中华医学遗传学杂志
中華醫學遺傳學雜誌
중화의학유전학잡지
CHINESE JOURNAL OF MEDICAL GENETICS
2012年
1期
60-63
,共4页
王皖芬%王凤%朱敏%黄洁%周元林%林仙方%胡晓飞%李卫玲%张细六%金笑平
王皖芬%王鳳%硃敏%黃潔%週元林%林仙方%鬍曉飛%李衛玲%張細六%金笑平
왕환분%왕봉%주민%황길%주원림%림선방%호효비%리위령%장세륙%금소평
基质金属蛋白酶-8%遗传多态性%脑梗死%颈动脉疾病
基質金屬蛋白酶-8%遺傳多態性%腦梗死%頸動脈疾病
기질금속단백매-8%유전다태성%뇌경사%경동맥질병
Matrix metalloproteinase-8%Genetic polymorphism Infarction%Carotid artery disease
目的 探讨我国浙江地区汉族人群基质金属蛋白酶-8 (matrix metalloproteinase-8,MMP-8)启动子基因-799C/T多态与颈动脉斑块易损性的关系.方法 451例脑梗死患者根据颈动脉超声分为易损斑块组(共135例)和稳定斑块组(共316例).采用酶联免疫吸附法测定两组血清MMP-8水平,并采用聚合酶链反应-限制性片段长度多态性方法分析两组MMP-8启动子基因-799C/T多态的基因型.结果 易损斑块组发病48 h内血清MMP-8水平为(0.86±0.16)ng/μL,稳定斑块组为(0.80±0.13) ng/μL,两者差异有统计学意义(t=2.894,P=0.004).易损斑块组CT+ TT基因型频率为73.3％,稳定斑块组为54.7％,两者差异有统计学意义(x2=13.65,P=0.000)；T等位基因频率在易损斑块组为48.1％,在稳定斑块组为33.5％,两者差异有统计学意义(x2=17.14,P=0.000).CC基因型组血清MMP-8水平为(0.79±0.13) ng/μL,TT基因型组血清MMP-8水平为(0.92±0.11) ng/μL,两者比较差异有统计学意义(t=3.141,P=0.001).结论 MMP-8启动子基因-799C/T多态可能与浙江地区汉族人颈动脉易损斑块发生的倾向有关,T等位基因可能是颈动脉斑块易损的遗传易患性标志之一.
目的 探討我國浙江地區漢族人群基質金屬蛋白酶-8 (matrix metalloproteinase-8,MMP-8)啟動子基因-799C/T多態與頸動脈斑塊易損性的關繫.方法 451例腦梗死患者根據頸動脈超聲分為易損斑塊組(共135例)和穩定斑塊組(共316例).採用酶聯免疫吸附法測定兩組血清MMP-8水平,併採用聚閤酶鏈反應-限製性片段長度多態性方法分析兩組MMP-8啟動子基因-799C/T多態的基因型.結果 易損斑塊組髮病48 h內血清MMP-8水平為(0.86±0.16)ng/μL,穩定斑塊組為(0.80±0.13) ng/μL,兩者差異有統計學意義(t=2.894,P=0.004).易損斑塊組CT+ TT基因型頻率為73.3％,穩定斑塊組為54.7％,兩者差異有統計學意義(x2=13.65,P=0.000)；T等位基因頻率在易損斑塊組為48.1％,在穩定斑塊組為33.5％,兩者差異有統計學意義(x2=17.14,P=0.000).CC基因型組血清MMP-8水平為(0.79±0.13) ng/μL,TT基因型組血清MMP-8水平為(0.92±0.11) ng/μL,兩者比較差異有統計學意義(t=3.141,P=0.001).結論 MMP-8啟動子基因-799C/T多態可能與浙江地區漢族人頸動脈易損斑塊髮生的傾嚮有關,T等位基因可能是頸動脈斑塊易損的遺傳易患性標誌之一.
목적 탐토아국절강지구한족인군기질금속단백매-8 (matrix metalloproteinase-8,MMP-8)계동자기인-799C/T다태여경동맥반괴역손성적관계.방법 451례뇌경사환자근거경동맥초성분위역손반괴조(공135례)화은정반괴조(공316례).채용매련면역흡부법측정량조혈청MMP-8수평,병채용취합매련반응-한제성편단장도다태성방법분석량조MMP-8계동자기인-799C/T다태적기인형.결과 역손반괴조발병48 h내혈청MMP-8수평위(0.86±0.16)ng/μL,은정반괴조위(0.80±0.13) ng/μL,량자차이유통계학의의(t=2.894,P=0.004).역손반괴조CT+ TT기인형빈솔위73.3％,은정반괴조위54.7％,량자차이유통계학의의(x2=13.65,P=0.000)；T등위기인빈솔재역손반괴조위48.1％,재은정반괴조위33.5％,량자차이유통계학의의(x2=17.14,P=0.000).CC기인형조혈청MMP-8수평위(0.79±0.13) ng/μL,TT기인형조혈청MMP-8수평위(0.92±0.11) ng/μL,량자비교차이유통계학의의(t=3.141,P=0.001).결론 MMP-8계동자기인-799C/T다태가능여절강지구한족인경동맥역손반괴발생적경향유관,T등위기인가능시경동맥반괴역손적유전역환성표지지일.
Objective To investigate the association between a -799C/T polymorphism in the promotor region of matrix metalloproteinase-8 (MMP-8)gene and instability of carotid plaque in Chinese Han population.Methods A total of 451 acute infarction patients from the Department of Neurology of Taizhou Hospital were divided into carotid vulnerable plaque group and carotid stable plaque group according to the results of carotid B-mode ultrasonography. Serum MMP-8 level was measured by the means of enzyme-linked immunosorbent assay (ELISA).At the same time,the MMP-8 -799C/T polymorphism was determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method.Results Serum level of MMP-8 in the carotid vulnerable plaque group was higher than that in the carotid stable plaque group (t=2.894,P=0.004).The genotype distribution of -799C/T polymorphism between the two groups was significantly different (x2 =13.65,P=0.000).Serum level of MMP-8 in patients with TT genotype was higher than that in patients with CC genotype (t=3.141,P=0.001).Conclusion The present study suggested that serum level of MMP-8 and the - 799C/T polymorphism of MMP-8 gene are associated with carotid vulnerable plaque in Chinese Han population,and the T allele may be a predictor for the susceptibility of carotid vulnerable plaque.
