中华流行病学杂志
中華流行病學雜誌
중화류행병학잡지
CHINESE JOURNAL OF EPIDEMIOLOGY
2010年
7期
786-790
,共5页
高源%张砺%李马超%黄成%任红宇%周海健%朱兵清%李倩%王晓蕾%邵祝军
高源%張礪%李馬超%黃成%任紅宇%週海健%硃兵清%李倩%王曉蕾%邵祝軍
고원%장려%리마초%황성%임홍우%주해건%주병청%리천%왕효뢰%소축군
肺炎克雷伯菌%脉冲场凝胶电泳%多位点序列分型
肺炎剋雷伯菌%脈遲場凝膠電泳%多位點序列分型
폐염극뢰백균%맥충장응효전영%다위점서렬분형
Klebsiella pneumonia%Pulse-field gel electrophoresis%Multilocus sequence typing
目的 联合使用脉冲场凝胶电泳(PFGE)及多位点序列分型(MLST)对肺炎克雷伯菌进行分型研究.方法 参照美国疾病预防控制中心及亚太地区PulseNet提供的PFGE相关标准化操作程序,优化相关电泳条件,对某儿童医院于2005-2006年分离到的59株菌分型分析,对其中高度相似性菌株进行MLST分型分析.结果 59株菌经PFGE分型分析,可分成47个PFGE型,19个PFGE群,具有高度相似性的菌株经MLST分型,其型别分别为ST-340、ST-342、ST-343、ST-344、ST-345,其中ST-342、ST-343、ST-344、ST-345为MLST数据库中中国新发现的ST型.结论 具有PFGE高度相似性的肺炎克雷伯菌可以通过MLST进一步确认或区分.
目的 聯閤使用脈遲場凝膠電泳(PFGE)及多位點序列分型(MLST)對肺炎剋雷伯菌進行分型研究.方法 參照美國疾病預防控製中心及亞太地區PulseNet提供的PFGE相關標準化操作程序,優化相關電泳條件,對某兒童醫院于2005-2006年分離到的59株菌分型分析,對其中高度相似性菌株進行MLST分型分析.結果 59株菌經PFGE分型分析,可分成47箇PFGE型,19箇PFGE群,具有高度相似性的菌株經MLST分型,其型彆分彆為ST-340、ST-342、ST-343、ST-344、ST-345,其中ST-342、ST-343、ST-344、ST-345為MLST數據庫中中國新髮現的ST型.結論 具有PFGE高度相似性的肺炎剋雷伯菌可以通過MLST進一步確認或區分.
목적 연합사용맥충장응효전영(PFGE)급다위점서렬분형(MLST)대폐염극뢰백균진행분형연구.방법 삼조미국질병예방공제중심급아태지구PulseNet제공적PFGE상관표준화조작정서,우화상관전영조건,대모인동의원우2005-2006년분리도적59주균분형분석,대기중고도상사성균주진행MLST분형분석.결과 59주균경PFGE분형분석,가분성47개PFGE형,19개PFGE군,구유고도상사성적균주경MLST분형,기형별분별위ST-340、ST-342、ST-343、ST-344、ST-345,기중ST-342、ST-343、ST-344、ST-345위MLST수거고중중국신발현적ST형.결론 구유PFGE고도상사성적폐염극뢰백균가이통과MLST진일보학인혹구분.
Objective To type Klebsiella pneumonia through methods including pulse-field gel electrophoresis (PFGE) in combination with multilocus sequence typing. Methods Four selected different Eps, referring to the Standard Operating Procedure of PulseNet China, were used. The single colony of Klebsiella pneumonia was quantified after enriched culture. Embedding organisms in agarose and genome DNA were lysed with Proteinase K and then digested by restriction endonuclease Xba Ⅰ , to produce agarose gel. Fingerprint was obtained through PFGE and bands were marked with their molecular weights and then analyzed by BioNumerics software. Using MLST to analyze the strains that were highly similar, by PFGE typing Results By comparing the four results from each Eps, fk3 (switch time from 6s to 36s,total run time is 18.5 hours) seemed to be better than the others.59 strains of Klebsiella pneumonia were divided into 47 PFGE types and 19 PFGE clusters. The highly similar strains could be typed into ST-340、ST-342、ST-343、ST-344、ST-345 by MLST. Among them, ST-342、 ST-343、 ST-344、 ST-345 types were all new MLST types that were reported in China.Conclusion Highly similar Klebsiella pneumonias typed by PFGE could also be typed by MLST.
