CAJ | 학술논문

目的探讨间充质干细胞体外构建组织工程血管的可行性.方法将体外培养扩增的犬骨髓间充质干细胞(MSCs)定向分化为平滑肌样细胞和内皮样细胞,接种于ε-己内酯/L-丙交酯(PCLA)支架上,将其置于生物反应器内,在搏动性力学(100±20/55±20)mm Hg(1 mm Hg=0.133 kPa)刺激条件下培养.3 d后行血管组织学检测.结果血管支架拉伸强度6.1 MPa;骨髓间充质干细胞成功定向分化为平滑肌样细胞和内皮样细胞;血管腔内表面完全为细胞覆盖,表面的细胞沿液体流动的方向分布;种植的部分细胞已经渗透入血管壁内.结论骨髓间充质干细胞可作为种子细胞,与PCLA支架在生物反应器内构建组织工程血管.
목적 탐토간충질간세포체외구건조직공정혈관적가행성.방법 장체외배양확증적견골수간충질간세포(MSCs)정향분화위평활기양세포화내피양세포,접충우ε-기내지/L-병교지(PCLA)지가상,장기치우생물반응기내,재박동성역학(100±20/55±20)mm Hg(1 mm Hg=0.133 kPa)자격조건하배양.3 d후행혈관조직학검측.결과 혈관지가랍신강도6.1 MPa;골수간충질간세포성공정향분화위평활기양세포화내피양세포;혈관강내표면완전위세포복개,표면적세포연액체류동적방향분포;충식적부분세포이경삼투입혈관벽내.결론 골수간충질간세포가작위충자세포,여PCLA지가재생물반응기내구건조직공정혈관.
Objective To investigate the feasibility of constructing small-caliber tissue engineered blood vessels in vitro. Methods Canine mesenchymal stem cells (MSCs) were cultured and differentiated to endothelial cells (ECs) and smooth muscular cells (SMCs) in vitro. The SMCs and ECs were seeded on the lumen of the PCLA scaffold. The scaffold was connected to a pulsatile flow chamber in bioreactor. After its culturing in a bioreactor with mechanical stimulation ( 100 ± 20/55 ± 20) mm Hg (1 mm Hg =0. 133 kPa)for 3 days, the scaffold was observed under the scanning electron microscopy and by using immunofluorescence staining. Results The tensile strength of scaffold was 6. 1 MPa. The MSCs were differentiated to ECs and SMCs. A confluent cellular monolayer covering the inner surface of the scaffold was found. Cells were elongated and aligned in the direction of the blood flow. The seeding cells were infiltrated into the interstitia of the PCLA scaffold. Conclusion MSCs as seeding cells and PCLA as scaffold are able to construct a tissue engineering vessel in a bioreactor.