CAJ | 학술논문

[目的]研究重金属胁迫培养对2种细菌生长曲线的影响.[方法]以大肠杆菌和枯草芽孢杆菌2种典型细菌为试验材料,采用传统培养方法,选择5种重金属离子Cu2+、Hg2+、Pb2+、Cd2+、Cr6+在不同浓度下对其进行胁迫培养,通过测定2种细菌的生长曲线,研究外源性重金属对2种细菌生长的影响.[结果]Hg2+、Cd2+毒性较强,2种细菌的增殖在高浓度时受到抑制,G+比G-更为敏感;当重金属浓度＞50 mg/L后,5种重金属的对2种细菌的毒性顺序为Hg2+＞Cd2+＞Cu2+＞Cr6+≈Pb2+.[结论]该研究结果为进一步研究重金属对环境及生态系统的影响奠定基础.
[목적]연구중금속협박배양대2충세균생장곡선적영향.[방법]이대장간균화고초아포간균2충전형세균위시험재료,채용전통배양방법,선택5충중금속리자Cu2+、Hg2+、Pb2+、Cd2+、Cr6+재불동농도하대기진행협박배양,통과측정2충세균적생장곡선,연구외원성중금속대2충세균생장적영향.[결과]Hg2+、Cd2+독성교강,2충세균적증식재고농도시수도억제,G+비G-경위민감;당중금속농도＞50 mg/L후,5충중금속적대2충세균적독성순서위Hg2+＞Cd2+＞Cu2+＞Cr6+≈Pb2+.[결론]해연구결과위진일보연구중금속대배경급생태계통적영향전정기출.
[Objective] The aim was to study on the effects of heavy metal stress on the growth curves of Escherichia coli and Bacillus subtili. [Method] Using traditional culture method, Escherichia coli and Bacillus subtili were cultured under heavy metal stress including Cu2+, Hg2+, Pb2+, Cd2+ and Cr6+ in different concentrations. Then the growth curves of the bacteria were determined to investigate the effects of exogenous heavy metals on the growth of the two kinds of bacteria. [Result] The proliferation of the two bacteria was inhibited at high concentrations of Hg2+ and Cd2+ respectively, and G+ is more sensitive to them than G-; when the heavy metal concentration was >50 mg/L, the toxicity of the five kinds of heavy metals on the two bacteria was Hg2+＞Cd2+＞Cu2+＞Cr6+≈Pb2+. [Conclusion] The research will provide a basis to explore the effects of heavy metal on environment and ecological system.