中华消化外科杂志
中華消化外科雜誌
중화소화외과잡지
CHINESE JOURNAL OF DIGESTIVE SURGERY
2011年
5期
380-382
,共3页
李甫%范明明%杨超%王婷%张晞文
李甫%範明明%楊超%王婷%張晞文
리보%범명명%양초%왕정%장희문
Oddi括约肌%压力%一氧化氮%胆囊收缩素%犬
Oddi括約肌%壓力%一氧化氮%膽囊收縮素%犬
Oddi괄약기%압력%일양화담%담낭수축소%견
Sphincter of Oddi%Pressure%Nitric oxide%Cholecystokinin%Dogs
目的 探讨一氧化氮和胆囊收缩素(CCK)在犬Oddi括约肌运动调节中的作用.方法 测定正常状态及注射CCK、硝普钠、一氧化氮合酶(NOS)抑制剂N-硝基-L-精氨酸甲酯(L-NAME)时,犬Oddi括约肌的基础压、时相收缩频率、时相收缩幅度;免疫组织化学染色法检测犬Oddi括约肌上NOS阳性神经元的表达情况.计量资料的比较采用t检验.结果 注射0、20 ng/kg CCK后,犬Oddi括约肌基础压、时相收缩频率、时相收缩幅度分别为(27±10)mm Hg(1 mm Hg=0.133 kPa)、(10±3)次/min、(32±8)mm Hg和(61±14)mm Hg、(64±21)次/min、(44±15)mm Hg;注射100 ng/kg CCK后产生最大抑制作用和最大兴奋作用时,犬Oddi括约肌基础压、时相收缩频率、时相收缩幅度分别为(77 +31)mm Hg、(69±18)次/min、(79±14)mm Hg和(140±21)mm Hg、(129±25)次/min、( 173±63)mm Hg.胆总管滴注硝普钠后,犬Oddi括约肌基础压、时相收缩幅度均下降(t =3.706,5.183,P＜0.05).而注射L-NAME后,犬Oddi括约肌基础压、时相收缩幅度均升高(t=5.859,3.588,P＜0.05).结论 20 ng/kg生理剂量的CCK可舒张犬Oddi括约肌,大剂量CCK可兴奋犬Oddi括约肌;一氧化氮对犬Oddi括约肌发挥舒张效应,此效应可能在CCK的抑制通路中发挥重要作用.
目的 探討一氧化氮和膽囊收縮素(CCK)在犬Oddi括約肌運動調節中的作用.方法 測定正常狀態及註射CCK、硝普鈉、一氧化氮閤酶(NOS)抑製劑N-硝基-L-精氨痠甲酯(L-NAME)時,犬Oddi括約肌的基礎壓、時相收縮頻率、時相收縮幅度;免疫組織化學染色法檢測犬Oddi括約肌上NOS暘性神經元的錶達情況.計量資料的比較採用t檢驗.結果 註射0、20 ng/kg CCK後,犬Oddi括約肌基礎壓、時相收縮頻率、時相收縮幅度分彆為(27±10)mm Hg(1 mm Hg=0.133 kPa)、(10±3)次/min、(32±8)mm Hg和(61±14)mm Hg、(64±21)次/min、(44±15)mm Hg;註射100 ng/kg CCK後產生最大抑製作用和最大興奮作用時,犬Oddi括約肌基礎壓、時相收縮頻率、時相收縮幅度分彆為(77 +31)mm Hg、(69±18)次/min、(79±14)mm Hg和(140±21)mm Hg、(129±25)次/min、( 173±63)mm Hg.膽總管滴註硝普鈉後,犬Oddi括約肌基礎壓、時相收縮幅度均下降(t =3.706,5.183,P＜0.05).而註射L-NAME後,犬Oddi括約肌基礎壓、時相收縮幅度均升高(t=5.859,3.588,P＜0.05).結論 20 ng/kg生理劑量的CCK可舒張犬Oddi括約肌,大劑量CCK可興奮犬Oddi括約肌;一氧化氮對犬Oddi括約肌髮揮舒張效應,此效應可能在CCK的抑製通路中髮揮重要作用.
목적 탐토일양화담화담낭수축소(CCK)재견Oddi괄약기운동조절중적작용.방법 측정정상상태급주사CCK、초보납、일양화담합매(NOS)억제제N-초기-L-정안산갑지(L-NAME)시,견Oddi괄약기적기출압、시상수축빈솔、시상수축폭도;면역조직화학염색법검측견Oddi괄약기상NOS양성신경원적표체정황.계량자료적비교채용t검험.결과 주사0、20 ng/kg CCK후,견Oddi괄약기기출압、시상수축빈솔、시상수축폭도분별위(27±10)mm Hg(1 mm Hg=0.133 kPa)、(10±3)차/min、(32±8)mm Hg화(61±14)mm Hg、(64±21)차/min、(44±15)mm Hg;주사100 ng/kg CCK후산생최대억제작용화최대흥강작용시,견Oddi괄약기기출압、시상수축빈솔、시상수축폭도분별위(77 +31)mm Hg、(69±18)차/min、(79±14)mm Hg화(140±21)mm Hg、(129±25)차/min、( 173±63)mm Hg.담총관적주초보납후,견Oddi괄약기기출압、시상수축폭도균하강(t =3.706,5.183,P＜0.05).이주사L-NAME후,견Oddi괄약기기출압、시상수축폭도균승고(t=5.859,3.588,P＜0.05).결론 20 ng/kg생리제량적CCK가서장견Oddi괄약기,대제량CCK가흥강견Oddi괄약기;일양화담대견Oddi괄약기발휘서장효응,차효응가능재CCK적억제통로중발휘중요작용.
Objective To investigate the effects of nitric oxide (NO) and cholecystokinin (CCK) on the regulation of the motility of sphincter of Oddi (SO).Methods The basal pressure,action rate and contraction range of the SO were examined before and after the injection of CCK,sodium nitroprusside (SNP) and NG-nitroL-argininemethyl ester hydrochloride (L-NAME).The expression of neurons with positive expression of NO synthase was detected by immunohistochemical staining.The measurement data were analyzed by using the t test.Results The basal pressure,contraction rate and contraction range of the SO were (27 + 10)mm Hg (1 mm Hg =0.133 kPa),( 10 ± 3 ) times/minute and (32 + 8 ) mm Hg before injection of CCK,and (61 + 14) mm Hg,(64 +21 ) times/minute,(44 ± 15 ) mm Hg after injection of CCK of 20 ng/kg.After injection of CCK of 100 ng/kg,the basal pressure,contraction range and contraction rate of the SO were (77 ± 31 )mm Hg,(69 ± 18 ) times/minutes,(79 + 14) mm Hg when the inhibition effect of CCK reached peak,and were ( 140 ± 21 ) mm Hg,( 129 ± 25 )times/minutes,( 173 ± 63 ) mm Hg when the excitatory effect of CCK reached peak.After injection of SNP into the common bile duct,the basal pressure,contraction range and contraction rate of the SO decreased significantly ( t =3.706,5.183,P ＜ 0.05),while the 3 indexes increased significantly after injection of SNP (t =5.859,3.588,P ＜O.05).Conclusion Different from physiological dose (20 ng/kg) of CCK,large dose of CCK enhances motility of SO intensively.NO relaxes SO,which may play an important role in the inhibitor pathway of CCK.
