四川大学学报（医学版）
四川大學學報（醫學版）
사천대학학보（의학판）
JOURNAL OF SICHUAN UNIVERSITY(MEDICAL SCIENCE EDITION)
2010年
2期
296-298,311
,共4页
肖登%杨霖%雷中杰%杨永红%高强%何成奇
肖登%楊霖%雷中傑%楊永紅%高彊%何成奇
초등%양림%뢰중걸%양영홍%고강%하성기
脉冲电磁场%去卵巢大鼠%生物力学
脈遲電磁場%去卵巢大鼠%生物力學
맥충전자장%거란소대서%생물역학
Pulsed electromagnetic fields%Ovariectomized rat%Biomechanical property
目的 通过采用不同治疗时间脉冲电磁场(PEMFs)干预去卵巢大鼠骨质疏松模型,观察各组大鼠的股骨生物力学性能的变化,以探索PEMFs治疗骨质疏松的最适治疗时间.方法 将雌性3月龄SD大鼠50只随机分为5组:SHAM组、OVX_0组、OVX_Ⅰ组、OVX_Ⅱ组、OVX_Ⅲ组,每组10只.各OVX组均按文献方法切除双侧卵巢造模,SHAM组仅开腹不切除卵巢.OVX_Ⅰ、OVX_Ⅱ和OVX_Ⅲ 3组大鼠每天在强度为3.8 mT,频率为8 Hz的磁场环境中分别照射20、40、60 min,共30 d.SHAM组和OVX_0组不干预.各组动物均在实验完成后股动脉放血处死,取右侧股骨作生物力学性能测定.结果 OVX_0组大鼠的股骨结构力学指标(包括最大位移、最大载荷及最大能量吸收,下同) 和材料力学指标(包括最大应力、最大应变及弹性模量,下同)低于其它4组大鼠(P<0.05或P<0.01),但SHAM组、OVX_Ⅰ组、OVX_Ⅱ组及OVX_Ⅲ组4组大鼠的股骨生物力学指标之间的差异无统计学意义(P>0.05).结论 采用磁场强度(3.8 mT)和脉冲频率(8 Hz)PEMFs,每天治疗20～60 min,可阻止去卵巢大鼠的股骨生物力学性能下降,使其接近正常的水平,其骨生物力学性能的维持效果相同.
目的 通過採用不同治療時間脈遲電磁場(PEMFs)榦預去卵巢大鼠骨質疏鬆模型,觀察各組大鼠的股骨生物力學性能的變化,以探索PEMFs治療骨質疏鬆的最適治療時間.方法 將雌性3月齡SD大鼠50隻隨機分為5組:SHAM組、OVX_0組、OVX_Ⅰ組、OVX_Ⅱ組、OVX_Ⅲ組,每組10隻.各OVX組均按文獻方法切除雙側卵巢造模,SHAM組僅開腹不切除卵巢.OVX_Ⅰ、OVX_Ⅱ和OVX_Ⅲ 3組大鼠每天在彊度為3.8 mT,頻率為8 Hz的磁場環境中分彆照射20、40、60 min,共30 d.SHAM組和OVX_0組不榦預.各組動物均在實驗完成後股動脈放血處死,取右側股骨作生物力學性能測定.結果 OVX_0組大鼠的股骨結構力學指標(包括最大位移、最大載荷及最大能量吸收,下同) 和材料力學指標(包括最大應力、最大應變及彈性模量,下同)低于其它4組大鼠(P<0.05或P<0.01),但SHAM組、OVX_Ⅰ組、OVX_Ⅱ組及OVX_Ⅲ組4組大鼠的股骨生物力學指標之間的差異無統計學意義(P>0.05).結論 採用磁場彊度(3.8 mT)和脈遲頻率(8 Hz)PEMFs,每天治療20～60 min,可阻止去卵巢大鼠的股骨生物力學性能下降,使其接近正常的水平,其骨生物力學性能的維持效果相同.
목적 통과채용불동치료시간맥충전자장(PEMFs)간예거란소대서골질소송모형,관찰각조대서적고골생물역학성능적변화,이탐색PEMFs치료골질소송적최괄치료시간.방법 장자성3월령SD대서50지수궤분위5조:SHAM조、OVX_0조、OVX_Ⅰ조、OVX_Ⅱ조、OVX_Ⅲ조,매조10지.각OVX조균안문헌방법절제쌍측란소조모,SHAM조부개복불절제란소.OVX_Ⅰ、OVX_Ⅱ화OVX_Ⅲ 3조대서매천재강도위3.8 mT,빈솔위8 Hz적자장배경중분별조사20、40、60 min,공30 d.SHAM조화OVX_0조불간예.각조동물균재실험완성후고동맥방혈처사,취우측고골작생물역학성능측정.결과 OVX_0조대서적고골결구역학지표(포괄최대위이、최대재하급최대능량흡수,하동) 화재료역학지표(포괄최대응력、최대응변급탄성모량,하동)저우기타4조대서(P<0.05혹P<0.01),단SHAM조、OVX_Ⅰ조、OVX_Ⅱ조급OVX_Ⅲ조4조대서적고골생물역학지표지간적차이무통계학의의(P>0.05).결론 채용자장강도(3.8 mT)화맥충빈솔(8 Hz)PEMFs,매천치료20～60 min,가조지거란소대서적고골생물역학성능하강,사기접근정상적수평,기골생물역학성능적유지효과상동.
Objective To test the effect of pulsed electromagnetic fields (PEMFs) with different length of treatment on the biomechanical properties of femurs in ovariectomized rats.Methods Fifty female SD rats were randomly divided into five groups: ① SHAM control (no PEMFs treatment), ② OVX_0 control (no PEMFs treatment), ③ OVX_Ⅰ (PEMFs treatment at 8 Hz frequency with 3.8 mT intensity, 20 min daily for 30 days), ④ OVX_Ⅱ (PEMFs treatment at 8 Hz frequency with 3.8 mT intensity, 40 min daily for 30 days), and ⑤ OVX_Ⅲ(PEMFs treatment at 8 Hz frequency with 3.8 mT intensity, 60 min daily for 30 days).All of the rats were subject to bilateral overiectomy except those in the SHAM control group.The biomechanical properties of the femurs were assessed at the end of the PEMFs treatment.Results The rats in the OVX_0 control group had significantly lower values in the biomechanical properties than the rats in the other four groups (P<0.05 or P<0.01).The rats treated with PEMFs showed no significant differences in the biomechanical properties compared with the sham controls (P>0.05).Conclusion PEMFs therapy at 8 Hz and 3.8 mT magnetic intensity for 20 to 60 min everyday prevents decline in biomechanical properties of femurs in ovariectomized rat.
