中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2012年
8期
582-583
,共2页
目的 构建疣状毛癣菌感染豚鼠的动物模型.方法 选择健康豚鼠32只,随机分为4组.第1组未给任何处置,第2、4组豚鼠刮毛并摩擦其背部皮肤,第3组豚鼠背部给予刮毛处理.第1、2、3组为实验观察组,分别涂抹疣状毛癣菌菌悬液100 μl;第4组涂抹须毛癣菌菌悬液100μl.采用直接镜检、真菌培养和组织病理方法验证感染结果.结果 第1组直接镜检持续阴性；第2、3、4组豚鼠背部鳞屑直接镜检至第7～9天时均为阳性；将鳞屑接种于沙氏葡萄糖琼脂(SDA)平板上培养,7d后可见真菌生长,2周后各平板接种点均有菌落生长,且与接种前菌落形态基本相同.第2、3、4组取皮损活检HE染色,表皮角质层及部分毛囊漏斗部可见折光性强的菌丝及孢子.结论 成功构建了疣状毛癣菌感染的豚鼠模型.
目的 構建疣狀毛癬菌感染豚鼠的動物模型.方法 選擇健康豚鼠32隻,隨機分為4組.第1組未給任何處置,第2、4組豚鼠颳毛併摩抆其揹部皮膚,第3組豚鼠揹部給予颳毛處理.第1、2、3組為實驗觀察組,分彆塗抹疣狀毛癬菌菌懸液100 μl;第4組塗抹鬚毛癬菌菌懸液100μl.採用直接鏡檢、真菌培養和組織病理方法驗證感染結果.結果 第1組直接鏡檢持續陰性；第2、3、4組豚鼠揹部鱗屑直接鏡檢至第7～9天時均為暘性；將鱗屑接種于沙氏葡萄糖瓊脂(SDA)平闆上培養,7d後可見真菌生長,2週後各平闆接種點均有菌落生長,且與接種前菌落形態基本相同.第2、3、4組取皮損活檢HE染色,錶皮角質層及部分毛囊漏鬥部可見摺光性彊的菌絲及孢子.結論 成功構建瞭疣狀毛癬菌感染的豚鼠模型.
목적 구건우상모선균감염돈서적동물모형.방법 선택건강돈서32지,수궤분위4조.제1조미급임하처치,제2、4조돈서괄모병마찰기배부피부,제3조돈서배부급여괄모처리.제1、2、3조위실험관찰조,분별도말우상모선균균현액100 μl;제4조도말수모선균균현액100μl.채용직접경검、진균배양화조직병리방법험증감염결과.결과 제1조직접경검지속음성；제2、3、4조돈서배부린설직접경검지제7～9천시균위양성；장린설접충우사씨포도당경지(SDA)평판상배양,7d후가견진균생장,2주후각평판접충점균유균락생장,차여접충전균락형태기본상동.제2、3、4조취피손활검HE염색,표피각질층급부분모낭루두부가견절광성강적균사급포자.결론 성공구건료우상모선균감염적돈서모형.
Objective To establish a guinea pig model of dermatophytosis caused by Trichophyton verrucosum.Methods Thirty-two guinea pigs were randomly and equally divided into four groups to remain untreated (group 1 ),receive depilation and skin abrasion in the back (group 2 and 4),or only depilation (group 3).The back was smeared with a suspension (100 μl) of Trichophyton verrucosum in group 1,2 and 3,and with that of Trichophyton mentagrophytes in group 4.Direct microscopy,fungal culture and histopathology were performed to validate the infection of guinea pigs by Trichophyton.Results Direct microscopy was persistently negative in group 1,turned to be positive from the 7th to 9th day in group 2,3,and 4.Colonies began to appear 7 days after the inoculation of scrapings onto Sabouraud's dextrose agar (SDA) in group 2 and 3,and were observed at all the inoculated sites 2 weeks after the inoculation,with a morphological similarity to Trichophyton verrucosum before inoculation into guinea pigs.Hematoxylin and eosin staining revealed strongly refractive hyphae and spores in stratum corneum epidermidis and infundibular regions of some hair follicles in group 2 and 3.Conclusion A guinea pig model of dermatophytosis caused by Trichophyton verrucosum is successfully constructed.
