CAJ | 학술논문

通过体外抑制肿瘤细胞生长活性筛选确定青天葵95%乙醇(体积分数)提取物及其石油醚萃取部位为抗肿瘤活性部位.综合利用多种色谱手段及重结晶方法从石油醚部位分离得到了8个化合物,并运用理化性质和波谱技术分析确定了化合物结构.其结构分别为:环桉烯醇(cycloeucalenol)(1);豆甾醇(2);谷甾醇(3);熊果酸(4);aurantiamide acetate(5);(20S,22E,24R)-ergosta-7,22-dien-3β,5a,6β-triol(6);6-methoxy-cerevisteml(7);和β-胡萝卜苷(8).其中,化合物3、5、6、7和8均为首次从该植物中分离得到.
통과체외억제종류세포생장활성사선학정청천규95%을순(체적분수)제취물급기석유미췌취부위위항종류활성부위.종합이용다충색보수단급중결정방법종석유미부위분리득도료8개화합물,병운용이화성질화파보기술분석학정료화합물결구.기결구분별위:배안희순(cycloeucalenol)(1);두치순(2);곡치순(3);웅과산(4);aurantiamide acetate(5);(20S,22E,24R)-ergosta-7,22-dien-3β,5a,6β-triol(6);6-methoxy-cerevisteml(7);화β-호라복감(8).기중,화합물3、5、6、7화8균위수차종해식물중분리득도.
The results of the in vitro antitumor activity screenings indicated that 95% ethanol extract and the petroleum ether fraction from the extract showed a significant inhibitory activities on growth of cancer cells. Eight compounds were isolated from this fraction by a combination of various chromatogra-phy methods and re-crystallization. The chemical structures were elucidated on the basis of physico-chem-ical properties and spectral data, and were identified as cycloeucalenol (1) ; stigmaterol (2) ; sitosterol (3) ; ursolic acid (4) ; aurantiamide acetate (5) ; (20S, 22E, 24R)-ergosta-7,22-dien-3β,5α,6β-triol (6) ; 6-methoxy-cerevisterol (7) ; andβ-daucosterol (8). Among these compounds, 3, 5, 6, 7 and 8 are obtained from the plant for the first time.