
막편겸제기술%리자통도%이와라선신경절세포%전생이학%소서
patch clamp techniques%ion channels%spiral ganglion neurons%electrophysiology%mice
目的:应用膜片钳技术记录小鼠耳蜗螺旋神经节细胞的全细胞电流,了解电压依赖性离子通道的基本电生理学特性,并比较耳蜗顶、底转螺旋神经节细胞电生理学特性的差异.方法:应用全细胞构型电压钳制技术,采用不同的电极内液及阻断剂,在不同的刺激参数下记录耳蜗顶、底转螺旋神经节细胞的电压依赖性离子通道电流,并进行分析比较.结果:实验记录到了内向的钠电流、延迟整流钾电流、超极化激活内向阳离子通道电流及瞬时外向钾电流,并发现耳蜗顶、底转螺旋神经节细胞的延迟整流钾电流及瞬时外向钾电流的电生理学特性具有显著性差异(P＜0.05).结论:实验记录到的各种离子电流数据表明耳蜗螺旋神经节细胞具有完成动作电位的形成、传导并对其功能进行调节的离子通道基础;耳蜗顶、底转螺旋神经节细胞电生理学特性的差异有助于听觉的形成过程.
目的:應用膜片鉗技術記錄小鼠耳蝸螺鏇神經節細胞的全細胞電流,瞭解電壓依賴性離子通道的基本電生理學特性,併比較耳蝸頂、底轉螺鏇神經節細胞電生理學特性的差異.方法:應用全細胞構型電壓鉗製技術,採用不同的電極內液及阻斷劑,在不同的刺激參數下記錄耳蝸頂、底轉螺鏇神經節細胞的電壓依賴性離子通道電流,併進行分析比較.結果:實驗記錄到瞭內嚮的鈉電流、延遲整流鉀電流、超極化激活內嚮暘離子通道電流及瞬時外嚮鉀電流,併髮現耳蝸頂、底轉螺鏇神經節細胞的延遲整流鉀電流及瞬時外嚮鉀電流的電生理學特性具有顯著性差異(P＜0.05).結論:實驗記錄到的各種離子電流數據錶明耳蝸螺鏇神經節細胞具有完成動作電位的形成、傳導併對其功能進行調節的離子通道基礎;耳蝸頂、底轉螺鏇神經節細胞電生理學特性的差異有助于聽覺的形成過程.
목적:응용막편겸기술기록소서이와라선신경절세포적전세포전류,료해전압의뢰성리자통도적기본전생이학특성,병비교이와정、저전라선신경절세포전생이학특성적차이.방법:응용전세포구형전압겸제기술,채용불동적전겁내액급조단제,재불동적자격삼수하기록이와정、저전라선신경절세포적전압의뢰성리자통도전류,병진행분석비교.결과:실험기록도료내향적납전류、연지정류갑전류、초겁화격활내향양리자통도전류급순시외향갑전류,병발현이와정、저전라선신경절세포적연지정류갑전류급순시외향갑전류적전생이학특성구유현저성차이(P＜0.05).결론:실험기록도적각충리자전류수거표명이와라선신경절세포구유완성동작전위적형성、전도병대기공능진행조절적리자통도기출;이와정、저전라선신경절세포전생이학특성적차이유조우은각적형성과정.
Objective: To explore the nature of voltage dependent ion channels and basic electrophysiological characteristics of cochlear spiral ganglion neurons of apical and basal turn by patch clamp techniques of whole cell configure on murine spiral ganglion neurons. Method: Different voltage dependent ionic currents were recorded with patch clamp techniques of whole cell configure on the condition of different internal electrode solution,blockers and stimulus protocol. Result: Inward sodium channel current(I_(Na)), hyperpolarization-activated inward cationic current(I_h) ,outward delay rectification potassium current (I_k) and outward transient potassium current (I_A)were recorded , significant difference of electrophysiological characteristics of I_A and I_k was found between apical and basal turns(P＜0.05). Conclusion: Various ionic currents are recorded,which shows that spiral ganglion neurons have the base of ionic channels to complete formation,conduction and modulation of action potential for auditory information transduction, the difference of electrophysiological characteristics between apical and basal turns contributes to the course of hearing formation.
