中华预防医学杂志
中華預防醫學雜誌
중화예방의학잡지
CHINESE JOURNAL OF
2011年
7期
597-600
,共4页
管振坡%谷连坤%邢宝才%季加孚%顾晋%邓大君
管振坡%穀連坤%邢寶纔%季加孚%顧晉%鄧大君
관진파%곡련곤%형보재%계가부%고진%산대군
肿瘤%基因表达%聚合酶链反应
腫瘤%基因錶達%聚閤酶鏈反應
종류%기인표체%취합매련반응
Neoplasms%Gene expression%Polymerase chain reaction
目的 研究色素框同源蛋白7(cbx7)基因在结肠癌、胃癌和肝癌组织中的表达变化,探讨这种表达变化与肿瘤发生和发展的相关性.方法 收集22例结肠癌患者、20例胃癌患者、30例肝癌患者的肿瘤组织及其切缘组织标本、临床病理资料和随访资料.提取各标本RNA,用荧光定量PCR检测cbx7 mRNA含量,分析肿瘤组织中cbx7 mRNA表达水平与患者临床特征相关性及与患者术后生存时间的关系.结果 结肠癌、胃癌和肝癌组织中cbx7 mRNA的相对拷贝数分别为0.010±0.015、0.197±0.195、0.008±0.008;对应各癌组织的切缘正常组织中cbx7 mRNA的相对拷贝数分别为0.053±0.042、1.891±1.254、0.030±0.021.与切缘正常组织相比,3种癌组织中的cbx7 mRNA含量均明显下调(结肠癌:t=-7.351,P<0.01;胃癌:t=-5.417,P<0.01;肝癌:t=-6.680,P<0.01).结肠癌患者中,年龄>55岁组cbx7 mRNA相对拷贝数为0.007±0.015,≤55岁组为0.017±0.012,两组间差异有统计学意义(t=-2.586,P=0.022);有脉管癌栓组cbx7 mRNA的相对拷贝数为0.022±0.021,无癌栓组为0.006±0.011,两组间差异有统计学意义(t=-3.175,P=0.010).通过受试者特征曲线[ROC曲线,曲线下面积为0.769(P=0.033)]确定结肠癌患者cbx7 mRNA相对拷贝数的Cut-off值为0.002,小于0.002者确定为表达降低;cbx7低表达的结肠癌患者术后生存时间短,5年生存率仅为30.8%(4/13),而cbx7高表达患者5年生存率达77.8%(7/9),差异有统计学意义(χ2=4.329,P=0.037).在胃癌和肝癌患者中未见此现象.结论 在结肠癌、胃癌、肝癌组织中cbx7的表达明显下降;cbx7 mRNA的表达含量变化影响结肠癌患者预后.
目的 研究色素框同源蛋白7(cbx7)基因在結腸癌、胃癌和肝癌組織中的錶達變化,探討這種錶達變化與腫瘤髮生和髮展的相關性.方法 收集22例結腸癌患者、20例胃癌患者、30例肝癌患者的腫瘤組織及其切緣組織標本、臨床病理資料和隨訪資料.提取各標本RNA,用熒光定量PCR檢測cbx7 mRNA含量,分析腫瘤組織中cbx7 mRNA錶達水平與患者臨床特徵相關性及與患者術後生存時間的關繫.結果 結腸癌、胃癌和肝癌組織中cbx7 mRNA的相對拷貝數分彆為0.010±0.015、0.197±0.195、0.008±0.008;對應各癌組織的切緣正常組織中cbx7 mRNA的相對拷貝數分彆為0.053±0.042、1.891±1.254、0.030±0.021.與切緣正常組織相比,3種癌組織中的cbx7 mRNA含量均明顯下調(結腸癌:t=-7.351,P<0.01;胃癌:t=-5.417,P<0.01;肝癌:t=-6.680,P<0.01).結腸癌患者中,年齡>55歲組cbx7 mRNA相對拷貝數為0.007±0.015,≤55歲組為0.017±0.012,兩組間差異有統計學意義(t=-2.586,P=0.022);有脈管癌栓組cbx7 mRNA的相對拷貝數為0.022±0.021,無癌栓組為0.006±0.011,兩組間差異有統計學意義(t=-3.175,P=0.010).通過受試者特徵麯線[ROC麯線,麯線下麵積為0.769(P=0.033)]確定結腸癌患者cbx7 mRNA相對拷貝數的Cut-off值為0.002,小于0.002者確定為錶達降低;cbx7低錶達的結腸癌患者術後生存時間短,5年生存率僅為30.8%(4/13),而cbx7高錶達患者5年生存率達77.8%(7/9),差異有統計學意義(χ2=4.329,P=0.037).在胃癌和肝癌患者中未見此現象.結論 在結腸癌、胃癌、肝癌組織中cbx7的錶達明顯下降;cbx7 mRNA的錶達含量變化影響結腸癌患者預後.
목적 연구색소광동원단백7(cbx7)기인재결장암、위암화간암조직중적표체변화,탐토저충표체변화여종류발생화발전적상관성.방법 수집22례결장암환자、20례위암환자、30례간암환자적종류조직급기절연조직표본、림상병리자료화수방자료.제취각표본RNA,용형광정량PCR검측cbx7 mRNA함량,분석종류조직중cbx7 mRNA표체수평여환자림상특정상관성급여환자술후생존시간적관계.결과 결장암、위암화간암조직중cbx7 mRNA적상대고패수분별위0.010±0.015、0.197±0.195、0.008±0.008;대응각암조직적절연정상조직중cbx7 mRNA적상대고패수분별위0.053±0.042、1.891±1.254、0.030±0.021.여절연정상조직상비,3충암조직중적cbx7 mRNA함량균명현하조(결장암:t=-7.351,P<0.01;위암:t=-5.417,P<0.01;간암:t=-6.680,P<0.01).결장암환자중,년령>55세조cbx7 mRNA상대고패수위0.007±0.015,≤55세조위0.017±0.012,량조간차이유통계학의의(t=-2.586,P=0.022);유맥관암전조cbx7 mRNA적상대고패수위0.022±0.021,무암전조위0.006±0.011,량조간차이유통계학의의(t=-3.175,P=0.010).통과수시자특정곡선[ROC곡선,곡선하면적위0.769(P=0.033)]학정결장암환자cbx7 mRNA상대고패수적Cut-off치위0.002,소우0.002자학정위표체강저;cbx7저표체적결장암환자술후생존시간단,5년생존솔부위30.8%(4/13),이cbx7고표체환자5년생존솔체77.8%(7/9),차이유통계학의의(χ2=4.329,P=0.037).재위암화간암환자중미견차현상.결론 재결장암、위암、간암조직중cbx7적표체명현하강;cbx7 mRNA적표체함량변화영향결장암환자예후.
Objective To investigate the relationship between chromobox protein homolog 7 (cbx7) expression and the occurrence and development of colorectal carcinoma (CRC),gastric carcinoma (GC) and hepatocarcinoma (HCC) tissues.Methods The samples of neoplastic tissues and the corresponding cutting-edge normal tissues from 22 cases of CRC,20 cases of GC,30 cases of HCC were surgically collected.Level of cbx7 mRNA was detected with a fluorescent quantitative RT-PCR assay,and the correlationship among expression of cbx7 mRNA,the patients′ clinicopathologic features and the surviving time after surgery was analyzed.Results The relative copy number of cbx7 mRNA in carcinomas and the normal tissues was 0.010±0.015 vs 0.053±0.042 for CRCs,0.197±0.195 vs 1.891±1.254 for GCs,and 0.008±0.008 vs 0.030±0.021 for HCCs,respectively.Compared with the corresponding normal tissues,cbx7 expression was significantly downregulated in CRCs,GCs,and HCCs (t=-7.351,-5.417 and -6.680,respectively,P<0.01).The expression of cbx7 mRNA in CRCs had significant differences not only between two age groups (the relative copy number of cbx7 mRNA in age>55 group was 0.007±0.015,but 0.017±0.012 in age≤55 group,t=-2.586 ,P=0.022); but also between vascular embolus-positive and negative groups (the level of cbx7 mRNA in positive and negative group was 0.022±0.021 vs 0.006±0.011,t=-3.175,P=0.010).The area under the receiver operating characteristics(ROC) curve is 0.769 (P=0.033).when the Cut-off value of the relative copy number of cbx7 mRNA was 0.002 in CRCs.The values less-than 0.002 were defined as low expression.The CRC patients with low expression of cbx7 had a shorter overall survival time; whose 5 years survival rate was only 30.8%(4/13); while the rate was 77.8% (7/9) in high expression of cbx7 group.The difference had statistical significance (χ2=4.329,P=0.037).The similar differences could not be found among GC and HCC patients.Conclusion Downregulation of cbx7 expression was very common among multiple carcinomas cases,and the downregulation influenced the prognosis of CRC patients.
