中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2012年
7期
1221-1223
,共3页
刘振辉%易成腊%曹园%白祥军%李占飞%宋先舟
劉振輝%易成臘%曹園%白祥軍%李佔飛%宋先舟
류진휘%역성석%조완%백상군%리점비%송선주
脊髓损伤%醛糖还原酶%细胞凋亡
脊髓損傷%醛糖還原酶%細胞凋亡
척수손상%철당환원매%세포조망
Spinal cord injury%Aldose reductase%Cell apoptosis
目的 观察醛糖还原酶(AR)在急性脊髓损伤中的表达和醛糖还原酶抑制剂( ARI)对脊髓细胞凋亡的影响.方法 成年SD大鼠150只,随机分为3组:假手术对照组(Sham组,n=50只),脊髓损伤组(SCI组,n=50只),醛糖还原酶酶抑制剂(依帕司他片,唐林)组(ARI组,n=50只),每组大鼠分别分为3、12、24、48、72h5个时间点.假手术组只做椎板切除术,SCI组和ARI组采用Allen's法制备大鼠脊髓冲击伤模型.苏木素-伊红(HE)染色观察脊髓组织病理变化,双抗体夹心法检测AR含量,原位末端转移酶标记(TUNEL)法检测脊髓组织凋亡.结果 ARI组伤后3、12、24、48、72h损伤l内灰、白质结构破坏程度,空腔形成程度和炎性细胞浸润轻于同时间点的SCI组,假手术组未见到灰质和白质的破坏.Sham组3、12、24、48、72 h脊髓组织中AR含量分别为(10.75±0.47)、(10.61±0.44)、(10.62±0.55)、(10.92±0.49)、(10.57±0.52) U/L,SCI组对应时间点AR含量分别为(12.17±0.65、(14.09±0.61)、(14.58±0.72)、(15.45±0.47)、(15.33±0.29) U/L,ARI组对应时间点AR含量分别为(l0.84±0.21)、(12.70±0.47)、(12.84±0.55)、(12.28±0.54)、(11.95±0.90) U/L,SCI组AR含量在各个时间点均明显高于Sham组和ARI组(P＜0.05),Sham组各时间点很少见到脊髓凋亡细胞,SCI组3、12、24、48、72 h脊髓细胞凋亡率(％)分别为8.45±0.51、27.38±3.19、32.59±3.36、39.04±4.51、23.48±2.40,ARI组对应时间点脊髓细胞凋亡率(％)分别为8.21±0.52、18.69±1.63、22.64±2.18、25.61±3.53、16.48±2.23,SCI组脊髓神经元凋亡率在12、24、48、72 h高于ARI组(P＜0.05).结论 脊髓损伤后AR的表达增高,ARI能降低脊髓神经元细胞的凋亡率.
目的 觀察醛糖還原酶(AR)在急性脊髓損傷中的錶達和醛糖還原酶抑製劑( ARI)對脊髓細胞凋亡的影響.方法 成年SD大鼠150隻,隨機分為3組:假手術對照組(Sham組,n=50隻),脊髓損傷組(SCI組,n=50隻),醛糖還原酶酶抑製劑(依帕司他片,唐林)組(ARI組,n=50隻),每組大鼠分彆分為3、12、24、48、72h5箇時間點.假手術組隻做椎闆切除術,SCI組和ARI組採用Allen's法製備大鼠脊髓遲擊傷模型.囌木素-伊紅(HE)染色觀察脊髓組織病理變化,雙抗體夾心法檢測AR含量,原位末耑轉移酶標記(TUNEL)法檢測脊髓組織凋亡.結果 ARI組傷後3、12、24、48、72h損傷l內灰、白質結構破壞程度,空腔形成程度和炎性細胞浸潤輕于同時間點的SCI組,假手術組未見到灰質和白質的破壞.Sham組3、12、24、48、72 h脊髓組織中AR含量分彆為(10.75±0.47)、(10.61±0.44)、(10.62±0.55)、(10.92±0.49)、(10.57±0.52) U/L,SCI組對應時間點AR含量分彆為(12.17±0.65、(14.09±0.61)、(14.58±0.72)、(15.45±0.47)、(15.33±0.29) U/L,ARI組對應時間點AR含量分彆為(l0.84±0.21)、(12.70±0.47)、(12.84±0.55)、(12.28±0.54)、(11.95±0.90) U/L,SCI組AR含量在各箇時間點均明顯高于Sham組和ARI組(P＜0.05),Sham組各時間點很少見到脊髓凋亡細胞,SCI組3、12、24、48、72 h脊髓細胞凋亡率(％)分彆為8.45±0.51、27.38±3.19、32.59±3.36、39.04±4.51、23.48±2.40,ARI組對應時間點脊髓細胞凋亡率(％)分彆為8.21±0.52、18.69±1.63、22.64±2.18、25.61±3.53、16.48±2.23,SCI組脊髓神經元凋亡率在12、24、48、72 h高于ARI組(P＜0.05).結論 脊髓損傷後AR的錶達增高,ARI能降低脊髓神經元細胞的凋亡率.
목적 관찰철당환원매(AR)재급성척수손상중적표체화철당환원매억제제( ARI)대척수세포조망적영향.방법 성년SD대서150지,수궤분위3조:가수술대조조(Sham조,n=50지),척수손상조(SCI조,n=50지),철당환원매매억제제(의파사타편,당림)조(ARI조,n=50지),매조대서분별분위3、12、24、48、72h5개시간점.가수술조지주추판절제술,SCI조화ARI조채용Allen's법제비대서척수충격상모형.소목소-이홍(HE)염색관찰척수조직병리변화,쌍항체협심법검측AR함량,원위말단전이매표기(TUNEL)법검측척수조직조망.결과 ARI조상후3、12、24、48、72h손상l내회、백질결구파배정도,공강형성정도화염성세포침윤경우동시간점적SCI조,가수술조미견도회질화백질적파배.Sham조3、12、24、48、72 h척수조직중AR함량분별위(10.75±0.47)、(10.61±0.44)、(10.62±0.55)、(10.92±0.49)、(10.57±0.52) U/L,SCI조대응시간점AR함량분별위(12.17±0.65、(14.09±0.61)、(14.58±0.72)、(15.45±0.47)、(15.33±0.29) U/L,ARI조대응시간점AR함량분별위(l0.84±0.21)、(12.70±0.47)、(12.84±0.55)、(12.28±0.54)、(11.95±0.90) U/L,SCI조AR함량재각개시간점균명현고우Sham조화ARI조(P＜0.05),Sham조각시간점흔소견도척수조망세포,SCI조3、12、24、48、72 h척수세포조망솔(％)분별위8.45±0.51、27.38±3.19、32.59±3.36、39.04±4.51、23.48±2.40,ARI조대응시간점척수세포조망솔(％)분별위8.21±0.52、18.69±1.63、22.64±2.18、25.61±3.53、16.48±2.23,SCI조척수신경원조망솔재12、24、48、72 h고우ARI조(P＜0.05).결론 척수손상후AR적표체증고,ARI능강저척수신경원세포적조망솔.
Objective To discuss the expression of Aldose reductase in acute spinal cord injury and the effect of Aldose reductase inhibitor on spinal cell apoptosis.Methods One hundred and fifty SD adult rats were randomly assigned to sham group (n =50),acute spinal cord injury (n =50 ),Aldose reductase inhibitor (Epalrestat Tablets) group (n =50),each group is divided into five sub- groups(6,12,24,48,72 h sub-groups).In the sham group,only laminectomy was applied; In the SCI group and the ARI group,the model were made by Allen' s method.The pathological changes in injured spinal cord were observed with hematoxylin and eosin staining.The aldose reductase was detected by Elisa methods.The TdTmediated dUTP nick end labeling (TUNEL) method was employed to determine neuronal apoptosis.Results After injury 6,12,24,48 and 72 h,the grey and white matter structure of damage area were damaged,with cavity forming and inflammatory cells infiltrating.The degree of ARI group was lighted than SCI group.The content of aldose reductase in the sham operation group after injury 3,12,24,48,72 h were (10.75 ±0.47),(10.61 ±0.44),(10.62±0.55),(10.92±0.49),(10.57±0.52) U/L,the AR contents of SCI group at corresponding time were ( 12.17 ± 0.65 ),( 14.09 ± 0.61 ),( 14.58 ± 0.72 ),( 15.45 ±0.47 ),( 15.33 ± 0.29 ) U/L,and the AR content of the ARI group at corresponding time were ( 10.84 ±0.21),(12.70±0.47),(12.84±0.55),(12.28±0.54),(11.95±0.90) U/L.The AR content of the SCI group at each time point were significantly higher than the shame operation group and ARI group ( P ＜0.05 ).The spinal cord apoptotic cells were rarely seen in the sham operation group at each time point.The apoptotic rates (％) of SCI group after injuried 3,12,24,48,72 h were were 8.45±0.51,27.38 ± 3.19,32.59 ±3.36,39.04 ±4.51,23.48 ±2.40,and the rates of the ARI group at corresponding time were 8.21 ±0.52,18.69 ±1.63,22.64 ±2.18,25.61 ±3.53,16.48 ± 2.23.The neuron apoptosis rates of SCI group at 12,24 、48and 72 h were higher than that of the ARI group (P ＜ 0.05 ).Conclusion After acute spinal cord injury the expression of the AR increased,ARI may reduce the spinal cord neurons cell apoptosis.
