CAJ | 학술논문

目的观察间断皮下注射信号选择性甲状旁腺素(PTH)模拟肽对大鼠脊柱融合的影响.方法 32只雄性SD大鼠行后外侧路脊柱融合术,术后即用PTH模拟肽(Gly1,Arg19)人甲状旁腺素(hPTH)(1～28)[“G1,R19(1 ～28)”]、hPTH( 1-34)和(Gly1,Arg19)hPTH(1～ 34)[“G1,R19(1～34)”],经手法触诊、X线、融合节段骨矿含量(BMC)和骨密度(BMD)测定、显微CT(Micro-CT)和组织学综合评价各模拟肽对脊柱融合区骨组织生长的影响.结果手法触诊G1,R19(1 ～28)组、hPTH(1～ 34)组、G1,R19(1 ～ 34)组和空白对照组融合率分别为37.5％、50.0％、87.5％和50.0％；X线评分在G1,R19(1 ～28)组、hPTH(1～ 34)组、G1,R19(1 ～34)组和对照组分别为(1.88±1.36)、(2.58±1.32)、(3.88±0.75)和(2.54±1.32)分；融合节段BMC证实G1,R19(1～34)组明显高于其他3组(P＜0.01)；Micro-CT分析证实融合区骨体积在G1,R19(1 ～28)组、hPTH(1～34)组、G1,R19(1～34)组和对照组分别为(294.358±49.481)、(343.340±106.744)、(401.010±97.120)和(339.790±44.200) mm3.结论间断皮下注射G1,R19(1～34)能够增加大鼠后外侧路脊柱融合率及融合区的体积,且比hPTH(1～34)的效果更为显著.
목적 관찰간단피하주사신호선택성갑상방선소(PTH)모의태대대서척주융합적영향.방법 32지웅성SD대서행후외측로척주융합술,술후즉용PTH모의태(Gly1,Arg19)인갑상방선소(hPTH)(1～28)[“G1,R19(1 ～28)”]、hPTH( 1-34)화(Gly1,Arg19)hPTH(1～ 34)[“G1,R19(1～34)”],경수법촉진、X선、융합절단골광함량(BMC)화골밀도(BMD)측정、현미CT(Micro-CT)화조직학종합평개각모의태대척주융합구골조직생장적영향.결과 수법촉진G1,R19(1 ～28)조、hPTH(1～ 34)조、G1,R19(1 ～ 34)조화공백대조조융합솔분별위37.5％、50.0％、87.5％화50.0％；X선평분재G1,R19(1 ～28)조、hPTH(1～ 34)조、G1,R19(1 ～34)조화대조조분별위(1.88±1.36)、(2.58±1.32)、(3.88±0.75)화(2.54±1.32)분；융합절단BMC증실G1,R19(1～34)조명현고우기타3조(P＜0.01)；Micro-CT분석증실융합구골체적재G1,R19(1 ～28)조、hPTH(1～34)조、G1,R19(1～34)조화대조조분별위(294.358±49.481)、(343.340±106.744)、(401.010±97.120)화(339.790±44.200) mm3.결론 간단피하주사G1,R19(1～34)능구증가대서후외측로척주융합솔급융합구적체적,차비hPTH(1～34)적효과경위현저.
Objective To assess the effect of intermittent subcutaneous injection of ( Gly1,Arg19 )human parathyroid hormone (hPTH) (1-28 [“G1,R19 ( 1-28 )”],hPTH ( 1-34 ) and ( Gly1,Arg19 ) hPTH (1-34) [“G1,R19 (1 -34 )”] on posterolateral lumbar fusions in a rat model.Methods Thirty-two Sprague-Dawley rats were subjected to posterolateral intertransverse fusion with iliac bone autograft at L4-L5.After surgery,all Sprague-Dawley rats were randomized into 4 groups (n =8 each).Rats received G1,R19 (1-28) (200 μg/kg),hPTH(1-34) (15 μg/kg),G1,R19(1-34) (30 μg/kg) or saline (control) via infrascapular region subcutaneously at the third day after surgery (once every day,5 days per week).All rats were sacrificed 6 weeks after surgey by cervical dislocation.The L4-L5 segments were removed,and analyzed with manual palpation,X-ray,bone mineral density (BMD) and bone mineral content (BMC) in the fusion segments,Micro-CT and histology.Results At 6th week,manual palpation revealted that the fusion rate was 37.5％,50.0％,87.5％ and 50.0％ in G1,R19(1-28) group,hPTH(1-34) group,G1,R19 ( 1-34 ) group and control group respectively.By radiographic scoring system,the scores in G1,R19(1 -28) group hPTH( 1-34 ) group,G1,R19 ( 1-34 ) group and control group were ( 1.88 ± 1.36),(2.58 ±1.32),(3.88 ±0.75 ) and ( 2.54 ±1.32) respectively at 6th week after operation.BMD of the fusion segments in G1,R1g(1-34) group was higher than in other groups.BMC of the fusion segments in G1,R19 (1-34) group was higher than in other group (P ＜ 0.01).By micro-CT,fusion bone volume in G1,R19(1 -28) group,hPTH (1-34) group,G1,R19(1-34) group and control group was quantified as (294.358 ±49.481),(343.340 ± 106.744),(401.010 ±97.120) and (339.790 ±44.200) mm3 respectively.Conclusion Intermittent subcutaneous injection of [“G1,R19 (1-34)”] can raise the rate and area of posterolateral lumbar fusions in the rat model,and the effect is more notable than that of hPTH(1-34).