中国医师进修杂志
中國醫師進脩雜誌
중국의사진수잡지
CHINESE JOURNAL OF POSTGRADUATES OF MEDICINE
2012年
19期
30-32
,共3页
糖尿病肾病%凝血致活酶%糖尿病,2型
糖尿病腎病%凝血緻活酶%糖尿病,2型
당뇨병신병%응혈치활매%당뇨병,2형
Diabetic nephropathies%Thromboplastin%Diabetes mellitus,type 2
目的 检测2型糖尿病肾病(DN)患者血液和尿液中组织因子(TF)表达水平和促凝血活性,探讨其相关的临床意义.方法 收集120例DN患者,按尿白蛋白与肌酐比值分为正常白蛋白尿组(＜3.4 mg/mmol)64例、微量白蛋白尿组(≥3.4 mg/mmol而＜34.1 mg/mmol)35例、大量白蛋白尿组(≥34.1 mg/mmol)21例.另选取健康查体者20例作为健康对照组.流式细胞仪检测外周血单核细胞TF的表达率,ELISA法检测血液和尿液中TF的表达水平,一步凝固法检测TF的促凝血活性.结果 正常白蛋白尿组、微量白蛋白尿组和大量白蛋白尿组单核细胞TF的表达率分别为(4.32±1.25)％、(5.01±1.73)％和(11.83±2.14)％,TF促凝血活性分别为(64.3±21.2)、(67.1±17.3)和( 113.8±44.4) mU/L,明显高于健康对照组的(0.84±0.36)％、(23.8±16.4) mU/L,差异有统计学意义(P＜0.01):正常白蛋白尿组和微量白蛋白尿组比较差异无统计学意义(P＞0.05),但大量白蛋白尿组与正常白蛋白尿组和微量白蛋白尿组比较差异有统计学意义(P＜0.01).DN患者血液和尿液中TF表达水平明显高于健康对照组(P＜0.01),血液中的TF表达水平与尿液中的TF表达水平呈正相关(r=0.684,P＜0.01).结论 DN患者血液和尿液中TF表达水平增加且促凝血活性增强,其表达水平的高低与蛋白尿的增加相关.
目的 檢測2型糖尿病腎病(DN)患者血液和尿液中組織因子(TF)錶達水平和促凝血活性,探討其相關的臨床意義.方法 收集120例DN患者,按尿白蛋白與肌酐比值分為正常白蛋白尿組(＜3.4 mg/mmol)64例、微量白蛋白尿組(≥3.4 mg/mmol而＜34.1 mg/mmol)35例、大量白蛋白尿組(≥34.1 mg/mmol)21例.另選取健康查體者20例作為健康對照組.流式細胞儀檢測外週血單覈細胞TF的錶達率,ELISA法檢測血液和尿液中TF的錶達水平,一步凝固法檢測TF的促凝血活性.結果 正常白蛋白尿組、微量白蛋白尿組和大量白蛋白尿組單覈細胞TF的錶達率分彆為(4.32±1.25)％、(5.01±1.73)％和(11.83±2.14)％,TF促凝血活性分彆為(64.3±21.2)、(67.1±17.3)和( 113.8±44.4) mU/L,明顯高于健康對照組的(0.84±0.36)％、(23.8±16.4) mU/L,差異有統計學意義(P＜0.01):正常白蛋白尿組和微量白蛋白尿組比較差異無統計學意義(P＞0.05),但大量白蛋白尿組與正常白蛋白尿組和微量白蛋白尿組比較差異有統計學意義(P＜0.01).DN患者血液和尿液中TF錶達水平明顯高于健康對照組(P＜0.01),血液中的TF錶達水平與尿液中的TF錶達水平呈正相關(r=0.684,P＜0.01).結論 DN患者血液和尿液中TF錶達水平增加且促凝血活性增彊,其錶達水平的高低與蛋白尿的增加相關.
목적 검측2형당뇨병신병(DN)환자혈액화뇨액중조직인자(TF)표체수평화촉응혈활성,탐토기상관적림상의의.방법 수집120례DN환자,안뇨백단백여기항비치분위정상백단백뇨조(＜3.4 mg/mmol)64례、미량백단백뇨조(≥3.4 mg/mmol이＜34.1 mg/mmol)35례、대량백단백뇨조(≥34.1 mg/mmol)21례.령선취건강사체자20례작위건강대조조.류식세포의검측외주혈단핵세포TF적표체솔,ELISA법검측혈액화뇨액중TF적표체수평,일보응고법검측TF적촉응혈활성.결과 정상백단백뇨조、미량백단백뇨조화대량백단백뇨조단핵세포TF적표체솔분별위(4.32±1.25)％、(5.01±1.73)％화(11.83±2.14)％,TF촉응혈활성분별위(64.3±21.2)、(67.1±17.3)화( 113.8±44.4) mU/L,명현고우건강대조조적(0.84±0.36)％、(23.8±16.4) mU/L,차이유통계학의의(P＜0.01):정상백단백뇨조화미량백단백뇨조비교차이무통계학의의(P＞0.05),단대량백단백뇨조여정상백단백뇨조화미량백단백뇨조비교차이유통계학의의(P＜0.01).DN환자혈액화뇨액중TF표체수평명현고우건강대조조(P＜0.01),혈액중적TF표체수평여뇨액중적TF표체수평정정상관(r=0.684,P＜0.01).결론 DN환자혈액화뇨액중TF표체수평증가차촉응혈활성증강,기표체수평적고저여단백뇨적증가상관.
Objective To detect the expression level and procoagulant activity of tissue factor (TF)in the blood and urine in patients with type 2 diabetic nephropathy (DN),and investigate its related clinical significance.Methods A total of 120 DN patients were collected and divided into 3 groups according to urinary albumin and creatinine ratio:normal albuminuria group (＜3.4 mg/mmol) with 64 cases,microalbuminuria group ( ≥3.4 mg/nunol,and ＜ 34.1 mg/mmol) with 35 cases and macroalbuminuria group (≥34.1 mg/mnol) with 21 cases.Twenty healthy persons were enrolled as control group.The expression of monocytes TF in peripheral blood was detected by flow cytometric analyzer.The expression ofTF in the blood and urine was detected by ELLSA.The prcoagulant activity of TF was detected by one-stage coagulation.Results The expressions of monocytes TF in normal albuminuria group,microalbuminuria group and macroalbuminuria group [ (4.32 ± 1.25 )％,(5.01 ± 1.73)％,( 1 1.83 ± 2.14)％ ] were significantly higher than that in control group [(0.84 ± 0.36)％ ](P ＜0.01).TF procoagulant activity in normal albuminuria group,microalbuminuria group and macroalbuminuria group [ ( 64.3 ± 21.2),( 67.1 ± 17.3 ),( 113.8 ± 44.4)mU/L] were obviously higher than that in control grouP [ (23.8 ± 16.4) mU/L](P＜ 0.01 ).The expression of monocytes TF in normal albuminuria group and microalbuminuria group had no statistical significance (P＞0.05),while the expression in macroalbuminuria group had significant difference compared with that in normal albuminuria group and microalbuminuria group (P ＜ 0.01 ).The expression of TF in the blood and urine of DN patients was obviously higher than that of control group (P ＜ 0.01 ).The expression of TF in the blood was positively correlated with that in urine (r =0.684,P ＜ 0.01 ).Conclusions The expressions of TF in the blood and urine in DN patients increase and its procoagulant activity enhances.The expression level of TF has a closed relationship with the increase of albuminuria.
