CAJ | 학술논문

目的探讨有机阴离子转运体1B1和1B3(编码基因分别为SLCO1B1和SLCO1B3)基因多态性对肾移植受者血麦考酚酸(MPA)浓度的影响.方法用聚合酶链反应-连接酶检测反应法对68例汉族肾移植受者的SLCO1B1和1B3基因进行单核苷酸基因多态性(SNP)检测,分别检测SLCO1B3 T334G和SLCO1B1 A338G的突变位点.术后第28天,收集受者血浆检测MPA浓度.比较各基因型受者的MPA浓度-时间曲线下面积(AUC0-12),分析单核苷酸多态性与MPA AUC0-12之间的相关性.结果 SLCO1B3 T334G位点GG基因型受者的MPA AUG0-12高于TT基因型受者,分别为(54.54±14.40)mg·h·L-1和(37.30±12.88)mg·h·L-1(P=0.052);SLCO1B1 A338G位点的各个基因型受者之间MPA AUC0-12比较,差异均无统计学意义(P>0.05).结论 SLCO1B3基因多态性对肾移植受者术后血MPA浓度的个体间差异产生明显影响.
목적 탐토유궤음리자전운체1B1화1B3(편마기인분별위SLCO1B1화SLCO1B3)기인다태성대신이식수자혈맥고분산(MPA)농도적영향.방법 용취합매련반응-련접매검측반응법대68례한족신이식수자적SLCO1B1화1B3기인진행단핵감산기인다태성(SNP)검측,분별검측SLCO1B3 T334G화SLCO1B1 A338G적돌변위점.술후제28천,수집수자혈장검측MPA농도.비교각기인형수자적MPA농도-시간곡선하면적(AUC0-12),분석단핵감산다태성여MPA AUC0-12지간적상관성.결과 SLCO1B3 T334G위점GG기인형수자적MPA AUG0-12고우TT기인형수자,분별위(54.54±14.40)mg·h·L-1화(37.30±12.88)mg·h·L-1(P=0.052);SLCO1B1 A338G위점적각개기인형수자지간MPA AUC0-12비교,차이균무통계학의의(P>0.05).결론 SLCO1B3기인다태성대신이식수자술후혈MPA농도적개체간차이산생명현영향.
Objective To analyze the relationship between the genetic polymorphisms of organic anion transporting polypeptide (SLCO1B1 and SLCO1B3) and mycophenolic acid ( MPA)pharmacokinetics in Chinese kidney transplant recipients. Methods Gene mutations (SLCO1B3T334G, SLCO1B1 A338G) were detected in 68 recipients by PCR-LDR. The plasma samples were collected and blood concentration of MPA was measured on the 28 th day after transplantation. The area under the curve (AUC)0-12 of MPA in different genotype recipients was compared to analyze the correlation between single nucleotide polymorphisms (SNPs) and MPA pharmacokinetics. Results MPA AUC0-12 was higher in SLCO1B3 T334G GG carriers group than in TT carriers [(54. 54 ±14.40)vs(37.30±12.88)mg·h·L-1,(P=0.052)].However,there was no difference in MPA AUC0-12 among each genotype of SLCO1B1 A338G (P>0. 05). Conclusion Genetic polymorphisms of SLCO1B3 affect interindividual variety in plasma MPA concentration in Chinese kidney transplantation recipients.