CAJ | 학술논문

目的了解经历不同类型的应激后大鼠抑郁样行为的差异.方法将新生的SD大鼠随机分为母爱剥夺应激组(27只)、慢性温和应激组(29只)、双重应激组(31只)和正常对照组(30只).母爱剥夺组大鼠于出生后即接受母爱剥夺应激,慢性温和应激组大鼠在成年后接受慢性温和应激,双重应激组大鼠先接受母爱剥夺应激,成年后再接受慢性温和应激,对照组不接受任何实验处理.采用强迫游泳实验和糖水偏爱实验作为评估大鼠抑郁样行为的指标.结果在强迫游泳实验中,母爱剥夺组[( 119.30±65.56)s]、慢性温和应激组[(145.00±80.24)s]及双重应激组[(170.03±61.75)s]大鼠静止漂浮时间长于对照组[(81.14±52.40)s,F=11.53,P＜0.01],其中双重应激组长于母爱剥夺应激组(P＜0.01),慢性应激组和双重应激组、母爱剥夺组大鼠差异无统计学意义(均P＞0.01)；在糖水偏爱实验中,慢性温和应激组[(0.43±0.28)]、双重应激组[(0.50±0.26)]和对照组[ (0.54±0.28)]大鼠的糖水偏爱率差异有统计学意义(F=4.33,P＞0.01),母爱剥夺组[(0.32±0.22)]的糖水偏爱率低于对照组和双重应激组(均P＜0.05)；雌、雄大鼠的静止漂浮时间和糖水偏爱率在不同应激方式下的差异无统计学意义(均P＞0.05).结论 3种应激方式均可导致大鼠表现出某种抑郁样行为.不同应激方式所致的抑郁样行为在不同性别的大鼠间无差异.
목적 료해경력불동류형적응격후대서억욱양행위적차이.방법 장신생적SD대서수궤분위모애박탈응격조(27지)、만성온화응격조(29지)、쌍중응격조(31지)화정상대조조(30지).모애박탈조대서우출생후즉접수모애박탈응격,만성온화응격조대서재성년후접수만성온화응격,쌍중응격조대서선접수모애박탈응격,성년후재접수만성온화응격,대조조불접수임하실험처리.채용강박유영실험화당수편애실험작위평고대서억욱양행위적지표.결과 재강박유영실험중,모애박탈조[( 119.30±65.56)s]、만성온화응격조[(145.00±80.24)s]급쌍중응격조[(170.03±61.75)s]대서정지표부시간장우대조조[(81.14±52.40)s,F=11.53,P＜0.01],기중쌍중응격조장우모애박탈응격조(P＜0.01),만성응격조화쌍중응격조、모애박탈조대서차이무통계학의의(균P＞0.01)；재당수편애실험중,만성온화응격조[(0.43±0.28)]、쌍중응격조[(0.50±0.26)]화대조조[ (0.54±0.28)]대서적당수편애솔차이유통계학의의(F=4.33,P＞0.01),모애박탈조[(0.32±0.22)]적당수편애솔저우대조조화쌍중응격조(균P＜0.05)；자、웅대서적정지표부시간화당수편애솔재불동응격방식하적차이무통계학의의(균P＞0.05).결론 3충응격방식균가도치대서표현출모충억욱양행위.불동응격방식소치적억욱양행위재불동성별적대서간무차이.
Objective In order to study the difference of depression-like behaviours among three widelyused stress models in rats.Methods The new-born Spragne-Dawley rats were randomly divided into maternal deprivation(MD) group(n=27),chronic mild stress(CMS) group (n=29),dual stress(DS) group ( n=31 ) and control (C) group ( n =30) on postnatal day 1.MD rats only received maternal deprivation.CMS rats only received chronic mild stress when 10 weeks old.DS rats received both maternal deprivation and chronic mild stress.Control rats received no experimental handling.Behavior tests including forced swimming test and sucrose consumption which were carried out to evaluate rats' depression-like behaviors in the thirteenth weeks.The extend time of floating and sucrose preference ratio should be recorded in the forced swimming test and sucrose consumption test respectively to reflect the behavior of helplessness and anhedonia of rats.Results In the forced swimming test,the extend floating time of MD group( 119.30 ± 65.56) s,CMS group ( 145.00 ± 80.24) s and DS group ( 170.03 ±61.75 )s were longer than the control group(81.14 ± 52.40)s (F =11.53,P ＜ 0.01 ).In the sucrose consumption test,the MD group(0.32 ± 0.22) had a low sucrose consumption.The comparison of the MD group and CMS group (0.43 ± 0.28 ) to the control group (0.54 ± 0.28 ) had significant differences (F =4.33,P ＜ 0.01 ).In these four groups,no sexual difference was found in the forced swimming test and sucrose consumption test ( all P ＞ 0.05 ).Conclusion The results suggest that MD,CMS and DS may induce some kind of depression-like behaviors in rodents such as anhedonia or the behavior of helplessness and the depression-like behaviors induced by different stresses are similar in male and female individuals.