中华肿瘤杂志
中華腫瘤雜誌
중화종류잡지
CHINESE JOURNAL OF ONCOLOGY
2012年
1期
15-20
,共6页
倪勤%张勇晶%张善春%金明娟%马新源%姚开颜%李其龙%陈坤
倪勤%張勇晶%張善春%金明娟%馬新源%姚開顏%李其龍%陳坤
예근%장용정%장선춘%금명연%마신원%요개안%리기룡%진곤
结直肠肿瘤%基因,H-ras%基因,L-myc%多态性,单核苷酸
結直腸腫瘤%基因,H-ras%基因,L-myc%多態性,單覈苷痠
결직장종류%기인,H-ras%기인,L-myc%다태성,단핵감산
Colorectal neoplasms%Gene,H-ras%Gene,L-myc%Polymorphism,single nucleotide
目的 探讨原癌基因L-myc和H-ras的多态性以及基因-基因交互作用与结直肠癌发病风险的关系.方法 应用病例对照研究,采用聚合酶链反应-限制性片段长度多态性方法检测浙江省嘉善县373例结直肠癌患者和838例健康对照的L-myc、H-ras基因型.采用Mantel-Haenzsel分层分析、叉生分析等统计学方法分析基因-基因交互作用,并采用多因子降维(MDR)模型进行验证.结果 单基因模型显示,L-myc和H-ras的单个基因多态性与结直肠癌的发病风险无关(P＞0.05).分层分析提示,在L-myc LS+ SS基因型携带者中,H-ras TC+ CC基因型携带者相对于TT基因型携带者患直肠癌的风险显著增加(OR=1.81,P=0.005),但不增加结肠癌、结直肠癌的发病风险(均P＞0.05).Logistic回归分析显示,分层后H-ras基因多态性与结肠癌、直肠癌和结直肠癌发病均无关(均P ＞0.05).在携带LL基因型的人群中,以TT基因型为参照,TC+ CC基因型不增加结肠癌、直肠癌和结直肠癌的发病风险(均P ＞0.05).叉生分析结果显示,结肠癌、直肠癌和结直肠癌患者L-myc和H-ras基因的主效应均无统计学意义(均P＞0.05).交互作用分析结果显示,结肠癌和结直肠癌患者中L-myc和H-ras基因不存在交互作用,在直肠癌中存在正相乘模型的交互作用(OR=2.74,P=0.024).MDR模型验证结果显示,L-myc和H-ras在直肠癌中存在基因-基因交互作用,交叉验证一致率为100％ (P =0.001).结论 H-ras和L-myc多态性与结肠癌、直肠癌和结直肠癌发病风险无关,H-ras和L-myc之间的基因-基因交互作用增加直肠癌的发病风险.
目的 探討原癌基因L-myc和H-ras的多態性以及基因-基因交互作用與結直腸癌髮病風險的關繫.方法 應用病例對照研究,採用聚閤酶鏈反應-限製性片段長度多態性方法檢測浙江省嘉善縣373例結直腸癌患者和838例健康對照的L-myc、H-ras基因型.採用Mantel-Haenzsel分層分析、扠生分析等統計學方法分析基因-基因交互作用,併採用多因子降維(MDR)模型進行驗證.結果 單基因模型顯示,L-myc和H-ras的單箇基因多態性與結直腸癌的髮病風險無關(P＞0.05).分層分析提示,在L-myc LS+ SS基因型攜帶者中,H-ras TC+ CC基因型攜帶者相對于TT基因型攜帶者患直腸癌的風險顯著增加(OR=1.81,P=0.005),但不增加結腸癌、結直腸癌的髮病風險(均P＞0.05).Logistic迴歸分析顯示,分層後H-ras基因多態性與結腸癌、直腸癌和結直腸癌髮病均無關(均P ＞0.05).在攜帶LL基因型的人群中,以TT基因型為參照,TC+ CC基因型不增加結腸癌、直腸癌和結直腸癌的髮病風險(均P ＞0.05).扠生分析結果顯示,結腸癌、直腸癌和結直腸癌患者L-myc和H-ras基因的主效應均無統計學意義(均P＞0.05).交互作用分析結果顯示,結腸癌和結直腸癌患者中L-myc和H-ras基因不存在交互作用,在直腸癌中存在正相乘模型的交互作用(OR=2.74,P=0.024).MDR模型驗證結果顯示,L-myc和H-ras在直腸癌中存在基因-基因交互作用,交扠驗證一緻率為100％ (P =0.001).結論 H-ras和L-myc多態性與結腸癌、直腸癌和結直腸癌髮病風險無關,H-ras和L-myc之間的基因-基因交互作用增加直腸癌的髮病風險.
목적 탐토원암기인L-myc화H-ras적다태성이급기인-기인교호작용여결직장암발병풍험적관계.방법 응용병례대조연구,채용취합매련반응-한제성편단장도다태성방법검측절강성가선현373례결직장암환자화838례건강대조적L-myc、H-ras기인형.채용Mantel-Haenzsel분층분석、차생분석등통계학방법분석기인-기인교호작용,병채용다인자강유(MDR)모형진행험증.결과 단기인모형현시,L-myc화H-ras적단개기인다태성여결직장암적발병풍험무관(P＞0.05).분층분석제시,재L-myc LS+ SS기인형휴대자중,H-ras TC+ CC기인형휴대자상대우TT기인형휴대자환직장암적풍험현저증가(OR=1.81,P=0.005),단불증가결장암、결직장암적발병풍험(균P＞0.05).Logistic회귀분석현시,분층후H-ras기인다태성여결장암、직장암화결직장암발병균무관(균P ＞0.05).재휴대LL기인형적인군중,이TT기인형위삼조,TC+ CC기인형불증가결장암、직장암화결직장암적발병풍험(균P ＞0.05).차생분석결과현시,결장암、직장암화결직장암환자L-myc화H-ras기인적주효응균무통계학의의(균P＞0.05).교호작용분석결과현시,결장암화결직장암환자중L-myc화H-ras기인불존재교호작용,재직장암중존재정상승모형적교호작용(OR=2.74,P=0.024).MDR모형험증결과현시,L-myc화H-ras재직장암중존재기인-기인교호작용,교차험증일치솔위100％ (P =0.001).결론 H-ras화L-myc다태성여결장암、직장암화결직장암발병풍험무관,H-ras화L-myc지간적기인-기인교호작용증가직장암적발병풍험.
Objective To explore the association between the polymorphisms of oncogenes H-ras and L-myc and colorectal cancer risk,and the interaction of those genes.Methods The genotypes of H-ras and L-myc genes were determined by polymerase chain reaction-based restriction fragment length polymorphism analysis.Stratified analysis and logistic model were used to detect the gene-gene interaction.The gene-gene interaction was validated by multifactor dimensionality reduction ( MDR ) analysis. Results The single SNP model showed that the polymorphisms of H-ras and L-myc genes were not significantly related with colorectal cancer risk (P ＞ 0.05).Stratified analysis revealed that among the L-myc LS + SS genotype carriers,those with H-ras TC + CC genotype showed significantly increased risk of rectal cancer than those with TT genotype (OR =1.81,P =0.005).The positive interaction between L-myc and H-ras was detected by logistic regression model.The OR of the interaction effect was 2.74 (P =0.024 ).This result was confirmed in the MDR model,with 54.83％ testing balanced accuracy and 10/10 cross-validation consistency,and the model was still significant after the 1000 times permutation test ( P =0.001 ).Conclusion Our findings suggest that the polymorphism of H-ras and L-myc genes is not related to colorectal cancer risk,but there is a synergy between H-ras and L-myc polymorphisms in the development of rectal cancer.
