清华大学学报(英文版)
清華大學學報(英文版)
청화대학학보(영문판)
TSINGHUA SCIENCE AND TECHNOLOGY
2004年
1期
81-87
,共7页
周畅%温哲钘%王志萍%郭行%史冬梅%左焕琮%谢佐平
週暢%溫哲钘%王誌萍%郭行%史鼕梅%左煥琮%謝佐平
주창%온철형%왕지평%곽행%사동매%좌환종%사좌평
neural stem cell%electroporation transfection%enhanced green fluorescent protein%transplantation%migrate
Neural stem cells, which are clonogenic cells with self-renewal and multilineage differentiation properties, are currently considered as powerful candidates for cell replacement therapy in neurodegenerative disorders such as Parkinson's disease. A key issue is whether stem cells can survive, migrate and differentiate following transplantation into the adult central nervous system. This research shows that enhanced green fluorescent protein (EGFP) plasmid electroporation transfected neural stem cells can functionally differentiate in vitro and that most of the EGFP-positive cells can survive and migrate towards the damaged areas when transplanted into the brain of a Parkinson's disease model rat. The results suggest an effective and maneuverable tracing tool to detect whether transplanted neural stem and progenitor cells function in the adult brain in vivo.
