CAJ | 학술논문

目的比较氨基葡萄糖对体外培养软骨和滑膜细胞软骨寡聚基质蛋白(COMP)分泌的影响.方法软骨细胞和滑膜细胞采自骨关节炎(OA)患者,采用分阶段酶消化法体外培养人软骨细胞和滑膜细胞,给予实验兔以氨基葡萄糖临床等效剂量灌胃后,抽取含药血清培养细胞.采用酶联免疫吸附试验(ELISA)检测药物对体外培养的人软骨细胞和滑膜细胞培养上清液中COMP的浓度.结果体外培养滑膜细胞分泌的COMP浓度[(41.6±0.4)ng/ml]显著高于软骨细胞[(5.5±3.0)ng/ml](19＜0.05).氨基葡萄糖可显著增加体外培养软骨细胞的COMP分泌[(20.3±3.6)与(5.5±3.0)ng/ml,P＜0.05];而对体外培养滑膜细胞的作用较弱,可轻度减少COMP分泌[(36.6±1.3)与(41.6±0.4)ng/ml].结论氨基葡萄糖含药血清可以促进体外培养软骨细胞分泌COMP,而对体外培养滑膜细胞无明显作用.
목적 비교안기포도당대체외배양연골화활막세포연골과취기질단백(COMP)분비적영향.방법 연골세포화활막세포채자골관절염(OA)환자,채용분계단매소화법체외배양인연골세포화활막세포,급여실험토이안기포도당림상등효제량관위후,추취함약혈청배양세포.채용매련면역흡부시험(ELISA)검측약물대체외배양적인연골세포화활막세포배양상청액중COMP적농도.결과 체외배양활막세포분비적COMP농도[(41.6±0.4)ng/ml]현저고우연골세포[(5.5±3.0)ng/ml](19＜0.05).안기포도당가현저증가체외배양연골세포적COMP분비[(20.3±3.6)여(5.5±3.0)ng/ml,P＜0.05];이대체외배양활막세포적작용교약,가경도감소COMP분비[(36.6±1.3)여(41.6±0.4)ng/ml].결론 안기포도당함약혈청가이촉진체외배양연골세포분비COMP,이대체외배양활막세포무명현작용.
Objective To compare the effect of glucosamine (Virtral-s) on the cartilage oligomeric matrix protein (COMP) secretion of chondrocytes and synoviocytes in vitro.Methods Chondrocytes and synoviocytes isolated from knee cartilage of osteoarthritic patients were cultured by phased enzymatic digestion.Sera containing Virtral-s of the experimental animals were obtained after orally administrated Virtral-s at the dosages that equal to human.Cells were cultured in the medium with Virtral-s containing sera.Super-natant COMP level was tested by enzyme-linked immunoabsorbent assays (ELISA).Results COMP conceu-tration of synoviocytes cultured in vitro was significantly higher than that of chondrocytes (P＜0.05).Virtral-s could significantly increase COMP secretion in cultured chondrocytes in vitro (P＜0.05),however,it had a weaker role on synoviocytes,ie,it could only mildly reduce COMP secretion of synoviocytes.Conclusion Glucosamine (Virtral-s)-containing serum can promote COMP secretion of chondrocytes in vitro,and it has no significant effect on synoviocytes in vitro.