CAJ | 학술논문

目的:建立天胡荽类药材HPLC指纹图谱的鉴别方法.方法:以Diamonsil C_(18)(250 mm×4.6 mm,5 μm)色谱柱为分析柱,流动相为乙腈0.1%磷酸水,采用梯度洗脱,流速1.0 mL/min,340 nm波长下检测.结果:天胡荽类药材的HPLC指纹图谱有明显差异.结论:该方法简便,重复性好,特征性强,可用于天胡荽类药材的鉴别.
목적:건립천호유류약재HPLC지문도보적감별방법.방법:이Diamonsil C_(18)(250 mm×4.6 mm,5 μm)색보주위분석주,류동상위을정0.1%린산수,채용제도세탈,류속1.0 mL/min,340 nm파장하검측.결과:천호유류약재적HPLC지문도보유명현차이.결론:해방법간편,중복성호,특정성강,가용우천호유류약재적감별.
AIM: To establish HPLC fingerprint for the identification of Hydrocotyle sibthorpioides and its adulterants. METHODS: The chromatographic seperation was performed on a Diamonsil C_(18) (250 mm×4.6 mm,5 μm)with a mobile phase consisting of acetonitrile-0.1% phosphoric acid, gradient eluent, at the flow rate of 1.0 mL/min. The UV detection was set at 340 nm. RESULTS: There was an apparent difference in fingerprint between Hydrocotyle sibthorpioides and its adulterants. CONCLUSION: The method is stable and reliable with a good reproducibility and provides a reference standard for identifying Hydrocotyle sibthorpioides and its adulterants.