CAJ | 학술논문

目的探讨维生素D受体(VDR)对肠道肿瘤生长的影响以及与β-catenin信号通路之间的关系.方法体外培养的人类结肠癌SW480细胞株经维生素D处理4 h,免疫沉淀法检测VDR和β-catenin蛋白的交互作用,24 h后用Western blot检测细胞E-cadherin蛋白的表达.体内实验比较APCmin/+VDR-/-与APCmin/+小鼠,免疫组化法检测两型小鼠肠道肿瘤VDR、β-catenin和BrdU蛋白的表达,Western blot检测肿瘤和肿瘤旁组织β-catenin蛋白的表达.结果维生素D处理SW480细胞后,VDR和β-catenin蛋白相互结合,随后E-cadherin蛋白表达增高(对照组灰度值145.57±4.21,实验组109.35±3.56,t＝32.63,P＜0.05).缺失VDR的APCmin/+VDR-/-小鼠肠道肿瘤中β-catenin蛋白表达免疫组化(灰度值140.51±2.57)及Western blot(灰度值166.47±2.36)检测均高于APCmin/+肿瘤(分别为145.41±3.62、182.35±3.24,t＝2.65,4.36,P＜0.05).结论维生素D抑制肠道肿瘤增殖的作用可能通过VDR影响β-catenin信号通路来完成.
목적 탐토유생소D수체(VDR)대장도종류생장적영향이급여β-catenin신호통로지간적관계.방법 체외배양적인류결장암SW480세포주경유생소D처리4 h,면역침정법검측VDR화β-catenin단백적교호작용,24 h후용Western blot검측세포E-cadherin단백적표체.체내실험비교APCmin/+VDR-/-여APCmin/+소서,면역조화법검측량형소서장도종류VDR、β-catenin화BrdU단백적표체,Western blot검측종류화종류방조직β-catenin단백적표체.결과 유생소D처리SW480세포후,VDR화β-catenin단백상호결합,수후E-cadherin단백표체증고(대조조회도치145.57±4.21,실험조109.35±3.56,t＝32.63,P＜0.05).결실VDR적APCmin/+VDR-/-소서장도종류중β-catenin단백표체면역조화(회도치140.51±2.57)급Western blot(회도치166.47±2.36)검측균고우APCmin/+종류(분별위145.41±3.62、182.35±3.24,t＝2.65,4.36,P＜0.05).결론 유생소D억제장도종류증식적작용가능통과VDR영향β-catenin신호통로래완성.
Objective To explore the effect of vitamin D receptor (VDR) in intestinal tumor development and the relationship between VDR and β-catenin signaling pathway. Methods The interaction of vitamin D receptor and β-catenin were detected by co-immunoprecipitation assay after human colonic carcinoma cells SW480 were treated with vitamin D in vitro for 4 hours. The expression of E-cadherin protein was detected by Western blot after treated for 24 hours. To compare APCmin/+VDR-/- and APCmin/+ mice in vivo, the expression of VDR,β-catenin and BrdU proteins in intestinal tumor were determined by immunohistochemistry. The expression of β-catenin protein in tumor and adjacency intestinal was further determined by Western blot. Results After SW480 cells were treated with vitamin D, vitamin D receptor and β-catenin protein showed binding, the expression of E-cadherin protein further increased (Gray value the control group 145.57±4.21,Gray value of the experimental group 109.35±3.56,t＝32.63,P＜0.05). Immunostaining and Western blot detection（Gray value 166.47±2.36） showed a marked increase of β-catenin level(Gray value 140.51±2.57) in APCmin/+VDR-/- tumor compared to APCmin/+ tumor(145.41±3.62,182.35±3.24,t＝2.65,4.36,P＜0.05). Conclusions The role of vitamin D suppressing intestinal tumor may be achieved through VDR affectingβ-catenin signaling pathway.