CAJ | 학술논문

目的构建原代人结直肠癌裸鼠盲肠原位移植瘤模型(肝转移模型),探讨Kiss-1基因在移植瘤存活、生长和转移中的作用.方法选择Kiss-1基因阳性和阴性表达的人结直肠癌组织块各1块,分别建立裸鼠皮下移植瘤和裸鼠盲肠原位移植瘤模型,并观察移植瘤生长和肝转移.结果裸鼠第2代皮下移植瘤模型构建成功率Kiss-1基因阳性组为37.50％,阴性组为100.00％,差异有统计学意义(P＜0.05)；裸鼠盲肠原位移植瘤模型构建成功率,第1代为41.67％(Kiss-1阳性组为12.25％,阴性组75.00％),第2代为75.00％(Kiss-1阳性组为56.25％,阴性组为93.75％)；Kiss-1阴性组成功率均高于阳性组,差异有统计学意义(P＜0.05)；皮下移植瘤模型和第1代盲肠原位移植瘤模型中均未发现肝转移,第2代盲肠原位移植瘤模型中肝转移率Kiss-1阳性组为33.33％,阴性组80.00％,差异有统计学意义(P＜0.05).结论 Kiss-1基因表达缺失有助于提高原代人结直肠癌裸鼠盲肠原位移植瘤肝转移模型构建的成功率,提示它具有抑制结直肠癌移植瘤存活、生长和转移的作用.
목적 구건원대인결직장암라서맹장원위이식류모형(간전이모형),탐토Kiss-1기인재이식류존활、생장화전이중적작용.방법 선택Kiss-1기인양성화음성표체적인결직장암조직괴각1괴,분별건립라서피하이식류화라서맹장원위이식류모형,병관찰이식류생장화간전이.결과 라서제2대피하이식류모형구건성공솔Kiss-1기인양성조위37.50％,음성조위100.00％,차이유통계학의의(P＜0.05)；라서맹장원위이식류모형구건성공솔,제1대위41.67％(Kiss-1양성조위12.25％,음성조75.00％),제2대위75.00％(Kiss-1양성조위56.25％,음성조위93.75％)；Kiss-1음성조성공솔균고우양성조,차이유통계학의의(P＜0.05)；피하이식류모형화제1대맹장원위이식류모형중균미발현간전이,제2대맹장원위이식류모형중간전이솔Kiss-1양성조위33.33％,음성조80.00％,차이유통계학의의(P＜0.05).결론 Kiss-1기인표체결실유조우제고원대인결직장암라서맹장원위이식류간전이모형구건적성공솔,제시타구유억제결직장암이식류존활、생장화전이적작용.
Objective To construct the nude mice orthotopic model (nude mice liver metastasis model) transplanted with colorectal carcinoma,and to investigate the roles of Kiss-1 gene on survival,growth and metastasis of transplanted tumors.Methods The colorectal carcinoma tissues positive or negative for Kiss-1 gene expression were chosen to construct the nude mice subcutaneous model and orthotopic transplanted model.The growth of tumor and the liver metastasis was observed.Results In the nude mice subcutaneous model,the success rate in the groups positive or negative for Kiss-1 was 37.50％ and 100.00％ with the difference being statistically significant ( P ＜ 0.05).In the orthotopic transplanted model,the success rate of the first generation was 41.67％ ( 12.25％ for positive Kiss-1,and 75.00％ for negative Kiss-1 ),and that of the second generation was 41.67％ (56.25％ for positive Kiss-1 and 93.75％ for negative Kiss-1 ).The success rate in Kiss-1 negative groups was higher than in Kiss-1 negative groups (P ＜ 0.05).In the second generation of the orthotopic transplanted model,the incidence of liver metastasis in Kiss-1 positive or negative groups was 33.33％ and 80.00％ respectively (P ＜ 0.05 ).Conclusion The loss of Kiss-1 expression might increase the success rate of the nude mice orthotopic model transplanted with colorectal carcinoma (nude mice liver metastasis model),suggesting Kiss-1 might restrain the survival,growth and metastasis of the transplanted colorectal carcinomas.