中华生物医学工程杂志
中華生物醫學工程雜誌
중화생물의학공정잡지
CHINESE JOURNAL OF BIOMEDICAL ENGINEERING
2009年
6期
431-435
,共5页
张新华%董颀%张志光%张同韩%陈广盛
張新華%董頎%張誌光%張同韓%陳廣盛
장신화%동기%장지광%장동한%진엄성
舌癌%顺铂%阿霉素%生长抑制%细胞周期
舌癌%順鉑%阿黴素%生長抑製%細胞週期
설암%순박%아매소%생장억제%세포주기
Tongue carcinoma%Cisplatin%Adriamycin%Growth inhibition%Cell cycle
目的 观察顺铂和阿霉素联合作用对人舌鳞癌Tca8113细胞生长的抑制作用,以及对细胞周期和凋亡的影响.方法 不同浓度顺铂、阿霉素及两者联合作用于人舌鳞癌Tca8113细胞,用MTT法观察细胞增殖情况以及药物对细胞生长的抑制效应.用流式细胞仪观察细胞凋亡和细胞周期.结果 顺铂(r=0.538,P<0.01)和阿霉素(r=0.642,P<0.01)呈剂量和时间依赖性抑制Tca8113细胞生长.单独用1.25 μmol/L阿霉素作用24 h,对Tca8113细胞的生长无显著影响.不同浓度顺铂(2～10mg/L)单独作用24 h,仅10 mg/L浓度可显著抑制Tca8113细胞的生长(P<0.01).1.25 μmol/L阿霉素与顺铂联合应用24h后,4、6、8、10mg/L顺铂均可显著抑制Tca8113细胞生长,且与相应浓度的单独用药组比,差异均有统计学意义(均为P<0.001).1.25 μmol/L阿霉素、6 mg/L顺铂以及阿霉素1.25 μmol/L+顺铂6 mg/L均显著改变G_0～G_1期(50.00±0.32%,43.53±2.026%.50.77±3.83%)和s期(36.5±1.05%.40.8±2.52%,30.87±2.65%)细胞百分比(均为P<0.01),联合用药的作用较单一用药组更显著(P<0.05).上述药物对细胞凋亡的作用均不明显,联合用药后坏死细胞比例明显增加.结论 顺铂和阿霉素联合作用,可显著地抑制人舌鳞癌细胞分化增殖,并增强药物的细胞毒性作用.
目的 觀察順鉑和阿黴素聯閤作用對人舌鱗癌Tca8113細胞生長的抑製作用,以及對細胞週期和凋亡的影響.方法 不同濃度順鉑、阿黴素及兩者聯閤作用于人舌鱗癌Tca8113細胞,用MTT法觀察細胞增殖情況以及藥物對細胞生長的抑製效應.用流式細胞儀觀察細胞凋亡和細胞週期.結果 順鉑(r=0.538,P<0.01)和阿黴素(r=0.642,P<0.01)呈劑量和時間依賴性抑製Tca8113細胞生長.單獨用1.25 μmol/L阿黴素作用24 h,對Tca8113細胞的生長無顯著影響.不同濃度順鉑(2～10mg/L)單獨作用24 h,僅10 mg/L濃度可顯著抑製Tca8113細胞的生長(P<0.01).1.25 μmol/L阿黴素與順鉑聯閤應用24h後,4、6、8、10mg/L順鉑均可顯著抑製Tca8113細胞生長,且與相應濃度的單獨用藥組比,差異均有統計學意義(均為P<0.001).1.25 μmol/L阿黴素、6 mg/L順鉑以及阿黴素1.25 μmol/L+順鉑6 mg/L均顯著改變G_0～G_1期(50.00±0.32%,43.53±2.026%.50.77±3.83%)和s期(36.5±1.05%.40.8±2.52%,30.87±2.65%)細胞百分比(均為P<0.01),聯閤用藥的作用較單一用藥組更顯著(P<0.05).上述藥物對細胞凋亡的作用均不明顯,聯閤用藥後壞死細胞比例明顯增加.結論 順鉑和阿黴素聯閤作用,可顯著地抑製人舌鱗癌細胞分化增殖,併增彊藥物的細胞毒性作用.
목적 관찰순박화아매소연합작용대인설린암Tca8113세포생장적억제작용,이급대세포주기화조망적영향.방법 불동농도순박、아매소급량자연합작용우인설린암Tca8113세포,용MTT법관찰세포증식정황이급약물대세포생장적억제효응.용류식세포의관찰세포조망화세포주기.결과 순박(r=0.538,P<0.01)화아매소(r=0.642,P<0.01)정제량화시간의뢰성억제Tca8113세포생장.단독용1.25 μmol/L아매소작용24 h,대Tca8113세포적생장무현저영향.불동농도순박(2～10mg/L)단독작용24 h,부10 mg/L농도가현저억제Tca8113세포적생장(P<0.01).1.25 μmol/L아매소여순박연합응용24h후,4、6、8、10mg/L순박균가현저억제Tca8113세포생장,차여상응농도적단독용약조비,차이균유통계학의의(균위P<0.001).1.25 μmol/L아매소、6 mg/L순박이급아매소1.25 μmol/L+순박6 mg/L균현저개변G_0～G_1기(50.00±0.32%,43.53±2.026%.50.77±3.83%)화s기(36.5±1.05%.40.8±2.52%,30.87±2.65%)세포백분비(균위P<0.01),연합용약적작용교단일용약조경현저(P<0.05).상술약물대세포조망적작용균불명현,연합용약후배사세포비례명현증가.결론 순박화아매소연합작용,가현저지억제인설린암세포분화증식,병증강약물적세포독성작용.
Objective To study the anti-tumor effect of cisplatin and adriamycin combination on the growth, cell cycle and apoptosis of tongue squamous cancer cell line Tca8113.Methods Different concentrations of cisplatin and/or adriamycin were added to Tca8113 cells.Cells proliferation was detected by MTT assay, and cell cycle and apoptosis detected by flow cytometry.Results Cisplatin(r=0.538, P<0.01) and adriamycin (r=0.642, P<0.01)inhibited cell growth in a dose- and time-dependent manner.Addition of 1.25 μmol/L adriamycin alone for 24 hours did not show any inhibition on the growth of Tea8113.With cisplatin (2～10 mg/L) alone for 24 hours, only 10 mg/L cisplatin inhibited the growth of TcaSll3 cells significantly (P<0.01).Combined with 1.25 μmol/L adriamycin, 24-hour treatment of cisplastin inhibited the cell growth at the levels of 4, 6, 8 and 10 mg/L which was significantly different as compared to the same level of cisplatin alone (all P<0.001).1.25 μmol/L adriamycin, 6 mg/L cisplatin and adriamycin 1.25 μmol/L + cisplatin 6 mg/L were all shown to significantly change G_0-G_1 phase (50.00±0.32%, 43.53±2.026%, 50.77± 3.83%) and S phase cell percentage (36.5±1.05%,40.8±2.52%, 30.87±2.65%) (all P<0.01), but the effect was more evident with combination of cisplatin and adriarnycin (P<0.05).While no obvious effect on apoptosis was found with these drags, combined use led to higher percentage of necrotizing cells.Conclusion Cisplatin and adriamycin combination can markedly inhibit the celia line Tca8113 differentiation and proliferation, and enhance cytotoxic effect of these two drugs.
